Biofunctions of antimicrobial peptide-conjugated alginate/hyaluronic acid/collagen wound dressings promote wound healing of a mixed-bacteria-infected wound

Bacterial infection poses a significant threat to wound healing, and the preparation of novel wound dressings is a concern. However, currently reported dressings serve as traditional physical barriers or functioned...

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The dual delivery of growth factors and antimicrobial peptide by PLGA/GO composite biofilms to promote skin-wound healing

New Journal of Chemistry ◽

10.1039/c9nj05389a ◽

2020 ◽

Vol 44 (4) ◽

pp. 1463-1476

Author(s):

Ziyan Zhang ◽

Shicheng Zhou ◽

Yanzhe Zhang ◽

Dankai Wu ◽

Xiaoyu Yang

Keyword(s):

Wound Healing ◽

Growth Factors ◽

Antimicrobial Peptide ◽

Medical Devices ◽

Skin Wound ◽

Skin Wound Healing ◽

Promote Wound Healing ◽

Biodegradable Biomaterials

Biodegradable biomaterials coated with active factors are effective medical devices to promote wound healing.

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Wound Healing and Anti-inflammatory Effects of Topical Hyaluronic Acid Injection in Surgical-Site Infection Caused by Staphylococcus aureus

The International Journal of Lower Extremity Wounds ◽

10.1177/1534734617714142 ◽

2017 ◽

Vol 16 (3) ◽

pp. 202-207 ◽

Cited By ~ 8

Author(s):

Jin Hyung Park ◽

Eon Ju Park ◽

Hyung Suk Yi

Keyword(s):

Staphylococcus Aureus ◽

Wound Healing ◽

Hyaluronic Acid ◽

Surgical Site Infection ◽

Bacterial Count ◽

Control Group ◽

Site Infection ◽

Promote Wound Healing ◽

Gross Pathology ◽

Anti Inflammatory

Surgical-site infection (SSI) is a common postoperative complication, primarily caused by Staphylococcus aureus. S aureus produces hyaluronidase which degrades hyaluronic acid (HA). HA prevents bacterial proliferation and has anti-inflammatory effects to promote wound healing. We evaluated the effect of HA injection with systemic antibiotics for prevention and treatment of SSIs caused by S aureus. An open wound was created on the dorsum of 40 rats. The wound bed was sutured with S aureus inoculated thread. The test group was injected with HA (HA group), and the control group received a subcutaneous injection of normal saline (NS group). All groups were then treated with intraperitoneal cefazolin injection. The sutures were removed 2 days after the procedure. Gross pathology, bacterial count, and wound histology were assessed at days 2, 4, 6, and 8 postprocedure. The HA group showed a significant reduction in the wound area compared with the control group on gross pathology (at days 8 postprocedure, 36.54% ± 6.12% vs 50.59% ± 5.50%, P < .001). The HA group showed significantly better wound healing than the control group on histological analysis, including assessment of abscess, neutrophilic infiltration, and necrosis (4.2 ± 1.2 vs 11.5 ± 2.1, P < .001). The HA group showed a lower bacterial count compared with the NS group, but the result was not significant statistically (at days 6 postprocedure, 5.11 ± 0.31 vs 5.91 ± 0.35 logCFU/mL, P = .706). In conclusion, immediate local injection of HA in wounds can reduce SSI occurrence and promote wound healing in an animal model.

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In Vivo Assessments of Minocycline combined Hyaluronic Acid-mediated Ultrasound Therapy of Infected Wound in Rats

10.1101/450585 ◽

2018 ◽

Author(s):

Hongbo Zhang ◽

Deshu Zhuang

Keyword(s):

