scholarly journals Entomopathogenic Fungus as A Biocontrol Agent Against The Cattle Tick Boophilus annulatus (Say) (Acari: Ixodidae)

2007 ◽  
Vol 1 (1) ◽  
pp. 45-47
Author(s):  
Kawther El-Kammah ◽  
Laila Oyoun
2020 ◽  
Vol 23 (2) ◽  
pp. 1-6
Author(s):  
Helben Ismat Mohammaed ◽  
Mohammed Saeed Mirza ◽  
Feyroz Ramadan Hassan

Beauveria bassiana is an important entomopathogenic fungus that used as a biocontrol agent of insect pests. Maintaining and preserving B. bassiana cultures is essential for the effective evaluation of its potential as microbial agent against insect pest, for biodiversity studies and also for exchange of fungal material between laboratories. In the present work we evaluated the suitability of four preservation materials based on gelatin and rice to maintain the viability of B. bassiana to be used as baits for insect’s control. The gelatin amended with sugar recorded the maximum viability after 70 days of storage as 98.3% compared to 63.3% on rice media. Effective control of 80% of the ants was observed after 10 days of feeding on gelatin amended with sugar and rice bait. The results demonstrated that treatment with formulations containing conidia of B. bassiana presents insecticidal activity against ant in addition of acting as preservation materials.


1964 ◽  
Vol 54 (4) ◽  
pp. 633-641 ◽  
Author(s):  
R. J. Thorpe ◽  
P. Walker

A field study was made of the effects of γ BHC, Sevin, toxaphene and ronnel (fenchlorphos) used as sprays on the numbers of ticks infesting cattle at Shika stockfarm in the Northern Guinea Savannah zone of Nigeria.The tick population included Boophilus annulatus (Say), B. decoloratus (Koch), Amblyomma variegatum (F.), Hyalomma truncatum Koch and Rhipiccphalus simus simus Koch, and its distribution on the host is described.Significance tests of treatment difference were carried out for initial kill and reinfestation rate. In both dry- and wet-season trials toxaphene proved to be the most effective ixodicide. A satisfactory degree of control was given by γ BHC but not by Sevin or ronnel.It is concluded that spraying at 21- and 7-day intervals during the dry and wet seasons, respectively, with either toxaphene or γ BHC will maintain a stable, satisfactory level of tick parasitism and premunity to tick-borne diseases in the herd.


2003 ◽  
Vol 56 ◽  
pp. 118-122
Author(s):  
R.J. Townsend ◽  
M. O'Callaghan ◽  
V.W. Johnson ◽  
T.A. Jackson

Microbial control agents targeting soildwelling organisms need to be compatible with commonly used fertilisers The bacterium Serratia entomophila is used as a microbial control agent for control of the New Zealand grass grub Costelytra zealandica and Beauveria bassiana is an entomopathogenic fungus used to control a range of insect pests These biocontrol agents were formulated into granules and applied to pots together with five fertilisers commonly used on pastures throughout New Zealand Compatibility with S entomophila was also assessed in a field trial where treatments were applied by direct drilling and surface application There appeared to be no deleterious effect from the application of the fertiliser treatments on the establishment and survival of either S entomophila or B bassiana On the contrary there was a suggestion that some nitrogenous fertilisers may lead to an increase in numbers of the bacterial biocontrol agent


2013 ◽  
Vol 66 ◽  
pp. 379-379
Author(s):  
P.W. Shaw ◽  
D.R. Wallis ◽  
T.L. Nelson ◽  
T.A. Jackson

Fullers rose weevil (FRW Naupactus cervinus) is a troublesome pest in pipfruit orchards since adult weevils often lay their eggs in irrigation sprinkler heads causing blockages throughout the season Adult weevils feed mainly on apple foliage and can damage and lay eggs on fruit The entomopathogenic fungus Beauveria bassiana was assessed as a biocontrol agent against Fullers rose weevil in a field trial at a Nelson pipfruit orchard with a large FRW population Larvae of the pest live in the soil and are susceptible to strains of the fungus The trial consisted of five randomised treated and untreated threetree apple plots (10 m2) A granular formulation of the fungus was applied by hand (50 g/m2) in May 2012 and irrigated into the soil Soil samples were collected in September 2012 and analysis showed the fungus had established in treated plots In treated plots average numbers of larvae were reduced by 70 and in four of the five plots emerging adults were reduced by an average of 80 These preliminary results indicate the potential for a granular formulation of Beauveria bassiana to be used as a broadcast application against FRW in orchards


