scholarly journals Combination of Zeolite, Charcoal and Water Spinach as Integrated Filters to Reduce Ammonia Level in Aquaponic System

SCISCITATIO ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 7-15
Author(s):  
Fista Yohana Tanaya ◽  
Kisworo ◽  
Guruh Prihatmo
Keyword(s):  
Ammonia Concentration ◽  
Ammonia Level ◽  
Water Sampling ◽  
Water Spinach ◽  
Integrated Filters ◽  
Recirculation System ◽  
Research Period

Aquaponic is a combination of aquaculture and hydroponic plants in the recirculation system. The aquaponic system has a constraint in the form of ammonia which is produced by fish metabolism. In order to increase the productivity of fish and plants in aquaponics, an approach by integrating filters and biofilter could be used to reduce ammonia waste. The aim of this research was to study the use of zeolite, charcoal, and water spinach as components of integrated filters to reduce ammonia concentration in an aquaponic system. This research was conducted for four weeks with three repetitions of water sampling sourced from pond, filters, and output. The results of this study indicated that the use of combination of zeolite, charcoal and water spinach as componentof integrated filters can reduce ammonia throughout the research period.  

scholarly journals Effects of dietary energy and nitrogen supplements on rumen fermentation and protozoa population in buffalo and zebu cattle

2010 ◽  
Vol 39 (3) ◽  
pp. 549-555 ◽  
Author(s):  
Raul Franzolin ◽  
Fabrício Pini Rosales ◽  
Weber Vilas Bôas Soares
Keyword(s):  
Soybean Meal ◽  
Volatile Fatty Acids ◽  
Animal Species ◽  
Latin Square ◽  
Nitrogen Sources ◽  
Ammonia Concentration ◽  
Ammonia Level ◽  
Zebu Cattle ◽  
Rumen Protozoa ◽  
Citrus Pulp

The effects were assessed of two energy sources in concentrate (ground grain corn vs. citrus pulp) and two nitrogen sources (soybean meal vs. urea) on rumen metabolism in four buffaloes and four zebu cattle (Nellore) with rumen cannula and fed in a 4 × 4 Latin square design with feeds containing 60% sugar cane. Energy supplements had no effect on the rumen ammonia concentration in cattle, but ground grain corn promoted higher ammonia level than citrus pulp in buffalo. Urea produced higher ammonia level than soybean meal in both animal species. On average, the buffaloes maintained a lower rumen ammonia concentration (11.7 mg/dL) than the cattle (14.5 mg/dL). Buffaloes had lower production of acetic acid than cattle (58.7 vs. 61.6 mol/100 mol) and higher of propionic acid (27.4 vs. 23.6 mol/100 mol). There was no difference in the butyric acid production between the buffaloes (13.6 mol/100 mol) and cattle (14.8 mol/100 mol) and neither in the total volatile fatty acids concentration (82.5 vs. 83.6 mM, respectively). The energy or nitrogen sources had no effect on rumen protozoa count in either animal species. The zebu cattle had higher rumen protozoa population (8.8 × 10(5)/mL) than the buffaloes (6.1 × 10(5)/mL). The rumen protozoa population differed between the animal species, except for Dasytricha and Charonina. The buffaloes had a lower Entodinium population than the cattle (61.0 vs 84.9%, respectively) and a greater percentage of species belonging to the Diplodiniinae subfamily than the cattle (28.6 vs. 1.4%, respectively). In cattle, ground corn is a better energy source than citrus pulp for use by Entodinium and Diplodiniinae. In the buffaloes, the Entodinium are favored by urea and Diplodiniinae species by soybean meal.

Effect of Acute Exposure to Ammonia on Glutamate Transport in Glial Cells Isolated From the Salamander Retina

2001 ◽  
Vol 86 (2) ◽  
pp. 836-844 ◽  
Author(s):  
Dominic Mort ◽  
Païkan Marcaggi ◽  
James Grant ◽  
David Attwell
Keyword(s):  
Glial Cells ◽  
Glutamate Uptake ◽  
Ph Dependence ◽  
Glutamate Transporter ◽  
Ammonia Concentration ◽  
Ammonia Level ◽  
Glutamate Transport ◽  
Acute Exposure ◽  
Ph Change ◽  
Glutamine Synthesis

