scholarly journals On the Use of a TiO2 Sol-Gel for Horseradish Peroxidase Enzyme Immobilization for Development of an Amperometric Biosensor

2021 ◽  
Author(s):  
Carolina da Rocha ◽  
Carolina Uliana ◽  
Hideko Yamanaka
Keyword(s):  
Horseradish Peroxidase ◽  
Sol Gel ◽  
Pharmaceutical Formulations ◽  
Dip Coating ◽  
Pencil Graphite Electrode ◽  
Aminosalicylic Acid ◽  
Electron Mediator ◽  
Tio2 Sol ◽  
Gel Film ◽  
Peroxidase Enzyme

The sol-gel technique has drawn considerable attention in the area of biomolecules immobilization by virtue of its intrinsic properties including chemical inertness, thermal stability, and good biocompatibility. The present work reports the development of a bio-sensing methodology which involves the immobilization of horseradish peroxidase enzyme (HRP) on pencil graphite electrode (PGE) using TiO2 sol-gel and its application for 5-aminosalicylic acid (5-ASA) determination in pharmaceutical formulations. TiO2 sol-gel film was deposited on the PGE surface by dip coating, and the HRP enzyme was immobilized on the film by adsorption and cross-linked binding using glutaraldehyde. The analytical performance of the methodology was investigated through amperometric measurements at –0.08 V in the presence of 5-ASA (electron mediator) and hydrogen peroxide (substrate). The biosensor was successfully applied for 5-ASA determination, where limits of detection and quantification of 3.3 and 10.0 μmol L-1, respectively, were obtained. The application of the methodology for 5-ASA determination in pharmaceutical formulations led to satisfactory recovery rates ranging from 80 to 98%. The biosensor developed in this work is simple, inexpensive and has the potential to be applied for the determination of other pharmaceutical compounds, which also act as electron mediators in the catalytic cycle of HRP.

Changes of thickness and refractive index of silica sol-gel film by dip coating process

2014 ◽  
Vol 26 (7) ◽  
pp. 72005
Author(s):  
张日东 Zhang Ridong ◽  
严鸿维 Yan Hongwei ◽  
吕海兵 Lü Haibing ◽  
张尽力 Zhang Jinli ◽  
晏良宏 Yan Lianghong ◽  
...  
Keyword(s):  
Refractive Index ◽  
Sol Gel ◽  
Dip Coating ◽  
Silica Sol ◽  
Coating Process ◽  
Gel Film

The Influencing Factors for the Thickness of Silica Sol-Gel Film by the Dip Coating Process

2014 ◽  
Vol 597 ◽  
pp. 165-169
Author(s):  
Jie Hong Lei ◽  
Ri Dong Zhang
Keyword(s):  
Thin Films ◽  
Surface Roughness ◽  
Refractive Index ◽  
Sol Gel ◽  
Dip Coating ◽  
Silica Sol ◽  
Coating Process ◽  
Power Functions ◽  
Withdrawal Speed ◽  
Gel Film

The porous sol-gel silica thin films were prepared on silicon substrate by the dip coating process. The surface roughness and microstructure of the film was measured by optical microscopy and AFM .Coating thickness and refractive index were measured by ellipsometry method. Influence of withdrawal speed and concentration of colloid was investigated for the thickness of silica. The relation of the thickness and withdrawal speed was fitted by the linear and power functions, and the results were analyzed and compared. It was found that the films with the same thickness prepared by different concentrations of colloid have different refractive indexes. The experimental results indicated that the thickness and refractive index of the film can be controlled by changing the withdrawal speed and colloid concentration.

(Photo)Electrochemical characterization of nanoporous TiO2 and Ce-doped TiO2 sol–gel film electrodes

2012 ◽  
Vol 83 ◽  
pp. 113-124 ◽  
Author(s):  
Sutasinee Kityakarn ◽  
Yingyot Pooarporn ◽  
Prayoon Songsiriritthigul ◽  
Attera Worayingyong ◽  
Simone Robl ◽  
...  
Keyword(s):  
Sol Gel ◽  
Electrochemical Characterization ◽  
Doped Tio2 ◽  
Tio2 Sol ◽  
Gel Film

Application of Nanometer TiO2 Photocatalysis Material in Air Purification

2007 ◽  
Author(s):  
Haiyan Hou ◽  
Dongsheng Zhu ◽  
Jun Cheng
Keyword(s):  
Sol Gel ◽  
Dip Coating ◽  
Woven Fabric ◽  
Air Purification ◽  
Tio2 Photocatalysis ◽  
Composite Support ◽  
Tio2 Sol ◽  
Optimum Formulation ◽  
Oil Mist ◽  
Dip Coating Technique

The production of oil mist in machinery processing workshop is harmful. To control concentration of oil mist, the TiO2 which can treat with manifold organic pollutants is used to purify oil mist. At first, the nanometer TiO2 is prepared using the sol-gel method on the optimum formulation that showed a higher activity. Then it is treated by dip-coating technique using the non-woven fabric as composite support. The efficiency of suppression of oil mist is compared with ordinary materials. With the increase of time, the purification of the nanometer TiO2 photocatalyst sieve against oil mist of non-woven fabric is investigated in solar radiation at room temperature. It oxidizes pollutants of oil mist to CO2 and H2O. The experiments demonstrat oil mist is oxidized on the TiO2 sol at the 22nd minute with a maximum capacity of 0.3219g. It infers from the experiments the photodegradation effect is high and the reaction is fast. It also concludes that the nanometer TiO2 photocatalysis material is an ideal material for suppressing oil mist in air. At last, a status the application of technology in air purification as well as its problems and trends are presented. The technique has a promising prospect to solve the increasing air problem of the workshop.

