scholarly journals DUXAP8 a Pan-Cancer Prognostic Marker Involved in the Molecular Regulatory Mechanism in Hepatocellular Carcinoma: A Comprehensive Study Based on Data Mining, Bioinformatics, and in vitro Validation

2019 ◽  
Vol Volume 12 ◽  
pp. 11637-11650 ◽  
Author(s):  
Chaosen Yue ◽  
Chaojie Liang ◽  
Pengyang Li ◽  
Lijun Yan ◽  
Dongxin Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Data Mining ◽  
Prognostic Marker ◽  
Regulatory Mechanism ◽  
Pan Cancer ◽  
Comprehensive Study

scholarly journals Identifying SLC27A5 as a potential prognostic marker of hepatocellular carcinoma by weighted gene co-expression network analysis and in vitro assays

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fan Zhang ◽  
Mengjuan Xue ◽  
Xin Jiang ◽  
Huiyuan Yu ◽  
Yixuan Qiu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Survival Analysis ◽  
Network Analysis ◽  
Prognostic Marker ◽  
In Vitro Assays ◽  
Hub Genes ◽  
Hub Gene ◽  
Tumor Tissues ◽  
Potential Biomarker

Abstract Background The incidence and mortality rates of hepatocellular carcinoma are among the highest of all cancers all over the world. However the survival rates are relatively low due to lack of effective treatments. Efforts to elucidate the mechanisms of HCC and to find novel prognostic markers and therapeutic targets are ongoing. Here we tried to identify prognostic genes of HCC through co-expression network analysis. Methods We conducted weighted gene co-expression network analysis with a microarray dataset GSE14520 of HCC from Gene Expression Omnibus database and identified a hub module associated with HCC prognosis. Function enrichment analysis of the hub module was performed. Clinical information was analyzed to select candidate hub genes. The expression profiles and survival analysis of the selected genes were performed using additional datasets (GSE45267 and TCGA-LIHC) and the hub gene was identified. GSEA and in vitro experiments were conducted to further verify the function of the hub gene. Results Genes in the hub module were mostly involved in the metabolism pathway. Four genes (SLC27A5, SLC10A1, PCK2 and FMO4) from the module were identified as candidate hub genes according to correlation analysis with prognostic indicators. All these genes were significantly down-regulated in tumor tissues compared with non-tumor tissues in additional datasets. After survival analysis and network construction, SLC27A5 was selected as a prognostic marker. GSEA analysis and in vitro assays suggested that SLC27A5 downregulation promoted tumor cell migration via enhancing epithelial-mesenchymal transition. Conclusion SLC27A5 is a potential biomarker of HCC and SLC27A5 downregulation promoted HCC progression by enhancing EMT.

scholarly journals Potential diagnostic and prognostic marker dimethylglycine dehydrogenase (DMGDH) suppresses hepatocellular carcinoma metastasis in vitro and in vivo

Oncotarget ◽  
2016 ◽  
Vol 7 (22) ◽  
pp. 32607-32616 ◽  
Author(s):  
Gang Liu ◽  
Guojun Hou ◽  
Liang Li ◽  
Yixue Li ◽  
Weiping Zhou ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Prognostic Marker ◽  
Carcinoma Metastasis

scholarly journals WASF2 Overexpression and Promoter Hypomethylation Are Associated With Poor Clinical Outcomes in Hepatocellular Carcinoma

2021 ◽  
Author(s):  
Hye Ri Ahn ◽  
Geum Ok Baek ◽  
Moon Gyeong Yoon ◽  
Ju A Son ◽  
Jung Hwan Yoon ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Regulatory Mechanism ◽  
Cpg Island ◽  
Methylation Status ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Hcc Cells

Abstract Background: Hepatocellular carcinoma (HCC) is one of the most common and lethal cancers worldwide. Wiskott-Aldrich syndrome protein family member 2 (WASF2) is an integral member of the actin cytoskeleton pathway that plays a crucial role in cell motility. In this study, we aimed to explore the role of WASF2 in HCC carcinogenesis and its regulatory mechanism. Methods: WASF2 expression in HCC was analyzed using six public RNA-seq datasets and 66 paired tissues from patients with HCC. Role of WASF2 in HCC cell phenotypes was evaluated using small interfering RNA (siRNA) in vitro and in vivo. Epigenetic regulatory mechanism of WASF2 was assessed in the Cancer Genome Atlas liver hepatocellular carcinoma project (TCGA_LIHC) dataset and also validated in 38 paired HCC tissues. Results: WASF2 is overexpressed in HCC and is clinically correlated with prognosis. WASF2 inactivation decreased the viability, growth, proliferation, migration, and invasion of Huh-7 and SNU475 HCC cells by restoring G2/M checkpoint function, inducing cell death, and inhibiting epithelial-mesenchymal transition, and hindering actin polymerization. In addition, WASF2 knockdown using siWASF2 in a xenograft mouse model exerted tumor suppressive effect. Furthermore, we observed a negative correlation between WASF2 methylation status and mRNA expression. The cg24162579 CpG island in the WASF2 5′ promoter region was hypomethylated in HCC compared to matched non-tumor samples. Patients with high WASF2 methylation and low WASF2 expression displayed the highest overall survival.Conclusions: WASF2 is overexpressed and hypomethylated in HCC and correlates with patient prognosis. Moreover, WASF2 inactivation exerts anti-tumorigenic effects on HCC cells in vitro and in vivo, suggesting that WASF2 could be a potential therapeutic target for HCC.

The role of α2, αv and β1 Integrins in cell growth of hepatocellular carcinoma cells in vitro

2018 ◽  
Vol 56 (01) ◽  
pp. E2-E89
Author(s):  
M Juratli ◽  
A Schnitzbauer ◽  
R Blaheta ◽  
W Bechstein
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Growth ◽  
Carcinoma Cells ◽  
Hepatocellular Carcinoma Cells ◽  
Β1 Integrins
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