Inheritance of Stripe Rust Resistance among Near‐Isogenic Lines of Spring Wheat

Pathogens are a reason for low yield in common wheat (Triticum aestivum L.). Stripe rust (or yellow rust), caused by the fungus Puccinia striiformis f. sp. tritici (Pst), is one of the most important foliar diseases of wheat. One of the most cost-effective and environmentally sound ways to control stripe rust is to use plant varieties that are resistant to this pathogen. It is an important task for wheat breeders and pathologists to identify new genes and pyramid them in order to achieve high-level, durable resistance to stripe rust. One spring wheat germplasm, HRMSN-81, with resistance to the most dominant races in China, is identified from the CIMMYT breeding lines. To elucidate the genetic basis of its resistance, HRMSN-81 was crossed with susceptible wheat genotype Taichung 29. Seedlings of the parents were tested with Chinese Pst isolates CYR31, CYR32, and CYR33 under controlled greenhouse conditions, and adult plants of the parents and F1, F2, and F2:3 progeny were inoculated with the epidemic stripe rust mixed races, including CYR31, CYR32, and CYR33, in fields under natural infection. Genetic analysis showed that HRMSN-81 has a single dominant gene conferring all-stage resistance, temporarily designated as YrHRMSN-81. Resistance gene analogue polymorphism (RGAP), simple sequence repeat (SSR), target region amplified polymorphism (TRAP), and sequence-related amplified polymorphism (SRAP) techniques in combination with bulked segregant analysis (BSA) were used to identify molecular markers linked to the resistance gene. A linkage map consisting of six RGAP, two SSR, one TRAP, and two SRAP markers was constructed for YrHRMSN-81 using 148 F2 plants. The gene was mapped to chromosome arm 2DS by testing the complete set of nulli-tetrasomic lines and selected ditelosomic lines with two RGAP markers and was further confirmed by two chromosome-specific SSR markers. The results of gene characteristics and chromosome locations indicated that YrHRMSN-81 was probably a new stripe rust resistance gene. The two flanking markers Xwgp-180bp (93% polymorphism rate) and Xwmc453 (91% polymorphism rate) detected 100% polymorphism of the 56 tested wheat genotypes when they were used in combination. The identification of the gene YrHRMSN-81 and the determination of the flanking markers should be useful for rapidly transferring it in wheat breeding programs.

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MOLECULAR GENETIC VARIATION FOR STRIPE RUST RESISTANCE IN SPRING WHEAT

The Pakistan Journal of Agricultural Sciences ◽

10.21162/pakjas/16.2503 ◽

2016 ◽

Vol 53 (01) ◽

pp. 143-150

Author(s):

Muhammad Iqbal ◽

Mahwish Ejaz ◽

Iftikhar Ahmed ◽

Armghan Shahzad ◽

Ghulam M. Ali

Keyword(s):

Genetic Variation ◽

Stripe Rust ◽

Spring Wheat ◽

Rust Resistance ◽

Molecular Genetic ◽

Stripe Rust Resistance ◽

Molecular Genetic Variation

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Chromosomal Location of Genes for Stripe Rust Resistance in Spring Wheat Cultivars Compair, Fielder, Lee, and Lemhi and Interactions of Aneuploid Wheats with Races ofPuccinia striiformis

Phytopathology ◽

10.1094/phyto-85-375 ◽

1995 ◽

Vol 85 (3) ◽

pp. 375 ◽

Cited By ~ 50

Author(s):

X. Chen

Keyword(s):

Stripe Rust ◽

Spring Wheat ◽

Rust Resistance ◽

Chromosomal Location ◽

Stripe Rust Resistance ◽

Wheat Cultivars

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Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s

Theoretical and Applied Genetics ◽

10.1007/s00122-010-1302-0 ◽

2010 ◽

Vol 121 (1) ◽

pp. 195-204 ◽

Cited By ~ 42

Author(s):

P. Cheng ◽

X. M. Chen

Keyword(s):

Stripe Rust ◽

Spring Wheat ◽

Rust Resistance ◽

Molecular Mapping ◽

Wheat Cultivar ◽

Stripe Rust Resistance ◽

Spring Wheat Cultivar

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Identification of Stripe Rust Resistance Loci in U.S. Spring Wheat Cultivars and Breeding Lines Using Genome-Wide Association Mapping and Yr Gene Markers

Plant Disease ◽

10.1094/pdis-11-19-2402-re ◽

2020 ◽

Vol 104 (8) ◽

pp. 2181-2192 ◽

Cited By ~ 3

Author(s):

Lu Liu ◽

Meinan Wang ◽

Zhiwu Zhang ◽

Deven R. See ◽

Xianming Chen

Keyword(s):

Stripe Rust ◽

Spring Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

The United States ◽

Genome Wide Association ◽

Stripe Rust Resistance ◽

Wheat Cultivars ◽

Breeding Lines ◽

Genome Wide

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), poses a major threat to wheat production worldwide, especially in the United States. To identify loci for effective stripe rust resistance in U.S. wheat, a genome-wide association study (GWAS) was conducted using a panel of 616 spring wheat cultivars and breeding lines. The accessions in this panel were phenotyped for stripe rust response in the greenhouse at seedling stage with five predominant and highly virulent races of Pst and in different field environments at adult-plant stage in 2017 and 2018. In total, 2,029 single-nucleotide polymorphism markers that cover the whole genome were generated with genotyping by multiplexed sequencing and used in GWAS. In addition, 23 markers of previously reported resistance genes or quantitative trait loci (QTLs) were used to genotype the population. This spring panel was grouped into three subpopulations based on principal component analysis. A total of 37 genes or QTLs including 10 potentially new QTLs for resistance to stripe rust were detected by GWAS and linked marker tests. The frequencies of the resistance genes or QTLs in various nurseries were determined, indicating different intensities of these genes or QTLs used in breeding programs of different regions. These resistance loci and the information on their markers, effectiveness, and distributions should be useful for improving stripe rust resistance in wheat cultivars.