Study of Improvement Technology of Photocatalytic Activity on the TiO2 Sol by Plasma Treatment After Dip-Coating Processing

2003 ◽  
Vol 10 (04) ◽  
pp. 635-640 ◽  
Author(s):  
C.-K. Jung ◽  
S.-H. Cho ◽  
S.-B. Lee ◽  
T.-K. Kim ◽  
M.-N. Lee ◽  
...  
Keyword(s):  
Thin Films ◽  
Photocatalytic Activity ◽  
Plasma Treatment ◽  
Sol Gel ◽  
Catalytic Performance ◽  
Dip Coating ◽  
Rf Plasma ◽  
Tio2 Sol ◽  
Afm Analysis ◽  
Gel Processing

We have deposited titanium dioxide TiO 2 thin films on glass using a single molecular precursor such as titanium (IV) isopropoxide ( Ti [ OCH ( CH 3)2]4, 97%) by sol–gel processing. Argon and oxygen rf plasma treatments at 295 K for 0.5 h in the power range of 50–200 W were also used to elevate photocatalytic activity of the as-grown TiO 2 films. A superhydrophilic property and surface morphology change appeared in the light irradiation with O 2 plasma treatment. In this work, the effect of the plasma with photocatalyst ( TiO 2) on the improvement of hydrophilic properties has mainly been investigated. Photocatalytic activity was evaluated by measurements of the uv/vis. irradiation, refractive index, contact angle, and AFM analysis. We confirmed that plasma treatment is a very reliable method for the synthesis of TiO 2 thin films with high catalytic performance.

scholarly journals Raman Spectroscopy and XRD investigation on TiO2 sol-gel dip coating thin films synthesizes with and without solvents

2018 ◽  
Vol 150 ◽  
pp. 04007 ◽  
Author(s):  
Shuhadah A. Yazid ◽  
Zulkifli Mohd. Rosli ◽  
Jariah Mohamad Juoi ◽  
Nur Dalilah Johari
Keyword(s):  
Thin Films ◽  
Raman Spectroscopy ◽  
Crystallite Size ◽  
Sol Gel ◽  
Dip Coating ◽  
X Ray Diffraction ◽  
Coating Films ◽  
Tio2 Sol ◽  
Coating Method ◽  
Dip Coating Method

TiO2 coating films were prepared by sol-gel dip coating method using TTiP precursors with and without ethanol as a medium to hydrolyze the HCl catalyst. The prepared samples of titanium dioxide (TiO2) coating were dried at 110°C for 30 minutes then heated at 500°C and 600°C for 1h and 3h respectively to study effect of annealing temperature and soaking time. The crystallinity of the TiO2 coating were identified by using X-ray Diffraction technique and crystallite size was calculated. Raman Spectroscopy also used as the confirmation of phases formation of TiO2 coating films. Crystallite size of TiO2 films were obtained at 12.35 nm, 17.29 nm for anatase, 21.71 nm, 28.95 nm for rutile and 2.19 nm for brookite. Thus, the establishment of the desired phases, crystallinity and grain size of TiO2 thin films sol-gel can be controlled and encouraging to explore as an effort toward producing a sustainable photocatalytic.

Modification of Electroless Nickel-Plating Coatings with Sol-Gel Film

2005 ◽  
Vol 475-479 ◽  
pp. 3835-3838 ◽  
Author(s):  
Hui Cong Liu ◽  
Liqun Zhu ◽  
Yan Bin Du
Keyword(s):  
Heat Treatment ◽  
Oxidation Resistance ◽  
Sol Gel ◽  
Electroless Nickel ◽  
Dip Coating ◽  
Treatment Temperature ◽  
Gel Film ◽  
Sol Gel Films ◽  
Corrosion And Oxidation

Four Sol-Gel films were developed by dip-coating electroless nickel-plated samples in different Sols, followed by heat treatment. The effects of the coating times and heat treatment temperature on the corrosion, oxidation resistance and wearing quality of the modified coating were examined. Results show that, after six coating, Sol-Gel films obtained can provide good corrosion and oxidation resistance, and their anti-corrosion quality remains high even after being oxidized at 500 °C for 60 hours. Addition of inorganic molybdenum salt or chromate into the Ti Sol also makes the wearing quality of the Sol-Gel film better.

Spectral hole burning in sol-gel-derived Eu3+-doped film

2000 ◽  
Vol 15 (5) ◽  
pp. 1190-1194 ◽  
Author(s):  
Masayuki Nogami ◽  
Tomotaka Ishikawa ◽  
Tomokatsu Hayakawa ◽  
Tomokatsu Hayakawa
Keyword(s):  
Sol Gel ◽  
Dip Coating ◽  
Treatment Temperature ◽  
Hole Burning ◽  
Spectral Hole Burning ◽  
Spectral Hole ◽  
Gel Film ◽  
Total Fluorescence ◽  
Gel Technique ◽  
The Eu

The sol-gel technique was applied to the preparation of Eu3+ ion–doped films, which showed persistent spectral hole burning. A gel film of ∼3500-nm thickness was prepared by dip-coating of the solution synthesized from Si(OC2H5)4, CH3Si(OC2H5)3, Eu(NO3)3 · 6H2O, and hydropropyl-cellulose. The spectral hole was burned in the 7F0 → 5D0 transition band of the Eu3+ ions at 7 K, the depth of which was 24% of the total fluorescence intensity and decreased as the heat-treatment temperature of film increased. It was found that the hole was thermally filled and erased above ∼170 K; the temperature at which the hole was erased was lower for the film heated at high temperature.

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