scholarly journals NS1’ Protein Expression in the JaOArS982 Strain of Japanese Encephalitis Virus Does Not Enhance Virulence in Mice

2015 ◽  
Vol 43 (4) ◽  
pp. 233-237 ◽  
Author(s):  
Yuki Takamatsu ◽  
Muhareva Raekiansyah ◽  
Kouichi Morita ◽  
Daisuke Hayasaka
Keyword(s):  
Protein Expression ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Ns1 Protein

Inhibition of Japanese Encephalitis Virus NS1 Protein Expression in Cell by Small Interfering RNAs

Virus Genes ◽  
2006 ◽  
Vol 33 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Xueqin Liu ◽  
Shengbo Cao ◽  
Rui Zhou ◽  
Gaoyuan Xu ◽  
Shaobo Xiao ◽  
...  
Keyword(s):  
Protein Expression ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
Small Interfering Rnas

scholarly journals NS1′ protein expression facilitates production of Japanese encephalitis virus in avian cells and embryonated chicken eggs

2014 ◽  
Vol 95 (2) ◽  
pp. 373-383 ◽  
Author(s):  
Yuki Takamatsu ◽  
Kenta Okamoto ◽  
Duc Tuan Dinh ◽  
Fuxun Yu ◽  
Daisuke Hayasaka ◽  
...  
Keyword(s):  
Protein Expression ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Mammalian Cells ◽  
Cultured Cells ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
Structural Protein ◽  
Chicken Eggs ◽  
Embryonated Chicken

Japanese encephalitis virus (JEV), which belongs to the genus Flavivirus of the family Flaviviridae, is a leading cause of meningo-encephalitis in Asian countries. The flavivirus non-structural protein 1 (NS1) plays a role in virus replication and in the elicitation of an immune response. The NS1′ protein found among the members of the JEV subgroup is an extended form of NS1 and is generated by a −1 ribosomal frameshift. This protein is known to be involved in viral pathogenicity; however, its specific function is still unknown. Here, we describe an investigation of the molecular function of NS1′ protein through the production of JEV NS1′-expressing and -non-expressing clones and their infection of avian and mammalian cells. Efficient NS1′ protein expression was observed in avian cells and was found to facilitate JEV production in both avian cultured cells and embryonated chicken eggs. NS1′ protein was observed to co-localize with NS5 protein and resulted in increased viral RNA levels in avian cells. These findings clearly indicate that NS1′ enhances the production of JEV in avian cells and may facilitate the amplification/maintenance role of birds in the virus transmission cycle in nature.

Protective immunity of E. coli-synthesized NS1 protein of Japanese encephalitis virus

2007 ◽  
Vol 30 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Cheng-Wen Lin ◽  
Kuang-Ting Liu ◽  
Hong-Da Huang ◽  
Wei-June Chen
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Protective Immunity ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
E Coli

scholarly journals The Japanese Encephalitis Virus NS1′ Protein Inhibits Type I IFN Production by Targeting MAVS

2020 ◽  
Vol 204 (5) ◽  
pp. 1287-1298 ◽  
Author(s):  
Dengyuan Zhou ◽  
Qiuyan Li ◽  
Fan Jia ◽  
Luping Zhang ◽  
Shengfeng Wan ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Type I ◽  
Type I Ifn ◽  
Ns1 Protein

scholarly journals Expression and characterization of recombinant Japanese encephalitis virus NS1 protein in Drosophila S2 cell

2008 ◽  
Vol 2 (Suppl 1) ◽  
pp. P36
Author(s):  
Yize Li ◽  
Marie Flamand ◽  
Dorian Counor ◽  
Nelly Kieffer ◽  
Felix Rey ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Encephalitis Virus ◽  
Ns1 Protein

scholarly journals Japanese Encephalitis Virus NS1′ Protein Interacts with Host CDK1 Protein to Regulate Antiviral Response

2021 ◽  
Author(s):  
Qiuyan Li ◽  
Dengyuan Zhou ◽  
Fan Jia ◽  
Luping Zhang ◽  
Usama Ashraf ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Specific Treatment ◽  
Antiviral Response ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
Cellular Mechanisms

Japanese encephalitis virus (JEV) is the main cause of acute human encephalitis in Asia. The unavailability of specific treatment for Japanese encephalitis demands a better understanding of the basic cellular mechanisms that contribute to the onset of disease.

scholarly journals Discordant Activity of Kaempferol Towards Dengue Virus and Japanese Encephalitis Virus

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1246
Author(s):  
Chit Care ◽  
Wannapa Sornjai ◽  
Janejira Jaratsittisin ◽  
Atitaya Hitakarun ◽  
Nitwara Wikan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Protein Expression ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Denv Infection ◽  
Significant Inhibition ◽  
Encephalitis Virus ◽  
Host Protein ◽  
Cell Protein ◽  
Infected Cells

Kaempferol, a plant-derived flavonoid, has been reported to have activity against Japanese encephalitis virus (JEV) in BHK-21 cells. To determine the broader utility of this compound, we initially evaluated the activity of kaempferol against JEV and dengue virus (DENV) in HEK293T/17 cells. Results showed no significant antiviral activity against either virus. We subsequently investigated the activity of kaempferol against both JEV and DENV in BHK-21 cells. Results showed a significant inhibition of JEV infection but, surprisingly, a significant enhancement of DENV infection. The effect of kaempferol on both host protein expression and transcription was investigated and both transcriptional and translational inhibitory effects were observed, although a more marked effect was observed on host cell protein expression. Markedly, while GRP78 was increased in DENV infected cells treated with kaempferol, it was not increased in JEV infected cells treated with kaempferol. These results show that cellular alteration induced by one compound can have opposite effects on viruses from the same family, suggesting the presence of distinct replication strategies for these two viruses.

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