Injectable Nanostructured Colloidal Gels Resembling Native Nucleus Pulposus as Carriers of Mesenchymal Stem Cell for the Repair of Degenerated Intervertebral Discs

2021 ◽  
Author(s):  
Yu Wang ◽  
Yang Zhang ◽  
Kaiwen Chen ◽  
Fei Shao ◽  
Ye Wu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Nucleus Pulposus ◽  
Intervertebral Discs ◽  
Colloidal Gels ◽  
Degenerated Intervertebral Discs

scholarly journals Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Kaishun Xia ◽  
Jian Zhu ◽  
Jianming Hua ◽  
Zhe Gong ◽  
Chao Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Water Content ◽  
Nucleus Pulposus ◽  
Intervertebral Discs ◽  
Disc Height ◽  
Induced Pluripotent ◽  
Histology And Immunohistochemistry ◽  
Degenerated Intervertebral Discs

Background. Cell replacement therapy is an attractive alternative for treating degenerated intervertebral discs (IVDs), which are related to the reduction of nucleus pulposus-like cells (NP-lCs) and the loss of the extracellular matrix. Induced pluripotent stem cells (iPSCs) which resemble embryonic stem cells are considered to be a potential resource for restoring NP-lCs and disc homeostasis. Here, we proposed an efficient two-step differentiation protocol of human iPSCs into NP-lCs and continuously tested their in vivo ability to regenerate IVDs. Methods. A polymeric gelatin microsphere (GM) was generated for sustained release of growth and differentiation factor-5 (GDF-5) and as a cell delivery vehicle of NP-lCs. By injecting NP-lC-seeded GDF-5-loaded GMs into the rat coccygeal intervertebral discs, the disc height and water content were examined with the molybdenum target radiographic imaging test and magnetic resonance imaging examination. Histology and immunohistochemistry results were shown with H&E, S-O-Fast Green, and immunohistochemistry staining. Results. We demonstrated that the injection of NP-lC-seeded GDF-5-loaded GMs could reverse IDD in a rat model. The imaging examination indicated that disc height recovered and water content increased. Histology and immunohistochemistry results indicated that the NP cells as well as their extracellular matrix were partially restored. Conclusions. The results suggest that NP-lC-seeded GDF-5-loaded GMs could partially regenerate degenerated intervertebral discs after transplantation into rat coccygeal intervertebral discs. Our study will help develop a promising method of stem cell-based therapy for IDD.

Reconstitution of Nucleus Pulposus Cell Matrix Niche in 3D Configuration for Mesenchymal Stem Cell Differentiation

2012 ◽  
Vol 2 (1_suppl) ◽  
pp. s-0032-1319994-s-0032-1319994
Author(s):  
M. Yuan ◽  
C. W. Yeung ◽  
B. P. Chan
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cell ◽  
Stem Cell Differentiation ◽  
Mesenchymal Stem Cell Differentiation ◽  
Cell Matrix

P86. Mesenchymal Stem Cell and Nucleus Pulposus Cell Interactions: Differentiation, Stimulatory Effects, and Cell Fusion

2006 ◽  
Vol 6 (5) ◽  
pp. 124S-125S
Author(s):  
Gianluca Vadalà ◽  
James Kang ◽  
Lars Gilbertson
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Nucleus Pulposus ◽  
Cell Fusion ◽  
Nucleus Pulposus Cell ◽  
Cell Interactions

scholarly journals Mesenchymal Stem Cell-Specific and Preosteoblast-Specific Ablation of TSC1 in Mice Lead to Severe and Slight Spinal Dysplasia, Respectively

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Cheng Yang ◽  
Jianwen Liao ◽  
Pinglin Lai ◽  
Hai Huang ◽  
Shicai Fan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Body Size ◽  
Mesenchymal Stem Cell ◽  
Endochondral Ossification ◽  
Intervertebral Discs ◽  
Tsc1 Gene ◽  
Spine Development ◽  
Old Mice ◽  
Deficient Mice ◽  
Specific Deletion

Background. TSC1-related signaling plays a pivotal role in intramembranous and endochondral ossification processes during skeletogenesis. This study was aimed at determining the significance of the TSC1 gene at different stages of spinal development. Materials and Methods. TSC1-floxed mice (TSC1flox/flox) were crossed with Prrx1-Cre or BGLAP-Cre transgenic mice or mesenchymal stem cell- and osteoblast-specific TSC1-deficient mice, respectively. Somatic and vertebral differences between WT and Prrx1-TSC1 null mice were examined at 4 weeks after birth. Results. No apparent body size abnormalities were apparent in newborn and 4-week- to 2-month-old mice with BGLAP-Cre driver-depleted TSC1. Vertebral and intervertebral discs displayed strong dysplasia in Prrx1-TSC1 null mice. In contrast, vertebrae were only slightly affected, and intervertebral discs from skeletal preparations displayed no apparent changes in BGLAP-TSC1 null mice. Conclusion. Our data suggest that the TSC1 gene is crucial for endochondral ossification during postnatal spine development but plays discriminative roles at different stages. Mesenchymal stem cell-specific ablation of TSC1 led to severe spinal dysplasia at early stages of endochondral ossification while osteoblast-specific deletion of TSC1 affected vertebrae slightly and had no detectable effects on intervertebral discs.

Stem cell regeneration of degenerated intervertebral discs: Current status

2008 ◽  
Vol 12 (2) ◽  
pp. 83-88 ◽  
Author(s):  
Stephen M. Richardson ◽  
Judith A. Hoyland
Keyword(s):  
Stem Cell ◽  
Intervertebral Discs ◽  
Current Status ◽  
Cell Regeneration ◽  
Degenerated Intervertebral Discs

The effect of intervertebral disc degenerative change on biological characteristics of nucleus pulposus mesenchymal stem cell: an in vitro study in rats

2019 ◽  
Vol 60 (4) ◽  
pp. 376-388 ◽  
Author(s):  
Yang Liu ◽  
Yan Li ◽  
Ze-Nan Huang ◽  
Ze-Yu Wang ◽  
Li-Ping Nan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Degenerative Change ◽  
In Vitro Study ◽  
Biological Characteristics ◽  
Vitro Study

scholarly journals Identification of critical genes in nucleus pulposus cells isolated from degenerated intervertebral discs using bioinformatics analysis

2017 ◽  
Vol 16 (1) ◽  
pp. 553-564 ◽  
Author(s):  
Zhuangchen Zhu ◽  
Guang Chen ◽  
Wei Jiao ◽  
Defeng Wang ◽  
Yan Cao ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Bioinformatics Analysis ◽  
Intervertebral Discs ◽  
Nucleus Pulposus Cells ◽  
Degenerated Intervertebral Discs
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