scholarly journals Pemurnian Parsial dan Karakterisasi Kitinase Asal Jamur Entomopatogen Beauveria bassiana Isolat BB200109

2016 ◽  
Vol 9 (2) ◽  
pp. 77 ◽  
Author(s):  
Yadi Suryadi ◽  
Tri P. Priyatno ◽  
I Made Samudra ◽  
Dwi N. Susilowati ◽  
Nuni Lawati ◽  
...  
Keyword(s):  
Metal Ions ◽  
Beauveria Bassiana ◽  
Incubation Time ◽  
Entomopathogenic Fungus ◽  
Metal Ion ◽  
Biocontrol Agent ◽  
Enzyme Extract ◽  
Crude Enzyme Extract ◽  
Sds Page ◽  
Km Value

<p>Beauveria bassiana is one of the<br />entomopathogenic fungus that produces chitinase when<br />infecting its host. This study was aimed to purify, isolate and<br />characterize chitinase of B. bassiana isolate BB200109.<br />Pathogen identity was determined both morphologically and<br />molecularly using ITS primer, whilst characterization was<br />done at various conditions i.e. temperature, pH, metal ion<br />and incubation time. Results showed that the BB200109<br />isolate belonged to B. bassiana. The isolate produced<br />extracellular chitinase with chitinolytic index of 1.035. Partial<br />purification of three saturated ammonium sulphate<br />precipitation (10, 30, and 70%) showed maximum purity of<br />1.2 times, while dialysis could increase the purity of 1.9<br />times compared to that of crude enzyme extract.<br />Characterization results showed that the chitinase isolated<br />from B. bassiana isolate BB200109 had an optimum activity<br />at pH 4, temperature 50oC, and optimum incubation time of<br />90 minutes. The effect of metal ions (60 mM) Mn2+ served as<br />activator, while EDTA, K+, Mg2+, Cu2+, Fe2+, Zn2+, and Na+<br />acted as inhibitors. The chitinase demonstrated lower<br />affinity to chitin substrate as indicated by high Km value of<br />0.266 mg/l and a Vmax of 0.067 mg/l sec. Based on SDS-PAGE,<br />chitinase from B. bassiana isolate BB200109 had molecular<br />weight of 60.25 kDa. The study implied the potency of<br />B. bassiana isolate BB200109 as extracellular chitinase<br />producer with its enzyme charateristics seems to be<br />developed as an insect biocontrol agent.</p>

scholarly journals Preservative Media for Beauveria Bassiana (bals.) Vuill Storage

2020 ◽  
Vol 23 (2) ◽  
pp. 1-6
Author(s):  
Helben Ismat Mohammaed ◽  
Mohammed Saeed Mirza ◽  
Feyroz Ramadan Hassan
Keyword(s):  
Beauveria Bassiana ◽  
Insecticidal Activity ◽  
Entomopathogenic Fungus ◽  
Biocontrol Agent ◽  
Insect Pests ◽  
Insect Pest ◽  
Effective Control ◽  
Effective Evaluation ◽  
Microbial Agent

Beauveria bassiana is an important entomopathogenic fungus that used as a biocontrol agent of insect pests. Maintaining and preserving B. bassiana cultures is essential for the effective evaluation of its potential as microbial agent against insect pest, for biodiversity studies and also for exchange of fungal material between laboratories. In the present work we evaluated the suitability of four preservation materials based on gelatin and rice to maintain the viability of B. bassiana to be used as baits for insect’s control. The gelatin amended with sugar recorded the maximum viability after 70 days of storage as 98.3% compared to 63.3% on rice media. Effective control of 80% of the ants was observed after 10 days of feeding on gelatin amended with sugar and rice bait. The results demonstrated that treatment with formulations containing conidia of B. bassiana presents insecticidal activity against ant in addition of acting as preservation materials.

scholarly journals Compatibility of microbial control agents Serratia entomophila and Beauveria bassiana with selected fertilisers

2003 ◽  
Vol 56 ◽  
pp. 118-122
Author(s):  
R.J. Townsend ◽  
M. O'Callaghan ◽  
V.W. Johnson ◽  
T.A. Jackson
Keyword(s):  
New Zealand ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Deleterious Effect ◽  
Biocontrol Agent ◽  
Insect Pests ◽  
Microbial Control ◽  
Control Agent ◽  
Costelytra Zealandica ◽  
Direct Drilling