A rise of brain ammonia level, as occurs in liver failure, initially increases glutamate accumulation in neurons and glial cells. We investigated the effect of acute exposure to ammonia on glutamate transporter currents in whole cell clamped glial cells from the salamander retina. Ammonia potentiated the current evoked by a saturating concentration ofl-glutamate, and decreased the apparent affinity of the transporter for glutamate. The potentiation had a Michaelis-Menten dependence on ammonia concentration, with a K m of 1.4 mM and a maximum potentiation of 31%. Ammonia also potentiated the transporter current produced by d-aspartate. Potentiation of the glutamate transport current was seen even with glutamine synthetase inhibited, so ammonia does not act by speeding glutamine synthesis, contrary to a suggestion in the literature. The potentiation was unchanged in the absence of Cl− ions, showing that it is not an effect on the anion current gated by the glutamate transporter. Ammonium ions were unable to substitute for Na+in driving glutamate transport. Although they can partially substitute for K+ at the cation counter-transport site of the transporter, their occupancy of these sites would produce a potentiation of <1%. Ammonium, and the weak bases methylamine and trimethylamine, increased the intracellular pH by similar amounts, and intracellular alkalinization is known to increase glutamate uptake. Methylamine and trimethylamine potentiated the uptake current by the amount expected from the known pH dependence of uptake, but ammonia gave a potentiation that was larger than could be explained by the pH change, and some potentiation of uptake by ammonia was still seen when the internal pH was 8.8, at which pH further alkalinization does not increase uptake. These data suggest that ammonia speeds glutamate uptake both by increasing cytoplasmic pH and by a separate effect on the glutamate transporter. Approximately two-thirds of the speeding is due to the pH change.

Serial ammonia measurement in patients poisoned with glufosinate ammonium herbicide

2015 ◽  
Vol 35 (5) ◽  
pp. 554-561 ◽  
Author(s):  
JM Moon ◽  
BJ Chun
Keyword(s):  
Odds Ratio ◽  
Independent Predictor ◽  
Ammonia Concentration ◽  
Ammonia Level ◽  
P Value ◽  
Optimal Cutoff ◽  
Cutoff Value ◽  
Glufosinate Ammonium ◽  
Time Latency

This study investigated whether ammonia concentrations can predict delayed neurotoxicity development and neurotoxicity latency in glufosinate ammonium (GLA) herbicide-poisoned patients presenting with an alert mental state and stable hemodynamics. This retrospective observational case study included 26 patients divided into 2 groups: neurotoxicity during hospitalization (complicated group) and without neurotoxicity (noncomplicated group). Thirteen patients (50.0%) experienced neurotoxicity at 16 h post-ingestion. Although ammonia concentrations at presentation did not differ significantly between the two groups, the ammonia level in the complicated group increased significantly at the next measurement and remained significantly higher than that in the noncomplicated group until 48 h after ingestion. The peak ammonia concentration before neurotoxicity development was an independent predictor of neurotoxicity (odds ratio: 1.047, 95% confidence interval: 1.010–1.087, p value = 0.014), and the optimal cutoff value of peak ammonia concentration for predicting neurotoxicity was 101.5 μg/dL. The rate of ammonia increase was not associated with the time latency from ingestion to neurotoxicity development. This study showed that serial ammonia measurements in GLA-poisoned patients may identify those who are at high risk of developing neurotoxicity. However, as this study enrolled few patients, further qualified trials are required to confirm our results and to reveal the etiology of hyperammonemia and its causality in neurotoxicity.

scholarly journals Polyculture of Giant Freshwater Prawn, Macrobrachium rosenbergii and Nilem Carp, Osteochilus hasselti Cultured in Recirculation System Using Biofiltration

2018 ◽  
Vol 47 ◽  
pp. 02005
Author(s):  
Eri Setiadi ◽  
Imam Taufik
Keyword(s):  
Water Quality ◽  
Macrobrachium Rosenbergii ◽  
Stocking Density ◽  
Biomass Productivity ◽  
Freshwater Prawn ◽  
Water Spinach ◽  
Giant Freshwater Prawn ◽  
Recirculation System

Giant Freshwater Prawn is one of the freshwater commodity which have an economy value where Nilem carp has also potentially to be cultured to produce fingerling size as a “baby fish”. The purpose of this experiment is to determine the optimal survival, growth, FCR, and productivity of Giant Freshwater Prawn and Nilem carp cultured on polyculture system and also biomass productivity of bio filter (snail, water spinach, and Cabomba). The different stocking density of Nilem carp as a treatments were as followed: A) 25 fish m-3 B) 50 fish m-3, and C) 75 fish m-3. Density of Giant Freshwater Prawn was 20 individuals m-3 for all treatments. The result showed that the optimal of survival, growth (length and weight), productivity and FCR for Giant Freshwater Prawn was found at density of 25 fish m-3 (A) than that of density 50 fish m-3 (B) and 75 fish m-3 (C) (P<0.05). The highest of biomass productivity of bio filter (snail, water spinach, and Cabomba) was found at density of 25 fish m-3 (A) than that of density of 50 fish m-3 (B) and 75 fish m-3 (P<0.05). Water quality such as DO, temperature and pH showed almost the same at all treatments.

