Molecular Identification of Equine Herpesvirus 1, 2, and 5 in Equids with Signs of Respiratory Disease in Central Ethiopia

Upper respiratory disease has been a serious problem in Standardbred horses on racetracks in Ontario, with outbreaks occurring once or twice annually in late winter and early spring seasons. To determine the causes of these epidemics, a 3-year investigation was carried out in which nasal swabs and serum samples were obtained at intervals from apparently healthy horses and from horses suffering from upper respiratory disease. The nasal swabs were used to isolate bacteria and viruses. The serum samples were examined for the presence and level of antibodies to equine influenza viruses and equine herpesvirus 1. None of the bacteria isolated were associated with the outbreaks of disease. Equine herpesvirus 2 was isolated 72 times from both diseased and apparently healthy horses. Equine herpesvirus 1 was isolated 10 times from horses with respiratory disease, both during and between epidemics. Influenza equine/1 virus was isolated seven times and influenza equine/2 was isolated once during severe outbreaks of upper respiratory disease. Serological evidence confirmed that influenza viruses were the causes of the major epidemics, with the equine/1 strain being involved most often.

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Equine Herpesvirus 1 and 4 Respiratory Disease in the Horse

Clinical Techniques in Equine Practice ◽

10.1053/j.ctep.2006.03.014 ◽

2006 ◽

Vol 5 (3) ◽

pp. 197-202 ◽

Cited By ~ 11

Author(s):

Wendy Harless ◽

Nicola Pusterla

Keyword(s):

Respiratory Disease ◽

Equine Herpesvirus ◽

Equine Herpesvirus 1 ◽

Herpesvirus 1

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Antemortem detection of latent infection with neuropathogenic strains of equine herpesvirus-1 in horses

Journal of the American Veterinary Medical Association ◽

10.2460/javma.229.4.561 ◽

2006 ◽

Vol 229 (4) ◽

pp. 561-561 ◽

Cited By ~ 1

Author(s):

George P. Allen

Keyword(s):

Latent Infection ◽

Equine Herpesvirus ◽

Equine Herpesvirus 1 ◽

Herpesvirus 1

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The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71.

Journal of Virology ◽

10.1128/jvi.70.11.8195-8198.1996 ◽

1996 ◽

Vol 70 (11) ◽

pp. 8195-8198 ◽

Cited By ~ 8

Author(s):

J E Wellington ◽

G P Allen ◽

A A Gooley ◽

D N Love ◽

N H Packer ◽

...

Keyword(s):

Equine Herpesvirus ◽

Equine Herpesvirus 1 ◽

Herpesvirus 1

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Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea

Pathogens ◽

10.3390/pathogens10040425 ◽

2021 ◽

Vol 10 (4) ◽

pp. 425

Author(s):

Hyung-Woo Kang ◽

Eun-Yong Lee ◽

Kyoung-Ki Lee ◽

Mi-Kyeong Ko ◽

Ji-Young Park ◽

...

Keyword(s):

Nucleotide Sequence ◽

South Korea ◽

Molecular Level ◽

Economic Losses ◽

Equine Herpesvirus ◽

Equine Herpesvirus 1 ◽

Mlst Analysis ◽

Herpesvirus 1 ◽

First Time ◽

Group 1

Equine herpesvirus-1 (EHV-1) is an important pathogen in horses. It affects horses worldwide and causes substantial economic losses. In this study, for the first time, we characterized EHV-1 isolates from South Korea at the molecular level. We then aimed to determine the genetic divergences of these isolates by comparing them to sequences in databases. In total, 338 horse samples were collected, and 12 EHV-1 were isolated. We performed ORF30, ORF33, ORF68, and ORF34 genetic analysis and carried out multi-locus sequence typing (MLST) of 12 isolated EHV-1. All isolated viruses were confirmed as non-neuropathogenic type, showing N752 of ORF30 and highly conserved ORF33 (99.7–100%). Isolates were unclassified using ORF68 analysis because of a 118 bp deletion in nucleotide sequence 701–818. Seven EHV-1 isolates (16Q4, 19R166-1, 19R166-6, 19/10/15-2, 19/10/15-4, 19/10/18-2, 19/10/22-1) belonged to group 1, clade 10, based on ORF34 and MLST analysis. The remaining 5 EHV-1 isolates (15Q25-1, 15D59, 16Q5, 16Q40, 18D99) belonged to group 7, clade 6, based on ORF34 and MLST analysis.

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