Microbial control agents targeting soildwelling organisms need to be compatible with commonly used fertilisers The bacterium Serratia entomophila is used as a microbial control agent for control of the New Zealand grass grub Costelytra zealandica and Beauveria bassiana is an entomopathogenic fungus used to control a range of insect pests These biocontrol agents were formulated into granules and applied to pots together with five fertilisers commonly used on pastures throughout New Zealand Compatibility with S entomophila was also assessed in a field trial where treatments were applied by direct drilling and surface application There appeared to be no deleterious effect from the application of the fertiliser treatments on the establishment and survival of either S entomophila or B bassiana On the contrary there was a suggestion that some nitrogenous fertilisers may lead to an increase in numbers of the bacterial biocontrol agent

scholarly journals Investigating the potential of Beauveria bassiana as a biocontrol agent for Fullers rose weevil (Naupactus cervinus)

2013 ◽  
Vol 66 ◽  
pp. 379-379
Author(s):  
P.W. Shaw ◽  
D.R. Wallis ◽  
T.L. Nelson ◽  
T.A. Jackson
Keyword(s):  
Emerging Adults ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Field Trial ◽  
Biocontrol Agent ◽  
Soil Samples ◽  
Preliminary Results ◽  
Broadcast Application ◽  
Fungus Beauveria Bassiana

Fullers rose weevil (FRW Naupactus cervinus) is a troublesome pest in pipfruit orchards since adult weevils often lay their eggs in irrigation sprinkler heads causing blockages throughout the season Adult weevils feed mainly on apple foliage and can damage and lay eggs on fruit The entomopathogenic fungus Beauveria bassiana was assessed as a biocontrol agent against Fullers rose weevil in a field trial at a Nelson pipfruit orchard with a large FRW population Larvae of the pest live in the soil and are susceptible to strains of the fungus The trial consisted of five randomised treated and untreated threetree apple plots (10 m2) A granular formulation of the fungus was applied by hand (50 g/m2) in May 2012 and irrigated into the soil Soil samples were collected in September 2012 and analysis showed the fungus had established in treated plots In treated plots average numbers of larvae were reduced by 70 and in four of the five plots emerging adults were reduced by an average of 80 These preliminary results indicate the potential for a granular formulation of Beauveria bassiana to be used as a broadcast application against FRW in orchards

scholarly journals Preservative Media for Beauveria Bassiana (bals.) Vuill Storage

2020 ◽  
Vol 23 (2) ◽  
pp. 1-6
Author(s):  
Helben Ismat Mohammaed ◽  
Mohammed Saeed Mirza ◽  
Feyroz Ramadan Hassan
Keyword(s):  
Beauveria Bassiana ◽  
Insecticidal Activity ◽  
Entomopathogenic Fungus ◽  
Biocontrol Agent ◽  
Insect Pests ◽  
Insect Pest ◽  
Effective Control ◽  
Effective Evaluation ◽  
Microbial Agent

Beauveria bassiana is an important entomopathogenic fungus that used as a biocontrol agent of insect pests. Maintaining and preserving B. bassiana cultures is essential for the effective evaluation of its potential as microbial agent against insect pest, for biodiversity studies and also for exchange of fungal material between laboratories. In the present work we evaluated the suitability of four preservation materials based on gelatin and rice to maintain the viability of B. bassiana to be used as baits for insect’s control. The gelatin amended with sugar recorded the maximum viability after 70 days of storage as 98.3% compared to 63.3% on rice media. Effective control of 80% of the ants was observed after 10 days of feeding on gelatin amended with sugar and rice bait. The results demonstrated that treatment with formulations containing conidia of B. bassiana presents insecticidal activity against ant in addition of acting as preservation materials.

scholarly journals Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp

2018 ◽  
Vol 16 (2) ◽  
pp. 173
Author(s):  
Sumardi Sumardi ◽  
Rochmah Agustrina ◽  
Bambang Irawan ◽  
Indah Selfiana
Keyword(s):  
Magnetic Field ◽  
Metal Ions ◽  
Incubation Time ◽  
Metal Ion ◽  
Culture Medium ◽  
Protease Production ◽  
Bacillus Sp ◽  
A Value ◽  
The Magnetic Field ◽  
Time Culture