Staphylococcus Aureus ◽

Wound Healing ◽

Hyaluronic Acid ◽

Visual Inspection ◽

Control Group ◽

Ultrasound Therapy ◽

Infected Wound ◽

Hematoxylin And Eosin ◽

Skin Scar

AbstractObjectivesThe purpose of our research was to examine the effects of Minocycline combined hyaluronic acid (HA)-mediated Ultrasound therapy of infected wound in wister rats.Methods40 female wister rats were made wound on the two side of the backbone, then infected in Staphylococcus aureus at the comic for three times. then, they are divided into four groups: control group, minocycline combined HA alone, ultasound alone, minocycline combined HA-mediated ultasound group, respective. After 3 times of treatments, the rats were killed and made into specimens. Assessments consisted of visual inspection in the change of the skin, scar formation pathological morphology by hematoxylin and eosin(HE) stain with optical microscopy, IL-1B assaying and TNF-a were performed.ResultCompared with control group, minocycline combined HA alone, ultasound alone, minocycline combined HA-mediated ultasound group all have effect for wound healing, there was a obvious improvement in all parameters over the duration of the experiment(P<0.05). Compared with the control group, minocycline combined HA-mediated ultasound group indicated less inflammation cells (P<0.001) and the reduce of and IL-1B and TNF-a (P<0.001).ConclusionMinocycline combined HA-mediated ultrasound can accelerate tissue regrowth, which exert significant benefits in healing the wounds.

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THE POSSIBILITY OF USING THE POLYMER FOR THE TREATMENT OF FULL NANOBIOCOMPOSITES SKIN DEFECTS

Vestnik of Russian military medical Academy ◽

10.17816/brmma12164 ◽

2017 ◽

Vol 19 (1) ◽

pp. 46-50

Author(s):

Evgeniy V Zinov'ev ◽

Marat S Asadulaev ◽

Denis V Kostyakov ◽

Kamil F Osmanov ◽

Roman G Stoyanovskiy ◽

...

Keyword(s):

Wound Healing ◽

Hyaluronic Acid ◽

Reparative Regeneration ◽

Wound Dressings ◽

Skin Defect ◽

Skin Wounds ◽

Mechanical Reproduction ◽

Significant Acceleration ◽

Hyaluronic Acid Hydrogel ◽

Regeneration Processes

The results of the experimental evaluation of the effectiveness of wound dressings based on chitosan nanofibres and copolyamide with treatment of full mechanical skin wounds. To develop their own original technique of mechanical reproduction all layers of the skin wounds in the experiment. It was found that application of wound dressings based on aliphatic copolyamide and chitosan can significantly optimize reparative regeneration processes in the area all layers of the skin defect, thus reducing the time stated scab rejection and wound healing, respectively, 7.5 and 11.2 days (p 0,05). It is shown that the use of coatings based on hyaluronic acid hydrogel is also accompanied by a significant acceleration in the zone histogenesis all layers of the blemishes, shortening the healing period of the final 21% (p 0,05) (4 figs, 1 table, bibliography: 14 refs).

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Hyaluronic Acid Reduces Bacterial Fouling and Promotes Fibroblasts’ Adhesion onto Chitosan 2D-Wound Dressings

International Journal of Molecular Sciences ◽

10.3390/ijms21062070 ◽

2020 ◽

Vol 21 (6) ◽

pp. 2070 ◽

Cited By ~ 2

Author(s):

Ilaria Silvestro ◽

Mariangela Lopreiato ◽

Anna Scotto d’Abusco ◽

Valerio Di Lisio ◽

Andrea Martinelli ◽

...

Keyword(s):

Wound Healing ◽

Hyaluronic Acid ◽

Biofilm Formation ◽

Transmission Rate ◽

Wound Care ◽

Chemical Properties ◽

Infectious Complications ◽

Wound Dressings ◽

Physico Chemical ◽

Wound Healing Activity

Wound healing is a dynamic process that can be seriously delayed by many factors including infectious complications. The development of dressings with intrinsic wound healing activity and/or releasing bioactive compounds may help with addressing such an issue. In this study, hyaluronic acid (HA) at different percentages (1–35%) was used to modify chitosan (CS) biological and physico-chemical properties in order to obtain 2D-matrices able to promote healing and protect from infection. HA incorporation in the CS matrix decreased film transparency and homogeneity, but improved film water uptake and surface wettability. The water vapor transmission rate (WVTR) increased up to a 5% HA content, where it reached the highest value (672 g/m2 day), and decreased for higher HA contents. At all of the tested HA concentrations, HA affected mechanical properties providing matrices more flexible than pure CS with benefit for wound care. Pure CS films permitted S. epidermidis adhesion and biofilm formation. That was not true for CS/HA matrices, where HA at concentrations equal to or greater than 5% was able to avoid S. epidermidis adhesion. Fibroblasts adhesion also took benefit from the HA presence in the film, especially at 5% content, where the best adhesion and proliferation was found.

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