scholarly journals 2P240 Single-molecule tracking and detection of transient colocalization of cholesterol in the live-cell plasma membrane(Cell biological problems-adhesion, motility, cytoskeleton, signaling, and membrane,Oral Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S173
Author(s):  
Yuri L. Nemoto ◽  
Ikuko Koyama-Honda ◽  
Fumiyuki Sanematsu ◽  
Yukiko Shimada ◽  
Masako Yahara ◽  
...  
2015 ◽  
Vol 108 (2) ◽  
pp. 322a
Author(s):  
Andreas Anderluh ◽  
Enrico Klotzsch ◽  
Vivek Kumar ◽  
Amy H. Newman ◽  
Harald H. Sitte ◽  
...  

2014 ◽  
Vol 289 (7) ◽  
pp. 4387-4394 ◽  
Author(s):  
Andreas Anderluh ◽  
Enrico Klotzsch ◽  
Alexander W. A. F. Reismann ◽  
Mario Brameshuber ◽  
Oliver Kudlacek ◽  
...  

2019 ◽  
Vol 116 (50) ◽  
pp. 25269-25277 ◽  
Author(s):  
Nairi Pezeshkian ◽  
Nicholas S. Groves ◽  
Schuyler B. van Engelenburg

The HIV-1 envelope glycoprotein (Env) is sparsely incorporated onto assembling virus particles on the host cell plasma membrane in order for the virus to balance infectivity and evade the immune response. Env becomes trapped in a nascent particle on encounter with the polymeric viral protein Gag, which forms a dense protein lattice on the inner leaflet of the plasma membrane. While Env incorporation efficiency is readily measured biochemically from released particles, very little is known about the spatiotemporal dynamics of Env trapping events. Herein, we demonstrate, via high-resolution single-molecule tracking, that retention of Env trimers within single virus assembly sites requires the Env cytoplasmic tail (CT) and the L12 residue in the matrix (MA) domain of Gag but does not require curvature of the viral lattice. We further demonstrate that Env trimers are confined to subviral regions of a budding Gag lattice, supporting a model where direct interactions and/or steric corralling between the Env-CT and a lattice of MA trimers promote Env trapping and infectious HIV-1 assembly.


2010 ◽  
Vol 98 (3) ◽  
pp. 426a
Author(s):  
Mario Brameshuber ◽  
Julian Weghuber ◽  
Verena Ruprecht ◽  
Imre Gombos ◽  
Ibolya Horváth ◽  
...  

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Eva Sevcsik ◽  
Mario Brameshuber ◽  
Martin Fölser ◽  
Julian Weghuber ◽  
Alf Honigmann ◽  
...  

2019 ◽  
Vol 294 (14) ◽  
pp. 5632-5642 ◽  
Author(s):  
Anand Kant Das ◽  
Oliver Kudlacek ◽  
Florian Baumgart ◽  
Kathrin Jaentsch ◽  
Thomas Stockner ◽  
...  

Nanoscale ◽  
2020 ◽  
Vol 12 (35) ◽  
pp. 18476-18477
Author(s):  
Andres I. König ◽  
Raya Sorkin ◽  
Ariel Alon ◽  
Dikla Nachmias ◽  
Kalyan Dhara ◽  
...  

Correction for ‘Live cell single molecule tracking and localization microscopy of bioorthogonally labeled plasma membrane proteins’ by Andres I. König et al., Nanoscale, 2020, 12, 3236–3248, DOI: 10.1039/C9NR08594G.


2010 ◽  
Vol 285 (53) ◽  
pp. 41765-41771 ◽  
Author(s):  
Mario Brameshuber ◽  
Julian Weghuber ◽  
Verena Ruprecht ◽  
Imre Gombos ◽  
Ibolya Horváth ◽  
...  

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