Soybean seeds loss viability very rapidly during ambient storage in the tropical and sub-tropical environments. In this study, interrelationship between seed coat permeability and viability over periods of ambient storage was assessed using a
set of 217 recombinant inbred lines (RIL) developed from an inter-specific cross between wild type (Glycine soja) accession DC2008-1 and cultivated (G. max) variety DS9712. G. soja seeds were tiny, black, impermeable and highly viable while G. max
seeds were large, yellow, permeable and poorly viable during ambient storage. Seed coat permeability and viability of the fresh, one-year and two-year-stored seeds (stored in room temperature, av. 25±2°C and 65±5% RH) were tested as per standard
protocols in completely randomized design with two replications. Significant variation was found among genotypes for the seed viability, permeability, periods of storage and their interactions. Permeability of the seed coat increased with the period
of storage. In the fresh, one-year and two-yearstored seeds, the seed coat permeability was 62.87, 75.17 and 90.52%, respectively. Viability of the seeds was negatively correlated with period of storage and seed size. In the fresh, one-year and
two-year-stored seeds, average viability was 90.7, 75.6 and 54.1%, respectively. Scanning electron microscopy (SEM) indicated presence of intact hilum, strong hourglass cells and non-cracked seed coat in the highly viable seeds. A set of 24 RILs were
found that maintained higher viability (>80%) with varying degree of permeability after two years of storage. Among the highly viable RILs, more were black seeded. RIL Nos. 7-12-3, 7-24- 1, 13-2-2, 13-31-4 found to maintain both viability and
permeability in higher order during storage and would pave the way for development of soybean genotypes with high viability and permeability.
Yield Stability of Soybean Genotypes in Tropical Environments based on Genotype and Genotype-by-Environment Biplot