ABSTRAKTujuan penelitian ini adalah mengetahui pengaruh paparan medan magnet 0.2 mT pada ion logam Fe dan Zn dalam media pertumbuhan terhadap produksi protease Bacillus sp.  Ion logam yang digunakan yaitu Fe dan Zn dalam bentuk garam FeCl3 dan ZnCl2. Hasil penelitian menunjukkan bahwa ion logam Fe (0.01% b/v) yang terpapar medan magnet   mempunyai nilai Indeks Proteolitik 3,36 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,74 dengan lama inkubasi 18 jam. Sedang ion logam Fe (0.01% b/v) yang tidak terpapar medan magnet mempunyai nilai Indeks Proteolitik 1,79 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,12 dengan lama inkubasi 18 jam. Sedangkan pada ion logam Zn (0.005% b/v) yang terpapar medan magnet dan tidak terpapar medan magnet menyebabkan aktivitas Bacillus mati. Larutan ion logam Fe 0,01% dalam media cair menghasilkan produksi protease lebih baik (0,06 U/ml) dibandingkan dengan produksi protease tanpa dipemapar medan magnet (0,00 U/ml).Kata kunci: Bacillus sp., paparan medan magnet, indeks proteolitik.ABSTRACTThe purpose of this study was to determine the effect of metal ions Fe and Zn were exposed to a magnetic field of 0.2 mT to the production of proteases in Bacillus sp. The metal ions used are Fe and Zn in the form of FeCl3 and ZnCl2 salts. The results showed that the culture medium containing metal ions Fe (a 0.01% w/v) were exposed by the magnetic field has a value of proteolytic index of 3.36 with 10 hour long incubation, and proteolysis index of 2.74 with 18 hour incubation time. Culture medium containing metal ions Fe (a 0.01% w/v) which are not being exposed to the magnetic field has a value of proteolytic index of 1.79 with 10-hour long incubation, and proteolysis index of 2.12 with 18 hour incubation time. While the culture medium containing metal ions Zn (0.005% w/v) either exposed or not exposed to the magnetic field causes the activity of Bacillus sp die. The solution of 0.01% Fe metal ion in liquid medium resulted in better protease production (0.06 U/ml) than production of proteases without exposure to magnetic fields (0.00  U/ml)Keywords: Bacillus sp., magnetic field, proteolytic index, and proteolysis indexSitasi: Sumardi, Agustrina R., Irawan B., dan I. Selfiana. (2018). Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp. Jurnal Ilmu Lingkungan, 16(2), 173-177,doi:10.14710/jil.16.2.173-177

Use of agro-waste (Musa paradisiaca peels) as a sustainable biosorbent for toxic metal ions removal from contaminated water

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Contact Time ◽  
Metal Ion ◽  
Equilibrium Concentration ◽  
Ion Concentration ◽  
Contaminated Water ◽  
Musa Paradisiaca ◽  
Percentage Removal ◽  
Adsorbent Dose

A study of removal of heavy metal ions from heavy metal contaminated water using agro-waste was carried out with Musa paradisiaca peels as test adsorbent. The study was carried by adding known quantities of lead (II) ions and cadmium (II) ions each and respectively into specific volume of water and adding specific dose of the test adsorbent into the heavy metal ion solution, and the mixture was agitated for a specific period of time and then the concentration of the metal ion remaining in the solution was determined with Perkin Elmer Atomic absorption spectrophotometer model 2380. The effect of contact time, initial adsorbate concentration, adsorbent dose, pH and temperature were considered. From the effect of contact time results equilibrium concentration was established at 60minutes. The percentage removal of these metal ions studied, were all above 90%. Adsorption and percentage removal of Pb2+ and Cd2+ from their aqueous solutions were affected by change in initial metal ion concentration, adsorbent dose pH and temperature. Adsorption isotherm studies confirmed the adsorption of the metal ions on the test adsorbent with good mathematical fits into Langmuir and Freundlich adsorption isotherms. Regression correlation (R2) values of the isotherm plots are all positive (&gt;0.9), which suggests too, that the adsorption fitted into the isotherms considered.

The Binding and Viscometric Studies of Ni+2, Co+2 and Mn+2 Ions with Protein by Spectrometric and pH Metric Techniques

2019 ◽  
Vol 9 (2) ◽  
pp. 151-162
Author(s):  
Shveta Acharya ◽  
Arun Kumar Sharma
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Protein Molecule ◽  
Vital Role ◽  
Sufficient Evidence ◽  
Structural Requirement ◽  
Nickel Ions ◽  
Cobalt Ions ◽  
Lower Temperature ◽  
Specific Oxidation

