Sustained Secretion of the Antimicrobial Peptide S100A7 Is Mediated by the Wound Healing Machinery

2019 ◽  
Author(s):  
Tanay Bhatt ◽  
Mruthyunjaya MS ◽  
Aishwarya Bhosale ◽  
Bhavya Bajantri ◽  
Abrar Rizvi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antimicrobial Peptide

Antimicrobial Peptide-Loaded Pectolite Nanorods for Enhancing Wound-Healing and Biocidal Activity of Titanium

2021 ◽  
Author(s):  
Lan Zhang ◽  
Yang Xue ◽  
Sanjana Gopalakrishnan ◽  
Kai Li ◽  
Yong Han ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antimicrobial Peptide ◽  
Biocidal Activity

scholarly journals The Designer Antimicrobial Peptide A-hBD-2 Facilitates Skin Wound Healing by Stimulating Keratinocyte Migration and Proliferation

2018 ◽  
Vol 51 (2) ◽  
pp. 647-663 ◽  
Author(s):  
Bobin Mi ◽  
Jing Liu ◽  
Yi Liu ◽  
Liangcong Hu ◽  
Yukun Liu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Antimicrobial Peptide ◽  
Structural Stability ◽  
Cell Counting ◽  
Sprague Dawley ◽  
Skin Wound Healing ◽  
High Sodium

Background/Aims: Antimicrobial peptides are effective promoters of wound healing but are susceptible to degradation. In this study, we replaced the GIGDP unit on the N-terminal of the endogenous human antimicrobial peptide hBD-2 with APKAM to produce A-hBD-2 and analyzed the effect on wound healing both in vitro and in vivo. Methods: The effects of A-hBD-2 and hBD-2 on cytotoxicity and proliferation in keratinocytes were assessed by Cell Counting Kit-8 assay. The structural stability and antimicrobial activity of hBD-2 and A-hBD-2 were evaluated against Staphylococcus aureus. RNA and proteins levels were evaluated by real-time PCR and western blotting, respectively. Cell migration was evaluated using a transwell assay. Cell cycle analysis was performed by flow cytometry. Wound healing was assessed in Sprague-Dawley rats. Epidermal thickness was evaluated by hematoxylin and eosin staining. Results: We found that hBD-2 exhibited cytotoxicity at high concentrations and decreased the structural stability in the presence of high sodium chloride concentrations. A-hBD-2 exhibited increased structural stability and antimicrobial activity, and had lower cytotoxicity in keratinocytes. A-hBD-2 increased the migration and proliferation of keratinocytes via phosphorylation of EGFR and STAT3 and suppressed terminal differentiation of keratinocytes. We also found that A-hBD-2 elicited mobilization of intracellular Ca2+ and stimulated keratinocytes to produce pro- and anti-inflammatory cytokines and chemokines via phospholipase C activation. Furthermore, A-hBD-2 promoted wound healing in vivo. Conclusion: Our data suggest that A-hBD-2 may be a promising candidate therapy for wound healing.

scholarly journals The Antimicrobial Peptide Human β-Defensin-3 Accelerates Wound Healing by Promoting Angiogenesis, Cell Migration, and Proliferation Through the FGFR/JAK2/STAT3 Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Miho Takahashi ◽  
Yoshie Umehara ◽  
Hainan Yue ◽  
Juan Valentin Trujillo-Paez ◽  
Ge Peng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Growth Factors ◽  
Growth Factor ◽  
Antimicrobial Peptide ◽  
Fibroblast Growth Factor ◽  
Human Fibroblasts ◽  
Janus Kinase ◽  
Fibroblast Growth ◽  
Angiogenic Growth Factors

In addition to its antimicrobial activity, the skin-derived antimicrobial peptide human β-defensin-3 (hBD-3) promotes keratinocyte proliferation and migration to initiate the wound healing process; however, its effects on fibroblasts, which are the major cell type responsible for wound healing, remain unclear. We investigated the role of hBD-3 in cell migration, proliferation and production of angiogenic growth factors in human fibroblasts and evaluated the in vivo effect of hBD-3 on promoting wound healing and angiogenesis. Following hBD-3 treatment, the mouse wounds healed faster and showed accumulation of neutrophils and macrophages in the early phase of wound healing and reduction of these phagocytes 4 days later. hBD-3-treated wounds also displayed an increased number of fibroblasts and newly formed vessels compared to those of the control mice. Furthermore, the expression of various angiogenic growth factors was increased in the hBD-3-treated wounds. Additionally, in vitro studies demonstrated that hBD-3 enhanced the secretion of angiogenic growth factors such as fibroblast growth factor, platelet-derived growth factor and vascular endothelial growth factor and induced the migration and proliferation of human fibroblasts. The hBD-3-mediated activation of fibroblasts involves the fibroblast growth factor receptor 1 (FGFR1)/Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathways, as evidenced by the inhibitory effects of pathway-specific inhibitors. We indeed confirmed that hBD-3 enhanced the phosphorylation of FGFR1, JAK2 and STAT3. Collectively, the current study provides novel evidence that hBD-3 might be a potential candidate for the treatment of wounds through its ability to promote wound healing, angiogenesis and fibroblast activation.

Antimicrobial Peptide‐Based Electrospun Fibers for Wound Healing Applications

2019 ◽  
Vol 19 (9) ◽  
pp. 1800488 ◽  
Author(s):  
Alexander Dart ◽  
Mrinal Bhave ◽  
Peter Kingshott
Keyword(s):  
Wound Healing ◽  
Antimicrobial Peptide ◽  
Electrospun Fibers

Antimicrobial peptide modification enhances the gene delivery and bactericidal efficiency of gold nanoparticles for accelerating diabetic wound healing

2018 ◽  
Vol 6 (10) ◽  
pp. 2757-2772 ◽  
Author(s):  
Song Wang ◽  
Chang Yan ◽  
Ximu Zhang ◽  
Dezhi Shi ◽  
Luxiang Chi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Wound Healing ◽  
Gene Delivery ◽  
Bacterial Infection ◽  
Antimicrobial Peptide ◽  
Diabetic Wound ◽  
Peptide Modification ◽  
Diabetic Wound Healing ◽  
Bactericidal Efficiency ◽  
Impaired Angiogenesis

Impaired angiogenesis and bacterial infection have increasingly been implicated as the major causes of delayed diabetic wound healing.

scholarly journals Erratum to: Efficacy of the designer antimicrobial peptide SHAP1 in wound healing and wound infection

Amino Acids ◽  
2014 ◽  
Vol 46 (10) ◽  
pp. 2345-2345 ◽  
Author(s):  
Da Jung Kim ◽  
Young Woong Lee ◽  
Myung Keun Park ◽  
Ju Ri Shin ◽  
Ki Jung Lim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antimicrobial Peptide ◽  
Wound Infection

scholarly journals The Frog Skin-Derived Antimicrobial Peptide Esculentin-1a(1-21)NH2 Promotes the Migration of Human HaCaT Keratinocytes in an EGF Receptor-Dependent Manner: A Novel Promoter of Human Skin Wound Healing?

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128663 ◽  
Author(s):  
Antonio Di Grazia ◽  
Floriana Cappiello ◽  
Akiko Imanishi ◽  
Arianna Mastrofrancesco ◽  
Mauro Picardo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antimicrobial Peptide ◽  
Human Skin ◽  
Frog Skin ◽  
Egf Receptor ◽  
Skin Wound ◽  
Dependent Manner ◽  
Hacat Keratinocytes ◽  
Skin Wound Healing
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