Author(s):  
Shawky Mohamed Aboelhadid ◽  
Samar Mahmoud Ibrahium ◽  
Walid Mahmoud Arafa ◽  
Lilian Nagy Maahrous ◽  
Abdel-Azeem Shaban Abdel-Baki ◽  
...  

Plant Disease ◽  
2018 ◽  
Vol 102 (5) ◽  
pp. 977-982 ◽  
Author(s):  
Manzoor Hussain ◽  
Miloslav Zouhar ◽  
Pavel Ryšánek

The entomopathogenic fungus Lecanicillium muscarium (Petch) Zare and Gams is currently being developed as a biocontrol agent against insect pests, as well as some plant-pathogenic fungi and bacteria. Data about its activity against plant-parasitic nematodes exist, but are relatively limited. To expand this understanding, we investigated the biocontrol efficiency of three isolates of L. muscarium (Lm) against the root knot nematode, Meloidogyne incognita, in both in vitro and in vivo conditions. In our experiments, the maximum number of nematode eggs, juveniles (J2s), females, and egg masses that were parasitized were quantified after a 72-h exposure to the fungus. The isolate Lm1 was designated as the best biocontrol agent against nematode eggs as well as J2s. It showed the highest colonization of eggs and significantly decreased egg hatching events. The results from two additional isolates, Lm2 and Lm3, were also significant (P = 0.05) but less pronounced than those observed with Lm1. L. muscarium treatments had significant (P = 0.05) positive effects on plant shoot and root growth compared with the growth of control plants. These results suggest the effectiveness of the fungus may be due to either the infection of eggs and J2s, or the production of secondary metabolites that induced plant defense mechanisms and lead to systemic resistance. Our study demonstrates that L. muscarium could be used as a potential biocontrol agent against root knot nematodes.


2016 ◽  
Vol 9 (2) ◽  
pp. 77 ◽  
Author(s):  
Yadi Suryadi ◽  
Tri P. Priyatno ◽  
I Made Samudra ◽  
Dwi N. Susilowati ◽  
Nuni Lawati ◽  
...  

<p>Beauveria bassiana is one of the<br />entomopathogenic fungus that produces chitinase when<br />infecting its host. This study was aimed to purify, isolate and<br />characterize chitinase of B. bassiana isolate BB200109.<br />Pathogen identity was determined both morphologically and<br />molecularly using ITS primer, whilst characterization was<br />done at various conditions i.e. temperature, pH, metal ion<br />and incubation time. Results showed that the BB200109<br />isolate belonged to B. bassiana. The isolate produced<br />extracellular chitinase with chitinolytic index of 1.035. Partial<br />purification of three saturated ammonium sulphate<br />precipitation (10, 30, and 70%) showed maximum purity of<br />1.2 times, while dialysis could increase the purity of 1.9<br />times compared to that of crude enzyme extract.<br />Characterization results showed that the chitinase isolated<br />from B. bassiana isolate BB200109 had an optimum activity<br />at pH 4, temperature 50oC, and optimum incubation time of<br />90 minutes. The effect of metal ions (60 mM) Mn2+ served as<br />activator, while EDTA, K+, Mg2+, Cu2+, Fe2+, Zn2+, and Na+<br />acted as inhibitors. The chitinase demonstrated lower<br />affinity to chitin substrate as indicated by high Km value of<br />0.266 mg/l and a Vmax of 0.067 mg/l sec. Based on SDS-PAGE,<br />chitinase from B. bassiana isolate BB200109 had molecular<br />weight of 60.25 kDa. The study implied the potency of<br />B. bassiana isolate BB200109 as extracellular chitinase<br />producer with its enzyme charateristics seems to be<br />developed as an insect biocontrol agent.</p>


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