Changes in Ammonia Values of Protein-free Filtrates of Blood During Storage in the Frozen State

1962 ◽  
Vol 8 (6) ◽  
pp. 593-597 ◽  
Author(s):  
Charles E Wilder
Keyword(s):  
Frozen Storage ◽  
Ammonia Concentration ◽  
Ammonia Level ◽  
Blood Ammonia ◽  
Trichloracetic Acid ◽  
Frozen State ◽  
Significant Change

Abstract Protein-free filtrates of blood treated with trichloracetic acid were divided into two or three parts. The ammonia concentration of one of these was determined immediately by the ninhydrin method described by Nathan and Rodkey (1). The others were kept frozen for periods of 1-8 days and then thawed and analyzed. The blood ammonia concentrations calculated from these filtrate values were compared. A significant change in ammonia level during frozen storage was demonstrated.

Glutamine and carbohydrate supplements reduce ammonemia increase during endurance field exercise

2007 ◽  
Vol 32 (6) ◽  
pp. 1186-1190 ◽  
Author(s):  
Jacqueline Carvalho-Peixoto ◽  
Robson Cardilo Alves ◽  
Luiz-Claudio Cameron
Keyword(s):  
Central Fatigue ◽  
Ammonia Concentration ◽  
Ammonia Level ◽  
Strenuous Exercise ◽  
Double Blind Study ◽  
Blood Ammonia ◽  
Double Blind ◽  
Field Situation ◽  
High Level ◽  
The Impact

Blood ammonia concentration increases during endurance exercise and has been proposed as a cause for both peripheral and central fatigue. We examined the impact of glutamine and (or) carbohydrate supplementation on ammonemia in high-level runners. Fifteen men in pre-competitive training ran 120 min (~34 km) outdoors on 4 occasions. On the first day, the 15 athletes ran without the use of supplements and blood samples were taken every 30 min. After that, each day for 4 d before the next 3 exercise trials, we supplemented the athletes’ normal diets in bolus with carbohydrate (1 g·kg–1·d–1), glutamine (70 mg·kg–1·d–1), or a combination of both in a double-blind study. Blood ammonia level was determined before the run and every 30 min during the run. During the control trial ammonia increased progressively to approximately 70% above rest concentration. Following supplementation, independent of treatment, ammonia was not different (p > 0.05) for the first 60 min, but for the second hour it was lower than in the control (p < 0.05). Supplementation in high-level, endurance athletes reduced the accumulation of blood ammonia during prolonged, strenuous exercise in a field situation.

scholarly journals Respiratory Diseases and Hyperammoniemia

Doctor Ru ◽  
2020 ◽  
Vol 19 (11) ◽  
pp. 32-37
Author(s):  
I.G. Bakulin ◽  
◽  
O.Yu. Chizhova ◽  
L.N. Belousova ◽  
E.Yu. Pavlova ◽  
...  
Keyword(s):  
Chronic Bronchitis ◽  
Respiratory Diseases ◽  
Ammonia Concentration ◽  
Ammonia Level ◽  
Nutrition Status ◽  
Exclusion Criterion ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Study Objective ◽  
Study Results

Study Objective: To assess the incidence of hyperammoniemia in respiratory diseases. Materials and Methods. 36 patients with respiratory diseases took part in the study: chronic obstructive pulmonary disease (COPD) (21 (58.3%) patients), chronic bronchitis (5 (13.9%) patients), and pneumonia (10 (27.8%) patients). Mean age was 66.8 ± 11.8 years; 9 (25.0%) females and 27 (75.0%) males. Each patient had his/her capillary ammonia measured (microdiffusion). In order to assess the nutrition status, all patients had their primary somatometry measured: height, weight, body mass index, inactive arm circumference, waist circumference, skin-fat rolls thickness under biceps, above triceps, below shoulder blade angle, in inguinal region. Study Results. 9 (25.0%) out of 36 patients had hyperammoniemia (capillary ammonia level: 74.0 ± 7.1 nmol/L); their mean age was 62.3 ± 18.2 years. Patients with COPD/chronic bronchitis had significantly higher ammonia concentration (60.7 ± 16.6 mol/L) vs. patients with pneumonia (48.4 ± 14.3 mol/L; t = 2.2, p < 0.03). COPD patients demonstrated significant differences in ammonia levels depending on duration of disease. In the group of higher ammonia concentration, COPD lasted significantly longer (t = 4.03 p = 0.001). We did not find any sound correlation between nutritional (trophological) status and ammonia concentrations in patients with respiratory diseases (t < 2, p > 0.05). Conclusion. In 25% of cases, respiratory diseases were associated with hyperammoniemia that is non-cirrhotic, because hepatobiliary disorders in this group of patients were an exclusion criterion. Although no statistically significant correlation between non-cirrhotic hyperammoniemia and nutritional (trophological) status was demonstrated, pathogenic relations between them cannot be ruled out. Whether underweight is a determining factor in non-cirrhotic hyperammoniemia is still unclear and requires further research and more observations. Keywords: ammonia, non-cirrhotic hyperammoniemia, nutritional (trophological) status, respiratory diseases.

