scholarly journals Variability and genetic diversity among Streptomyces scabiei infected potato cultivars with common scab from Egypt

2019 ◽  
Vol 0 (0) ◽  
pp. 57-72
Author(s):  
M. Hussein ◽  
Mona Hassan ◽  
A. Elfarash
Keyword(s):  
Genetic Diversity ◽  
Common Scab ◽  
Potato Cultivars ◽  
Streptomyces Scabiei

scholarly journals Investigation of Streptomyces scabies Causing Potato Scab by Various Detection Techniques, Its Pathogenicity and Determination of Host-Disease Resistance in Potato Germplasm

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 760
Author(s):  
Sohaib Ismail ◽  
Bo Jiang ◽  
Zohreh Nasimi ◽  
M. Inam-ul-Haq ◽  
Naoki Yamamoto ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Common Scab ◽  
Streptomyces Scabies ◽  
Potato Cultivars ◽  
Potato Tubers ◽  
Streptomyces Species ◽  
Detection Techniques ◽  
Streptomyces Spp ◽  
Geographical Regions

Streptomyces scabies is a Gram-positive bacterial pathogen that causes common scab disease to several crops, particularly in the potato. It is a soil borne pathogen, a very devastating scab pathogen and difficult to manage in the field. Streptomyces has several species that cause common scab such as S. scabiei, S. acidiscabies, S. europaeiscabiei, S. luridiscabiei, S. niveiscabiei, S. puniciscabiei, S. reticuliscabiei, S. stelliscabiei, S. turgidiscabies, S. ipomoeae. Common scab disease harmfully affects potato economic and market value due to the presence of black spots on the tuber. Owing to its genetic diversity and pathogenicity, the determination of pathogen presence in potato fields is still challenging. In this study, S. scabies genetic diversity was measured by surveying five potato-growing areas of Pakistan during the growing season 2019. A total of 50 Streptomyces isolates, including S. scabies, S. acidiscabies, S. griseoflavus were isolated and identified based on morphologic, biochemical and molecular analysis. Virulent confirmation assays confirmed ten virulent strains of Streptomyces spp. On the potato cultivars Cardinal and Santee. Among the Streptomyces species, S. scabies showed the highest scab index, followed by S. acidiscabies and S. griseoflavus by exhibiting the scab-like lesions on potato tubers. Ten potato cultivars were screened against these virulent isolates of Streptomyces. The Faisalabad white variety showed the highest scab index followed By Cardinal, Tourag, Kuroda, Santee, Lady Rosetta, Asterix, Diamant, Faisalabad red and Sadaf. Moreover, genetic diversity and pathogenicity of Streptomyces spp. on potato tubers were also likely diverse in different geographical regions and also potato cultivars. This study represents a contribution to understanding the local interaction between potatoes and Streptomyces spp. in Pakistan. It will aid in supporting a solution for the management of this pathogen around the world.

Genetic diversity of Meloidogyne spp. parasitising potato in Brazil and aggressiveness of M. javanica populations on susceptible cultivars

Nematology ◽  
2017 ◽  
Vol 19 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Israel L. Medina ◽  
Cesar B. Gomes ◽  
Valdir R. Correa ◽  
Vanessa S. Mattos ◽  
Philippe Castagnone-Sereno ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Potato Production ◽  
Potato Cultivars ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Nematode Reproduction ◽  
Meloidogyne Spp ◽  
Prevalent Species ◽  
Reproduction Factor

Root-knot nematodes (Meloidogyne spp.) significantly impact potato production worldwide and in Brazil they are considered one of the most important group of nematodes affecting potatoes. The objectives of this study were to survey Meloidogyne spp. associated with potatoes in Brazil, determine their genetic diversity and assess the aggressiveness of M. javanica on two susceptible potato cultivars. Fifty-seven root-knot nematode populations were identified using esterase phenotyping, including Meloidogyne javanica, M. incognita, M. arenaria and M. ethiopica. Overall, root-knot nematodes were present in ca 43% of sampled sites, in which M. javanica was the most prevalent species, and the phenotypes Est J3, J2a and J2 occurred in 91.2, 6.7 and 2.1% of the positive samples, respectively. Other species, such as M. incognita, M. arenaria and M. ethiopica, were found less frequently and occurred at rates of 6.4, 4.3 and 2.1% of the samples, respectively. Sometimes, M. javanica was found in mixtures with other root-knot nematodes in ca 10.6% of sites containing Meloidogyne. After confirming the identification of 17 isolates of M. javanica and one isolate each of M. incognita, M. arenaria and M. ethiopica by SCAR markers, the populations were used to infer their genetic diversity using RAPD markers. Results revealed low intraspecifc genetic diversity among isolates (13.9%) for M. javanica. Similarly, M. javanica sub-populations (J2a) clustered together (81% of bootstrap), indicating subtle variation from typical J3 populations. The aggressiveness of four populations of M. javanica from different Brazilian states on two susceptible potato cultivars was tested under glasshouse conditions. Results indicated differences in aggressiveness among these populations and showed that potato disease was proportional to nematode reproduction factor.

