scholarly journals COMPARISON OF THE EFFECTS OF FOETAL BOVINE CALF SERUM WITH THOSE OF BOTH CAMEL AND RABBIT SERUM ON SOME TYPES OF TISSUE CULTURE

2019 ◽  
Vol 65 (163) ◽  
pp. 98-105
Keyword(s):  
Tissue Culture ◽  
Calf Serum ◽  
Rabbit Serum ◽  
Bovine Calf Serum

AN INVESTIGATION OF THE PATHOGENESIS OF HASHIMOTO'S DISEASE BY THYROID TISSUE CULTURE

1960 ◽  
Vol 20 (2) ◽  
pp. 83-NP ◽  
Author(s):  
W. J. IRVINE
Keyword(s):  
Tissue Culture ◽  
Alternative Hypothesis ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Rabbit Serum ◽  
Thyroid Cell ◽  
Thyroid Extract ◽  
Thyroid Cells ◽  
Hashimoto’S Disease ◽  
Hashimoto's Disease

SUMMARY Human thyroid cells were grown in tissue culture in media containing normal human serum, Hashimoto serum, and rabbit sera containing antibodies to purified human thyroglobulin and to crude thyroid extract, respectively. The thyroid cells grew equally well in all media, with the exception of the rabbit serum containing antibodies to crude thyroid extract. Intact thyroid cells obtained from tissue culture failed to fix Hashimoto antibodies in the presence of complement, whereas the constituents of disrupted thyroid cells gave a strongly positive complement-fixation test with Hashimoto serum. It is therefore suggested that the intact thyroid cell is impermeable to complement-fixing Hashimoto antibody. The evidence afforded by the present work adds further weight to the belief that Hashimoto's disease may not be due to a simple auto-immunizing process consequent upon the interaction of thyroid antigen and the known circulating auto-antibodies. Evidence in support of an alternative hypothesis involving 'cell-bound' antibodies with disruption of the follicular basement membrane is discussed.

Effects of Resin or Charcoal Treatment on Fetal Bovine Serum and Bovine Calf Serum

2009 ◽  
Vol 34 (4) ◽  
pp. 101-108 ◽  
Author(s):  
Zhimin Cao ◽  
Clint West ◽  
Carol S. Norton-Wenzel ◽  
Robert Rej ◽  
Faith B. Davis ◽  
...  
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Calf Serum ◽  
Bovine Calf Serum ◽  
Fetal Bovine

In vitro friction and lubrication of large bearing hip prostheses

2009 ◽  
Vol 224 (7) ◽  
pp. 853-864 ◽  
Author(s):  
S Flanagan ◽  
E Jones ◽  
C Birkinshaw
Keyword(s):  
Large Diameter ◽  
Calf Serum ◽  
Mixed Lubrication ◽  
Joint Stability ◽  
Bovine Calf Serum ◽  
Reinforced Polymer ◽  
Metal On Metal ◽  
Different Diameters ◽  
Friction And Lubrication

New material combinations and designs of artificial hip implants are being introduced in an effort to improve proprioception and functional longevity. Larger joints in particular are being developed to improve joint stability, and it is thought that these larger implants will be more satisfactory for younger and more physically active patients. The study detailed here used a hip friction simulator to assess the friction and lubrication properties of large-diameter hip bearings of metal-on-metal and ceramic-on-reinforced-polymer couplings. Joints of different diameters were evaluated to determine what effect, if any, bearing diameter had on lubrication. In addition, the effects of lubricant type are considered, using carboxymethyl cellulose and bovine calf serum, and the physiological lubricant is shown to be considerably more effective at reducing friction. The frictional studies showed that the metal-on-metal joints worked under a mixed lubrication regime, producing similar friction factor values to each other. The addition of bovine calf serum (BCS) reduced the friction. The ceramic-on-reinforced-polymer samples were shown to operate with high friction factors and mixed lubrication. When tested with BCS, the larger-diameter bearings showed a decrease in friction compared with the smaller-size bearings, and the addition of BCS resulted in an increase in friction, unlike the metal-on-metal system. The study demonstrated that the component's diameter had little or no influence on the lubrication and friction of the large bearing combinations tested.

