Association of the Brazil Nut Protein Gene and Kunitz Trypsin Inhibitor Alleles with Soybean Protease Inhibitor Activity and Agronomic Traits

Five vegetable soybean genotypes were evaluated for agronomic characteristics, functional and antinutritional properties. The experiments were conducted in the field, in the School of Agricultural and Veterinary Sciences, in Jaboticabal, São Paulo State, Brazil. The experimental design was a randomized block design with five treatments (genotypes) and four replications. The genotypes JLM003, JLM004, JLM010, JLM024 and JLM030 were evaluated in the following characteristics: number of pods per plant, number of seeds per pod, fresh weight of pods per plant (g), fresh weight of 100 seeds (g), estimated total yield of immature seeds (kg ha-1), moisture content, proteins, lipids, ash, carbohydrates, isoflavones and Kunitz trypsin inhibitor. In the assessment of the major agronomic traits, in the conditions of this study, the genotype JLM010 was the most recommended for the region of Jaboticabal. This genotype showed better agronomic characteristics of production as a vegetable, producing 136.04 g of fresh weight of pods per plant, 92.52 g of fresh weight/100 seeds and 11.12 t/ha of total immature seeds, and better functional properties.

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Automated Measurement of Protease-Inhibitor Capacity of Serum, by Use of Chymotrypsin and Casein

Clinical Chemistry ◽

10.1093/clinchem/20.3.328 ◽

1974 ◽

Vol 20 (3) ◽

pp. 328-331 ◽

Cited By ~ 4

Author(s):

Julius Wenger ◽

Marian Sundy

Keyword(s):

Protease Inhibitor ◽

Trypsin Inhibitor ◽

Clinical Studies ◽

Automated Measurement ◽

Inhibitor Activity ◽

Chymotrypsin Inhibitor ◽

Casein Hydrolysis ◽

Previous Evidence ◽

Automated Method

Abstract Serum protease inhibitor activity was assessed from the ability of the serum to inhibit casein hydrolysis by α-chymotrypsin (EC 3.4.4.5). Unmodified AutoAnalyzer equipment was used in conjunction with a fluorimetric method for dialyzable tyrosyl peptides. Serum, diluted with tris(hydroxymethyl) aminomethane buffer, was added to a chymotrypsin solution to inhibit its activity. For 72 sera, the automated method was compared with a manual determination of trypsin inhibitor capacity. Results by the two methods correlate well, supporting previous evidence that trypsin and chymotrypsin-inhibitor capacities of serum are related. The present automated method has several advantages that make it useful for clinical studies.

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Effect of Genetic Elimination of Kunitz Trypsin Inhibitor on Agronomic and Quality Traits in Soybean [Glycine max (L.) Merril]]

Legume Research - An International Journal ◽

10.18805/lr-4205 ◽

2020 ◽

Author(s):

Sandeep Kaur Dhaliwal ◽

Satwinder Kaur Dhillon ◽

B. S. Gill ◽

Gurpreet Kaur ◽

Asmita Sirari ◽

...

Keyword(s):

Grain Yield ◽

Trypsin Inhibitor ◽

Nutritional Value ◽

Economic Importance ◽

Trypsin Inhibitor Activity ◽

Kunitz Trypsin Inhibitor ◽

Inhibitor Activity ◽

Nutritional Factors ◽

Days To Maturity ◽

Glycine Max L

Background: Among the anti-nutritional factors present in soybean, kunitz trypsin inhibitor (Kti) serves as major anti-nutrient, retarding the activity of digestive proteases. Genetic removal of Kti allele to develop agronomically desirable genotypes with high nutritional value is major breeding objective in soybean. Methods: The present study was performed on a set of 125 F5 genotypes derived from cross of SL525 (Kti +ve) and NRC101 (null Kti) to investigate the outcomes of genetic removal of Kti allele on characters of economic importance. Results: Comparison of mean of null Kti and Kti +ve plants for various parameters showed that introgression of null Kti allele adversely affected germination and grain yield. Germination, days to maturity, grain yield, plant height and seed weight were positively correlated with trypsin inhibitor activity and days to flowering, oil and protein content and fatty acid were unaffected by the allele present. The utilisation of the identified null kti genotypes would reduce extra cost on heat treatment incurred during soy processing and also boost utilization of soybean for bio-fortification in wheat flour to make chapattis.

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Bowman-Birk inhibitor in soybean: Genetic variability in relation to total trypsin inhibitor activity and elimination of Kunitz trypsin inhibitor

Notulae Scientia Biologicae ◽

10.15835/nsb13110836 ◽

2021 ◽

Vol 13 (1) ◽

pp. 10836

Author(s):

Priyanka MITTAL ◽

Vineet KUMAR ◽

Anita RANI ◽

S. M. GOKHALE

Keyword(s):

Trypsin Inhibitor ◽

Genetic Background ◽

Soy Flour ◽

Trypsin Inhibitor Activity ◽

Recurrent Parent ◽

Kunitz Trypsin Inhibitor ◽

Inhibitor Activity ◽

Commercial Preparation ◽

Defatted Soy Flour ◽

Soybean Genotypes

Bowman-Birk inhibitor (BBI) is a soybean seed serine protease inhibitor whose nutraceutical properties far exceed its anti-nutritional property arising from its trypsin and chymotrypsin inhibitor activity. High BBI soybean genotypes are sought for the commercial preparation of this nutraceutical biomolecule while low BBI content in Kunitz trypsin inhibitor (KTI)-free genetic background is desirable in soymeal manufacturing. In the present investigation, monoclonal antibody assay of 95 soybean genotypes revealed 11-fold genetic variation for BBI concentration. The study led to the identification of 3 very high (>20 mg/g defatted soy flour) and 5 very low BBI (< 4 mg/g defatted soy flour) soybean genotypes. Contribution of BBI to total trypsin inhibition activity ranged from 2.2 to 53.5% with average value of 11.6%. Genotypes with varying level of BBI raised consecutively for two years exhibited non-significant (p>0.05) effect of growing year on the trait. BBI content in BC3F2 derived KTI-free lines was at par with the recurrent parent. Low and high BBI content genotypes with diverse genetic background identified in the study may be exploited to develop mapping population to identify genomic regions underlying BBI in soybean.

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