scholarly journals Recommendation for the conservation of the name Streptomyces scabies. Request for an Opinion

2007 ◽  
Vol 57 (10) ◽  
pp. 2447-2448 ◽  
Author(s):  
D. H. Lambert ◽  
R. Loria ◽  
D. P. Labeda ◽  
G. S. Saddler
Keyword(s):  
Common Scab ◽  
Streptomyces Scabies ◽  
General Recognition ◽  
Streptomyces Scabiei

The primary streptomycete inciting common scab of potato was first legitimately described by Thaxter in 1892 as ‘Oospora scabies’, preserving the spelling of an epithet in use since 1846. The name Streptomyces scabies, dating to 1948, was revived in 1989, but changed to Streptomyces scabiei in 1997 to follow grammatical convention. Considering the long-established use and general recognition of ‘scabies’, it is proposed that the original epithet be conserved.

Screening potato cultivars for common scab of potato in a naturally infested field

2001 ◽  
Vol 44 (1) ◽  
pp. 19-24 ◽  
Author(s):  
K. K. Mishra ◽  
J. S. Srivastava
Keyword(s):  
Common Scab ◽  
Potato Cultivars ◽  
Infested Field

Assessment of a glasshouse test for measuring the resistance of potato cultivars to common scab

1985 ◽  
Vol 28 (3) ◽  
pp. 379-387 ◽  
Author(s):  
P. D. S. Caligari ◽  
R. L. Wastie
Keyword(s):  
Common Scab ◽  
Potato Cultivars

scholarly journals On Selection of Resistant Potato Cultivars to Common Scab(S. scabies)

2004 ◽  
Vol 10 (2) ◽  
pp. 135-137 ◽  
Author(s):  
Soon-Yeong Hong ◽  
Yong-Kil Kang ◽  
Young-Il Hahm
Keyword(s):  
Common Scab ◽  
Potato Cultivars ◽  
Selection Of

scholarly journals Genetic Diversity of Potato Cultivars for Nitrogen Use Efficiency Under Contrasting Nitrogen Regimes

2019 ◽  
Author(s):  
Baye Berihun Getahun ◽  
Molla Mekonen Kassie ◽  
Richard G. F. Visser ◽  
C. Gerard van der Linden
Keyword(s):  
Genetic Diversity ◽  
Nitrogen Use Efficiency ◽  
Potato Cultivars ◽  
Nitrogen Use ◽  
Use Efficiency

Detection of potential suberinase-encoding genes in Streptomyces scabiei strains and other actinobacteria

2013 ◽  
Vol 59 (5) ◽  
pp. 294-303 ◽  
Author(s):  
Doaa Komeil ◽  
Anne-Marie Simao-Beaunoir ◽  
Carole Beaulieu
Keyword(s):  
Esterase Activity ◽  
Common Scab ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Open Reading Frames ◽  
Streptomyces Scabiei ◽  
Polymerase Chain ◽  
Encoding Genes ◽  
Reading Frames ◽  
Important Disease

Streptomyces scabiei causes common scab, an economically important disease of potato tubers. Some authors have previously suggested that S. scabiei penetration into host plant tissue is facilitated by secretion of esterase enzymes degrading suberin, a lipidic biopolymer of the potato periderm. In the present study, S. scabiei EF-35 showed high esterase activity in suberin-containing media. This strain also exhibited esterase activity in the presence of other biopolymers, such as lignin, cutin, or xylan, but at a much lower level. In an attempt to identify the esterases involved in suberin degradation, translated open reading frames of S. scabiei 87-22 were examined for the presence of protein sequences corresponding to extracellular esterases of S. scabiei FL1 and of the fungus Coprinopsis cinerea VTT D-041011, which have previously been shown to be produced in the presence of suberin. Two putative extracellular suberinase genes, estA and sub1, were identified. The presence of these genes in several actinobacteria was investigated by Southern blot hybridization, and both genes were found in most common-scab-inducing strains. Moreover, reverse transcription – polymerase chain reaction performed with S. scabiei EF-35 showed that estA was expressed in the presence of various biopolymers, including suberin, whereas the sub1 gene appeared to be specifically expressed in the presence of suberin and cutin.

Responses of potato cultivars to the common scab pathogens, Streptomyces scabies and S. turgidiscabies

2005 ◽  
Vol 146 (3) ◽  
pp. 395-403 ◽  
Author(s):  
L H HILTUNEN ◽  
A WECKMAN ◽  
A YLHAINEN ◽  
H RITA ◽  
E RICHTER ◽  
...  
Keyword(s):  
Common Scab ◽  
Streptomyces Scabies ◽  
Potato Cultivars ◽  
The Common

scholarly journals Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

2010 ◽  
Vol 10 (3) ◽  
pp. 204-210 ◽  
Author(s):  
Elizângela Almeida Rocha ◽  
Luciano Vilela Paiva ◽  
Humberto Henrique de Carvalho ◽  
Claudia Teixeira Guimarães
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Ssr Markers ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Potato Cultivars ◽  
High Information Content ◽  
Commercial Potato ◽  
Polymorphic Bands ◽  
High Information

This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.

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