scholarly journals Predicting Heavy Metal Partition Equilibrium in Soils: Roles of Soil Components and Binding Sites

2018 ◽  
Vol 82 (4) ◽  
pp. 839-849 ◽  
Author(s):  
Shimeng Peng ◽  
Pei Wang ◽  
Lanfang Peng ◽  
Tao Cheng ◽  
Weimin Sun ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Binding Sites ◽  
Partition Equilibrium ◽  
Soil Components ◽  
Metal Partition

scholarly journals Mercury and cadmium trigger expression of the copper importer Ctr1B, which enables Drosophila to thrive on heavy metal-loaded food

2009 ◽  
Vol 390 (2) ◽  
pp. 109-113 ◽  
Author(s):  
Kuppusamy Balamurugan ◽  
Haiqing Hua ◽  
Oleg Georgiev ◽  
Walter Schaffner
Keyword(s):  
Heavy Metal ◽  
Binding Sites ◽  
Sequence Motifs ◽  
Excess Copper ◽  
Number Of Genes ◽  
Zinc Cadmium ◽  
Copper Zinc ◽  
Dna Sequence Motifs ◽  
Transcription Factor 1 ◽  
Transcription Enhancer

Abstract Organisms from insects to mammals respond to heavy metal load (copper, zinc, cadmium, and mercury) by activating the metal-responsive transcription factor 1 (MTF-1). MTF-1 binds to short DNA sequence motifs, termed metal response elements, and boosts transcription of a number of genes, notably those for metallothioneins. In Drosophila, MTF-1 somewhat counter-intuitively also activates transcription of a copper importer gene (Ctr1B) in response to copper starvation. Here, we report that mutant flies lacking Ctr1B are extremely sensitive to cadmium and mercury treatment, but can be rescued by excess copper in the food. We thus propose that copper, by competing for binding sites on cellular proteins, alleviates the toxic effects of mercury and cadmium. Such a scenario also explains a seemingly fortuitous metal response, namely, that cadmium and mercury strongly induce the expression of a Ctr1B reporter gene. Thus, the transcription enhancer/promoter region of the Ctr1B copper importer gene is subject to three modes of regulation. All of them depend on MTF-1 and all make biological sense, namely, (i) induction by copper starvation, (ii) repression by copper abundance, and (iii), as shown here, induction by cadmium or mercury at normal copper supply.

scholarly journals Structural Adaptability of Zinc Binding Sites: Different Structures in Partially, Fully, and Heavy-Metal Loaded States

2009 ◽  
Vol 15 (35) ◽  
pp. 8664-8664 ◽  
Author(s):  
Uwe Heinz ◽  
Lars Hemmingsen ◽  
Martin Kiefer ◽  
Hans-Werner Adolph
Keyword(s):  
Heavy Metal ◽  
Binding Sites ◽  
Zinc Binding ◽  
Structural Adaptability

scholarly journals Characterization of Binding Sites and Optimization of Cell Free Bacteria Condition for Metal Bio-Sorbents

2021 ◽  
Author(s):  
Dr. Mostafa G. Fadl ◽  
Zenat Kamel Mohamed
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Binding Sites ◽  
Metal Uptake ◽  
High Efficiency ◽  
Bacterial Biomass ◽  
Resistant Bacteria ◽  
Bacterial Cells ◽  
Isolation And Identification ◽  
Charge Interaction

Bacteria a Microscopic organisms are the most inexhaustible and flexible of microorganisms and constitute a huge division of the whole living earthly biomass, certain microorganisms were found to amass metallic components at a high limit Was Known as Bacterial Bio-sorption Due to their little size, capacity to become under controlled conditions, and their Accommodation to an extensive variety of ecological situations; Potent metal bio-sorbents among microorganisms, at low pH esteems, cell divider ligands are protonated and contend essentially with metals for official. With expanding pH, more ligands, such as amino and carboxyl groups, could be exposed, leading to attraction between these negative charges and the metals, and consequently increment bio-sorption onto the cell surface. Starting with Isolation and identification of heavy metal-resistant bacteria from rock Ore. Studying Factors Affecting Uranium Bio-sorption, Optimization of bacterial growth conditions and optimum for metal uptake by free and immobilized bacterial cells and Desorption ratio of uranium ions adsorbed by Coli. /alginate, All this evidence suggest that functions groups Represented in our study are responsible for metal uptake in our bacterial biomass beside change in peaks position which assigned for it's groups confirm bio-sorption of metal ions from waste due to ions charge interaction comparing with immobilized we found increase in no of binding sites indicate that immobilized bacterial have high efficiency for metal up take which also change in peaks position which assigned for its groups confirm bio-sorption of metal ions from waste due to ions charge interaction, Where the high bio-sorption yield obtained by bacteria, the Uranium & heavy metal bioremediation process expects microorganisms to be joined to a strong surface.

scholarly journals Using soil heavy metal enrichment and mobility factors to determine potential uptake by vegetables

