Optimization of the Cultivation Processof a Producer Clone of the Recombinant Lysosomal Iduronate-2-sulfatase Enzyme

This paper describes experiments aimed at developing conditions for suspension culturing of a producer clone of the recombinant lysosomal enzyme iduronate-2-sulfatase based on the CHO cell line. As a result of sequential culturing in batch and fed-batch modes, the optimal nutrient medium composition was established, providing a 2.5-fold increase in the iduronate-2-sulfatase specific activity. Optimization of the feed formulation was carried out, which increased the yield of recombinant iduronate-2-sulfatase. The ambr® 15 Cell Culture System of minibioreactors was used to optimize the cultivation process. Chinese hamster ovary cells, CHO, iduronate-2-sulfatase, idursulfase, lysosomal enzyme, sulfatases, Аmbr Tap Biosystems, ambr® 15 Cell Culture System, mucopolysaccharidosis type II, formyl glycine generating enzyme, optimization of cell culturing conditions, fed-batch culture, batch culture.

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Autophagy and apoptosis of recombinant Chinese hamster ovary cells during fed-batch culture: Effect of nutrient supplementation

Biotechnology and Bioengineering ◽

10.1002/bit.23165 ◽

2011 ◽

Vol 108 (9) ◽

pp. 2182-2192 ◽

Cited By ~ 23

Author(s):

Young Kue Han ◽

Tae Kwang Ha ◽

So Jeong Lee ◽

Jae Seong Lee ◽

Gyun Min Lee

Keyword(s):

Batch Culture ◽

Chinese Hamster Ovary ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Fed Batch ◽

Nutrient Supplementation ◽

Ovary Cells ◽

Fed Batch Culture

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Development of a fed-batch culture process for enhanced production of recombinant human antithrombin by Chinese hamster ovary cells

Journal of Bioscience and Bioengineering ◽

10.1263/jbb.100.502 ◽

2005 ◽

Vol 100 (5) ◽

pp. 502-510 ◽

Cited By ~ 35

Author(s):

Shinobu Kuwae ◽

Toyoo Ohda ◽

Hiroshi Tamashima ◽

Hideo Miki ◽

Kaoru Kobayashi

Keyword(s):

Batch Culture ◽

Chinese Hamster Ovary ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Fed Batch ◽

Ovary Cells ◽

Enhanced Production ◽

Culture Process ◽

Fed Batch Culture

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Cell cycle-specific activity of type I and type II cyclic adenosine 3':5'-monophosphate-dependent protein kinases in Chinese hamster ovary cells.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)33439-7 ◽

1976 ◽

Vol 251 (11) ◽

pp. 3313-3319

Author(s):

M Costa ◽

E W Gerner ◽

D H Russell

Keyword(s):

Cell Cycle ◽

Protein Kinases ◽

Chinese Hamster Ovary ◽

Specific Activity ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Type I ◽

Type Ii ◽

Ovary Cells ◽

Dependent Protein

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In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

Turkish Journal of Biochemistry ◽

10.1515/tjb-2020-0082 ◽

2020 ◽

Vol 45 (5) ◽

pp. 631-637

Author(s):

Cansu Ozel-Tasci ◽

Gozde Pilatin ◽

Ozgur Edeer ◽

Sukru Gulec

Keyword(s):

Cell Culture ◽

Culture System ◽

Metabolic Diseases ◽

Enzymatic Digestion ◽

Cell Culture System ◽

Culture Studies ◽

Experimental Approaches ◽

In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

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