scholarly journals THE IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES

1965 ◽  
Vol 121 (5) ◽  
pp. 835-848 ◽  
Author(s):  
Zanvil A. Cohn ◽  
Belinda Benson
Keyword(s):  
Acid Phosphatase ◽  
Serum Concentration ◽  
Hydrolytic Enzyme ◽  
Calf Serum ◽  
Hydrolytic Enzymes ◽  
Biochemical Properties ◽  
Mononuclear Phagocytes ◽  
Rabbit Serum ◽  
Granule Formation

The concentration of newborn calf serum in the medium has marked effects on the morphological and biochemical properties of mouse mononuclear phagocytes. At a low serum concentration, the cells developed small numbers of tiny cytoplasmic granules and little or no increase in acid phosphatase, cathepsin, and ß-glucuronidase. As the serum concentration was raised, granules were formed at a more rapid rate and were larger in size. The rate of production and total amount of three hydrolytic enzymes was increased at higher levels of serum. Observations on living cells indicated that the phase-dense granules which accumulated in the perinuclear region were derived from pinocytic vesicles. These clear vesicles fused and migrated to the centrosphere where they underwent a gradual increase in phase density and reacted positively for acid phosphatase. A microscopic technique was described for the evaluation of the pinocytic process. When this method was employed, the rate of pinocytosis increased curvilinearly with elevations in the calf serum concentration of the medium. The comparative influence of bovine, horse, and rabbit serum on mouse cells was evaluated. It is suggested that pinocytosis is a major regulator of granule formation and hydrolytic enzyme production by the mouse macrophage.

A micro tissue culture test for the titration and neutralization of rubella virus

1969 ◽  
Vol 15 (1) ◽  
pp. 67-71 ◽  
Author(s):  
John Furesz ◽  
Pierre Moreau ◽  
Walter Yarosh
Keyword(s):  
Tissue Culture ◽  
Rubella Virus ◽  
Fetal Calf Serum ◽  
Constant Pressure ◽  
Calf Serum ◽  
Rabbit Kidney ◽  
Tissue Cultures ◽  
Virus Titration ◽  
One Step ◽  
Microneutralization Test

A simple and reproducible micro tissue culture assay has been devised in RK13 and LLC-RK1 rabbit kidney cells for the titration and neutralization of rubella virus. In this "one-step" assay all virus and serum dilutions were prepared with spiral loops in disposable microplates and tissue cultures suspended in medium 199 and 3% horse or fetal calf serum were added to the microcups simultaneously. Micro tissue cultures were kept in a humidified incubator (36 °C) under a constant pressure of 5% CO2 for 8 days and were read microscopically for viral cytopathic changes on the seventh and eighth day. The microneutralization test performed in LLC-RK1 cell cultures was shown to be a reliable method for the detection of small amounts of rubella antibodies in human sera.The micro assay may be also applied to the virus titration of live, attenuated rubella vaccines.

INSULIN RELEASE FROM HUMAN FOETAL PANCREAS IN TISSUE CULTURE

1973 ◽  
Vol 59 (1) ◽  
pp. 65-79 ◽  
Author(s):  
F. N. LEACH ◽  
M. A. ASHWORTH ◽  
A. J. BARSON ◽  
R. D. G. MILNER
Keyword(s):  
Tissue Culture ◽  
Insulin Release ◽  
Culture Medium ◽  
Calf Serum ◽  
Insulin Degradation ◽  
Optimal Conditions ◽  
Plasma Clot ◽  
Collagenase Digestion ◽  
Incubation Experiments ◽  
Foetal Pancreas

SUMMARY Various methods of tissue culture were studied in an attempt to grow human foetal pancreas under conditions favourable for insulin release. Simple dicing of pancreas was superior to plasma clot adhesion or collagenase digestion for the preparation of tissue for culture. The culture medium described by Kahri (1966) (containing 25% heated postnatal calf serum) was the most suitable of four tested for the study of insulin release from the tissue culture. In this medium insulin could be measured quantitatively by radioimmunoassay and insulin degradation occurred at the rate of 20–25%/24 h. In other media the pancreas grew less well or insulin degradation was much greater. Human foetal pancreas grown under optimal conditions released insulin for up to 34 days. Insulin released into the culture medium did not appear to inhibit the further release of insulin. In some experiments the total amount of insulin released into the culture medium was several-fold greater than that in the pancreas originally seeded. In acute incubation experiments barium and theophylline stimulated insulin release from pancreas cultures. It proved impossible to identify the cells from which insulin was released but they did not appear to be in the monolayer which was composed of fibroblasts.

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