Ammonia level as a proxy of asparaginase inactivation in children: A strategy for classification of infusion reactions

2021 ◽  
pp. 107815522199873
Author(s):  
Amanda C Santos ◽  
Marcelo G P Land ◽  
Elisangela C Lima
Keyword(s):  
Clinical Decision Making ◽  
Clinical Symptoms ◽  
Clinical Decision ◽  
Ammonia Concentration ◽  
Ammonia Level ◽  
University Hospital ◽  
First Case ◽  
Infusion Reactions ◽  
Serum Ammonia

Introduction Allergic hypersensitivity reactions related to enzyme asparaginase may occur during intravenous infusion of drugs and other adverse reactions (non-allergic hypersensitivity and hyperammonemia), which do not require discontinuation of therapy as the first case. It makes differential diagnoses between infusion reactions essential to assure the team regarding the right decision to make after the adverse event. This study evaluated a pharmacovigilance strategy of differentiating infusion reactions to asparaginase in pediatric patients, based on the measurement of serum ammonia and the classification of the reactions by clinical symptoms and severity. Methodology We included children, diagnosed with ALL, and treated with native Escherichia coli asparaginase in a university hospital. The professional team monitored and evaluated all asparaginase infusions for continuity of treatment (rechallenge), seeing the measurement of serum ammonia and classification of reactions for type and severity grade. Data from this monitoring was collected retrospectively. Chi-square and Mann-Whitney tests were used to compare the ratios between serum ammonia concentration posterior and before asparaginase infusion. Results 245 infusions in 32 patients were monitored, and 19 reactions were observed in 17 children (53%). Three children have hyperammonemia and continue their treatment. The variation of the serum ammonia levels before and after the infusion was statistically significant, comparing the groups with no reaction or hyperammonemia versus the group with the hypersensitivity reaction. Conclusion The pharmacovigilance strategy applied in the hospital investigated was a useful and inexpensive tool that supported clinical decision-making and enabled the maintenance of asparaginase therapy for three (9,4%) patients followed up.

Sweat ammonia excretion during submaximal cycling exercise

1991 ◽  
Vol 70 (1) ◽  
pp. 371-374 ◽  
Author(s):  
D. Czarnowski ◽  
J. Gorski
Keyword(s):  
Room Temperature ◽  
Ammonia Excretion ◽  
Ammonia Concentration ◽  
Ammonia Level ◽  
Bicycle Ergometer ◽  
Plasma Ammonia ◽  
Cold Room ◽  
Before And After ◽  
Total Ammonia ◽  
The Difference

This study was undertaken to investigate whether part of the ammonia formed during muscular exercise was excreted with the sweat. Male medical students volunteered for the experiment. They exercised 30 min on a bicycle ergometer at 80 and 40% of the predetermined maximal O2 uptake (VO2max). Exercise at 80% VO2max was performed twice, at room temperature (20 degrees C) and in a cold room (0 degrees C), whereas exercise at 40% was performed only at room temperature (20 degrees C). Blood was collected from the antecubital vein immediately before and after exercise. Sweat was collected from the hypogastric region by use of gauze pads. It was shown that the plasma ammonia level was elevated after exercise at 80% VO2max and remained stable after exercise at 40% VO2max. The volume of sweat produced during exercise at 80% VO2max at 20 degrees C was 428 +/- 138 ml and at 0 degrees C 245 +/- 86 ml and during exercise at 40% VO2max was 183 +/- 69 ml. The ammonia concentration in the sweat after exercise at 80% VO2max at 20 degrees C was 7,140 mumol/l and at 0 degrees C 11,816 mumol/l. After exercise at 40% VO2max, it was 2,076 mumol/l. The total ammonia lost through the sweat during exercise at 80% VO2max was similar at both temperatures, despite the difference in the sweat volume (at 20 degrees C, 3,360 +/- 2,080 mumol; at 0 degrees C, 3,310 +/- 1,250 mumol). During exercise at 40% VO2max, it was 350 +/- 230 mumol. These results show that part of ammonia formed during exercise is lost with sweat. The amount lost increases with increased work rate and the plasma ammonia concentration.

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