Bone Mesenchymal Stem Cell Derived-Exosomal microRNA-29b-3p Ameliorates Hypoxic-Ischemic Brain Injury by Inhibiting Apoptosis and Promoting Angiogenesis Through PTEN and Akt Signaling Pathway

2019 ◽  
Author(s):  
Kun Hou ◽  
Guichen Li ◽  
Jinchuan Zhao ◽  
Baofeng Xu ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Injury ◽  
Mesenchymal Stem Cell ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Ischemic Brain Injury ◽  
Akt Signaling Pathway ◽  
Ischemic Brain ◽  
Hypoxic Ischemic Brain Injury ◽  
Exosomal Microrna

scholarly journals RETRACTED ARTICLE:Bone mesenchymal stem cell-derived exosomal microRNA-29b-3p prevents hypoxic-ischemic injury in rat brain by activating the PTEN-mediated Akt signaling pathway

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Kun Hou ◽  
Guichen Li ◽  
Jinchuan Zhao ◽  
Baofeng Xu ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Brain Injury ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Luciferase Reporter ◽  
Ischemic Brain Injury ◽  
Akt Signaling Pathway ◽  
Ischemic Brain ◽  
Hypoxic Ischemic Brain Injury ◽  
The Brain

Abstract Background Mesenchymal stem cells (MSCs) are suspected to exert neuroprotective effects in brain injury, in part through the secretion of extracellular vesicles like exosomes containing bioactive compounds. We now investigate the mechanism by which bone marrow MSCs (BMSCs)-derived exosomes harboring the small non-coding RNA miR-29b-3p protect against hypoxic-ischemic brain injury in rats. Methods We established a rat model of middle cerebral artery occlusion (MCAO) and primary cortical neuron or brain microvascular endothelial cell (BMEC) models of oxygen and glucose deprivation (OGD). Exosomes were isolated from the culture medium of BMSCs. We treated the MCAO rats with BMSC-derived exosomes in vivo, and likewise the OGD-treated neurons and BMECs in vitro. We then measured apoptosis- and angiogenesis-related features using TUNEL and CD31 immunohistochemical staining and in vitro Matrigel angiogenesis assays. Results The dual luciferase reporter gene assay showed that miR-29b-3p targeted the protein phosphatase and tensin homolog (PTEN). miR-29b-3p was downregulated and PTEN was upregulated in the brain of MCAO rats and in OGD-treated cultured neurons. MCAO rats and OGD-treated neurons showed promoted apoptosis and decreased angiogenesis, but overexpression of miR-29b-3p or silencing of PTEN could reverse these alterations. Furthermore, miR-29b-3p could negatively regulate PTEN and activate the Akt signaling pathway. BMSCs-derived exosomes also exerted protective effects against apoptosis of OGD neurons and cell apoptosis in the brain samples from MCAO rats, where we also observed promotion of angiogenesis. Conclusion BMSC-derived exosomal miR-29b-3p ameliorates ischemic brain injury by promoting angiogenesis and suppressing neuronal apoptosis, a finding which may be of great significance in the treatment of hypoxic-ischemic brain injury.

scholarly journals Mesenchymal Stem-Cell Transplantation for Hypoxic-Ischemic Brain Injury in Neonatal Rat Model

2010 ◽  
Vol 67 (1) ◽  
pp. 42-46 ◽  
Author(s):  
Jin A Lee ◽  
Beyong Il Kim ◽  
Chris Hyunchul Jo ◽  
Chang Won Choi ◽  
Ee-Kyung Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Injury ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Transplantation ◽  
Neonatal Rat ◽  
Ischemic Brain Injury ◽  
Mesenchymal Stem Cell Transplantation ◽  
Ischemic Brain ◽  
Hypoxic Ischemic Brain Injury ◽  
Neonatal Rat Model

scholarly journals Interaction between hypothermia and delayed mesenchymal stem cell therapy in neonatal hypoxic-ischemic brain injury

2018 ◽  
Vol 70 ◽  
pp. 118-130 ◽  
Author(s):  
Josephine Herz ◽  
Christian Köster ◽  
Barbara S. Reinboth ◽  
Mark Dzietko ◽  
Wiebke Hansen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Injury ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Ischemic Brain Injury ◽  
Ischemic Brain ◽  
Mesenchymal Stem Cell Therapy ◽  
Hypoxic Ischemic Brain Injury

Ischemic brain injury: A consortium analysis of key factors involved in mesenchymal stem cell-mediated inflammatory reduction

2013 ◽  
Vol 534 (1-2) ◽  
pp. 88-97 ◽  
Author(s):  
Colin P. McGuckin ◽  
Marcin Jurga ◽  
Anne-Marie Miller ◽  
Anna Sarnowska ◽  
Marc Wiedner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Injury ◽  
Mesenchymal Stem Cell ◽  
Ischemic Brain Injury ◽  
Key Factors ◽  
Ischemic Brain

miR-150-3p Enhances Neuroprotective Effects of Neural Stem Cell Exosomes after Hypoxic-Ischemic Brain Injury by Targeting CASP2

2021 ◽  
Author(s):  
hongcheng Luo ◽  
Guangbin Ye ◽  
Yu Liu ◽  
Deyou Huang ◽  
Qisheng Luo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Injury ◽  
Neural Stem Cell ◽  
Ischemic Brain Injury ◽  
Neuroprotective Effects ◽  
Ischemic Brain ◽  
Hypoxic Ischemic Brain Injury
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