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Allelic variation at loci controlling stripe rust resistance in spring wheat

Journal of Genetics ◽

10.1007/s12041-014-0413-9 ◽

2014 ◽

Vol 93 (2) ◽

pp. 579-586 ◽

Cited By ~ 1

Author(s):

SANIA BEGUM ◽

MUHAMMAD IQBAL ◽

IFTIKHAR AHMED ◽

MUHAMMAD FAYYAZ ◽

ARMGHAN SHAHZAD ◽

...

Keyword(s):

Stripe Rust ◽

Spring Wheat ◽

Rust Resistance ◽

Allelic Variation ◽

Stripe Rust Resistance

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Genomic Regions Associated With Stripe Rust Resistance Against the Egyptian Race Revealed by Genome-Wide Association Study

10.21203/rs.3.rs-57243/v1 ◽

2020 ◽

Author(s):

Abou-Zeid A. Mohamed ◽

Amira M. I. Mourad

Keyword(s):

Stripe Rust ◽

Spring Wheat ◽

Rust Resistance ◽

Genome Wide Association Study ◽

Snp Markers ◽

Genome Wide Association ◽

Stripe Rust Resistance ◽

Wheat Genotypes ◽

Genome Wide ◽

Resistant Genotypes

Abstract Background: Wheat stripe rust (caused by Puccinia striiformis f. sp. Tritici), is a major disease that causes huge yield damage. New pathogen races appeared in the last few years and caused a broke down in the resistant genotypes. In Egypt, some of the resistant genotypes began to be susceptible to stripe rust in recent years. This situation increases the need to produce new genotypes with durable resistance. Besides, looking for a new resistant source from the available wheat genotypes all over the world help in enhancing the breeding programs. Results: In the recent study, a set of 103-spring wheat genotypes from different fourteen countries were evaluated to their field resistant to stripe rust for two years. These genotypes included 17 Egyptian genotypes from the old and new cultivars. The 103-spring wheat genotypes were reported to be well adapted to the Egyptian environmental conditions. Out of the tested genotypes, eight genotypes from four different countries were found to be resistant in both years. Genotyping was carried out using genotyping-by-sequencing and a set of 26,703 SNPs were used in the genome-wide association study. Five SNP markers, located on chromosomes 2A and 4A, were found to be significantly associated with the resistance in both years. Three gene models associated with disease resistance and underlying these significant SNPs were identified. One immune Iranian genotype, with the highest number of different alleles from the most resistant Egyptian genotypes, was detected. Conclusion: the high variation among the tested genotypes in their resistance to the Egyptian stripe rust race confirming the possible improvement of stripe rust resistance in the Egyptian wheat genotypes. The identified five SNP markers are stable and could be used in marker-assisted selection after validation in different genetic backgrounds. Crossing between the immune Iranian genotype and the Egyptian genotypes will improve stripe rust resistance in Egypt.

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Stripe rust resistance among western Canadian spring wheat and triticale varieties

Canadian Journal of Plant Science ◽

10.4141/cjps2011-252 ◽

2012 ◽

Vol 92 (4) ◽

pp. 713-722 ◽

Cited By ~ 35

Author(s):

H. Randhawa ◽

B. J. Puchalski ◽

M. Frick ◽

A. Goyal ◽

T. Despins ◽

...

Keyword(s):

Stripe Rust ◽

Spring Wheat ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Western Canada ◽

Resistant Varieties ◽

Plant Resistance Gene ◽

Moderately Resistant

Randhawa, H., Puchalski, B. J., Frick, M., Goyal, A., Despins, T., Graf, R. J., Laroche, A. and Gaudet, D. A. 2012. Stripe rust resistance among western Canadian spring wheat and triticale varieties. Can. J. Plant Sci. 92: 713–722. Stripe rust (Puccinia striiformis Westend.) is an important pathogen of wheat in western Canada and worldwide. One hundred and four spring wheat and triticale varieties and cultivars were evaluated for resistance to stripe rust in nurseries at Lethbridge and Creston, BC, during 2009 and 2010. In the Canada Prairie Spring Red (CPSR) wheat class, newer varieties were more resistant compared with many of the older varieties. Among the white Canada Prairie Spring White (CPSW) wheats, Vista was moderately resistant, whereas Snowhite475 and Snowhite476 were susceptible. Little useful resistance was observed within the Canada Western Hard White Spring (CWHWS) class. Sixty percent of the Canada Western Red Spring (CWRS) wheats, including the currently popular varieties Lillian, Harvest and Kane, were resistant. Susceptible CWRS varieties that are extensively seeded in western Canada include AC Barrie, Superb and McKenzie, but also include the recently registered CDC Kernen and Vesper. The varieties were tested for the presence of the stripe rust genes Yr10, Yr17, Yr18 and Yr36 using molecular markers. Much of the stripe rust resistance, particularly in the CWRS, Canada Western Extra Strong (CWES), and CPSR wheat classes was attributed to the presence of adult plant resistance gene Yr18. Yr17 and Yr36 were also detected among CWRS and CWES varieties. However, the absence of markers for known genes in several resistant varieties indicated that uncharacterized genes for stripe rust occur among hexaploid wheats. Durum wheat and triticale varieties were universally resistant with the absence of tested markers. Therefore, there appear to be numerous sources of stripe rust resistance, both characterized and uncharacterized, among western Canadian spring wheat and triticale varieties.

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