2011 ◽  
Vol 57 (No. 2) ◽  
pp. 75-80 ◽  
Author(s):  
V.M. Ngole
Keyword(s):  
Heavy Metal ◽  
Metal Uptake ◽  
Metal Enrichment ◽  
Microbial Activities ◽  
Heavy Metal Uptake ◽  
Mobility Factor ◽  
Soil Components ◽  
Cu Uptake ◽  
By Products

This study investigated copper (Cu) and lead (Pb) enrichment factor (EF) and mobility factor (MF) as possible indicators of their uptake by spinach (Spinaceae oleraceae) and carrots (Daucus carota) grown on a sludge-amended luvisol (SAL). Sewage sludge was applied to luvisol at different rates and spinach and carrots planted. Enrichment of Cu and Pb in SAL was determined, and values regressed with those of Cu and Pb concentrations in spinach and carrots. Concentration of Cu and Pb in vegetables was calculated using the regression model obtained, and calculated values compared with actual values. Pb MF were higher than Cu MF but Cu and Pb EFsoil values were < 3.0, indicating minor enrichment from sludge addition. EF had 10% reliability in predicting Cu and Pb uptake in vegetables. MF was more than 70% reliable in predicting carrot Cu uptake and spinach Pb uptake. EF and MF are not effective as predictors of heavy metal uptake by vegetables. The role of other soil components including root exudates and by-products from microbial activities should also be investigated.

The structure and heavy-metal-ion-binding sites of horse spleen apoferritin

1980 ◽  
Vol 8 (5) ◽  
pp. 654-655 ◽  
Author(s):  
GEORGE A. CLEGG ◽  
ROBERT F. D. STANSFIELD ◽  
PHILIP E. BOURNE ◽  
PAULINE M. HARRISON
Keyword(s):  
Heavy Metal ◽  
Binding Sites ◽  
Metal Ion ◽  
Heavy Metal Ion ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites

scholarly journals Genome-wide analysis of metallothionein gene family in maize to reveal its role in development and stress resistance to heavy metal

2022 ◽  
Vol 55 (1) ◽  
Author(s):  
Canhong Gao ◽  
Kun Gao ◽  
Huixian Yang ◽  
Tangdan Ju ◽  
Jingyi Zhu ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Plant Growth ◽  
Stress Resistance ◽  
Binding Sites ◽  
Growth And Development ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Plant Growth And Development ◽  
Specific Expression

Abstract Background Maize (Zea mays L.) is a widely cultivated cereal and has been used as an optimum heavy metal phytoremediation crop. Metallothionein (MT) proteins are small, cysteine-rich, proteins that play important roles in plant growth and development, and the regulation of stress response to heavy metals. However, the MT genes for maize have not been fully analyzed so far. Methods The putative ZmMT genes were identified by HMMER.The heat map of ZmMT genes spatial expression analysis was generated by using R with the log2 (FPKM + 1).The expression profiles of ZmMT genes under three kinds of heavy metal stresses were quantified by using qRT-PCR. The metallothionein proteins was aligned using MAFFT and phylogenetic analysis were constructed by ClustalX 2.1. The protein theoretical molecular weight and pI, subcellular localization, TFs binding sites, were predicted using ProtParam, PSORT, PlantTFDB, respectively. Results A total of 9 ZmMT genes were identified in the whole genome of maize. The results showed that eight of the nine ZmMT proteins contained one highly conserved metallothio_2 domain, while ZmMT4 contained a Metallothio_PEC domain. All the ZmMT proteins could be classified into three major groups and located on five chromosomes. The ZmMT promoters contain a large number of hormone regulatory elements and hormone-related transcription factor binding sites. The ZmMT genes exhibited spatiotemporal specific expression patterns in 23 tissues of maize development stages and showed the different expression patterns in response to Cu, Cd, and Pb heavy metal stresses. Conclusions We identified the 9 ZmMT genes, and explored their conserved motif, tissue expression patterns, evolutionary relationship. The expression profiles of ZmMT genes under three kinds of heavy metal stresses (Cu, Cd, Pb) were analyzed. In summary, the expression of ZmMTs have poteintial to be regulated by hormones. The specific expression of ZmMTs in different tissues of maize and the response to different heavy metal stresses are revealed that the role of MT in plant growth and development, and stress resistance to heavy metals.

scholarly journals Heavy metal adsorption using structurally preorganized adsorbent

RSC Advances ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 7259-7264 ◽  
Author(s):  
Shuai Liang ◽  
Shengguang Cao ◽  
Changrong Liu ◽  
Shah Zeb ◽  
Yu Cui ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Functional Groups ◽  
Binding Sites ◽  
Alkali Metals ◽  
Amide Group ◽  
Metal Adsorption ◽  
Heavy Metal Adsorption ◽  
Coordination Unit ◽  
Alkali Soil ◽  
Soil Metals

The adsorbent had two types of functional groups and six binding sites in one coordination unit. The presence of the amide group shows good anti-interference to alkali metals and alkali soil metals.

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