Background: The metal ions play a vital role in a large number of widely differing biological processes. Some of these processes are quite specific in their metal ion requirements. In that only certain metal ions, in specific oxidation states, can full fill the necessary catalytic or structural requirement, while other processes are much less specific. Objective: In this paper we report the binding of Mn (II), Ni (II) and Co (II) with albumin are reported employing spectrophotometric and pH metric method. In order to distinguish between ionic and colloidal linking, the binding of metal by using pH metric and viscometric methods and the result are discussed in terms of electrovalent and coordinate bonding. Methods: The binding of Ni+2, Co+2 and Mn+2 ions have been studied with egg protein at different pH values and temperatures by the spectrometric technique. Results: The binding data were found to be pH and temperature dependent. The intrinsic association constants (k) and the number of binding sites (n) were calculated from Scatchard plots and found to be at the maximum at lower pH and at lower temperatures. Therefore, a lower temperature and lower pH offered more sites in the protein molecule for interaction with these metal ions. Statistical effects seem to be more significant at lower Ni+2, Co+2 and Mn+2 ions concentrations, while at higher concentrations electrostatic effects and heterogeneity of sites are more significant. Conclusion: The pH metric as well as viscometric data provided sufficient evidence about the linking of cobalt, nickel and manganese ions with the nitrogen groups of albumin. From the nature and height of curves in the three cases it may be concluded that nickel ions bound strongly while the cobalt ions bound weakly.

Efficient Preconcentration of Cu2+, Zn2+ and Fe3+ with an Agarose-Schiff Base Sorbent

2007 ◽  
Vol 72 (7) ◽  
pp. 908-916 ◽  
Author(s):  
Payman Hashemi ◽  
Hatam Hassanvand ◽  
Hossain Naeimi
Keyword(s):  
Schiff Base ◽  
Metal Ions ◽  
Metal Ion ◽  
Good Accuracy ◽  
Kinetic Studies ◽  
Sample Volume ◽  
Effects Of Ph ◽  
Glass Column ◽  
High Concentrations ◽  
Ph Range

Sorption and preconcentration of Cu2+, Zn2+ and Fe3+ on a salen-type Schiff base, 2,2'- [ethane-1,2-diylbis(nitrilomethylidyne)]bis(2-methylphenol), chemically immobilized on a highly crosslinked agarose support, were studied. Kinetic studies showed higher sorption rates of Cu2+ and Fe3+ in comparison with Zn2+. Half-times (t1/2) of 31, 106 and 58 s were obtained for sorption of Cu2+, Zn2+ and Fe3+ by the sorbent, respectively. Effects of pH, eluent concentration and volume, ionic strength, buffer concentration, sample volume and interferences on the recovery of the metal ions were investigated. A 5-ml portion of 0.4 M HCl solution was sufficient for quantitative elution of the metal ions from 0.5 ml of the sorbent packed in a 6.5 mm i.d. glass column. Quantitative recoveries were obtained in a pH range 5.5-6.5 for all the analytes. The volumes to be concentrated exceeding 500 ml, ionic strengths as high as 0.5 mol l-1, and acetate buffer concentrations up to 0.3 mol l-1 for Zn2+ and 0.4 mol l-1 for Cu2+ and Fe3+ did not have any significant effect on the recoveries. The system tolerated relatively high concentrations of diverse ions. Preconcentration factors up to 100 and detection limits of 0.31, 0.16 and 1.73 μg l-1 were obtained for Cu2+, Zn2+ and Fe3+, respectively, for their determination by a flame AAS instrument. The method was successfully applied to the metal ion determinations in several river water samples with good accuracy.

Urobiliverdin, a New Bile Pigment Deriving from Uroporphyrin

1983 ◽  
Vol 38 (9-10) ◽  
pp. 753-757 ◽  
Author(s):  
Eva Benedikt ◽  
Hans-Peter Köst
Keyword(s):  
Carboxylic Acid ◽  
Main Product ◽  
Aminolevulinic Acid ◽  
Chromic Acid ◽  
Bile Pigment ◽  
Enzyme Extract ◽  
Crude Enzyme Extract ◽  
Chromatographic Behaviour ◽  
Vis Spectroscopy ◽  
Rhodopseudomonas Spheroides

5-Aminolevulinic acid is incubated with a crude enzyme extract from Rhodopseudomonas spheroides, mutant R 26. The formed porphyrins (main product: uroporphyrin III) are isolated. Incorporation of iron, ring-splitting by coupled oxydation and subsequent iron removal leads to a mixture of pigments, from which urobiliverdin, a new bile pigment with eight carboxylic acid side chains, is isolated. It is characterized by its chromatographic behaviour, chromic acid degra­dation and UV-vis spectroscopy.

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