Infectivity of Chlamydia trachomatis in tissue culture with newborn calf serum.

1982 ◽  
Vol 15 (5) ◽  
pp. 951-953 ◽  
Author(s):  
L J LaScolea ◽  
S M Baldigo
Keyword(s):  
Tissue Culture ◽  
Chlamydia Trachomatis ◽  
Calf Serum ◽  
Newborn Calf Serum

scholarly journals LOSS OF IRON FROM MOUSE PERITONEAL MACROPHAGES IN VITRO AFTER UPTAKE OF [55FE]FERRITIN AND [55FE]FERRITIN RABBIT ANTIFERRITIN COMPLEXES

1974 ◽  
Vol 62 (3) ◽  
pp. 802-814 ◽  
Author(s):  
Martha E. Fedorko
Keyword(s):  
Tissue Culture ◽  
Recovery Period ◽  
Calf Serum ◽  
Petri Dish ◽  
Peritoneal Macrophages ◽  
Newborn Calf Serum ◽  
Antibody Complex ◽  
Mouse Peritoneal Macrophages ◽  
Counting Cell

Mouse peritoneal macrophages in culture for 24 h were exposed to horse [55Fe]ferritin and rabbit antihorse [55Fe]ferritin antibody complex and the amount of 55Fe in the medium was assayed up to 2 days after the pulse uptake. Cell survival was assayed by photographing the same areas of the tissue culture Petri dish on successive days and by counting cell numbers per unit area. In experiments in which quantitative assay for cell death is negligible, about 10–20% of the iron ingested by pinocytosis or phagocytosis is released to iron-free medium containing either freshly dialyzed or deironized newborn calf serum (10%). Over the 2-day postpulse period, iron loss is linear. This loss of iron to the medium is significantly reduced by adding iron-saturated newborn calf serum in the postpulse recovery period. A significant portion of the iron released to the medium is bound to transferrin. When human serum is used in the tissue culture system, similar quantities (10–25%) of the ingested iron are lost to the medium 2 days after the pulse.

Dexamethasone-induced differentiation of atrial myocytes in culture

1992 ◽  
Vol 263 (3) ◽  
pp. H722-H729 ◽  
Author(s):  
T. M. Muir ◽  
J. Hair ◽  
G. C. Inglis ◽  
J. W. Dow ◽  
G. B. Lindop ◽  
...  
Keyword(s):  
Culture Medium ◽  
Structural Integrity ◽  
Ventricular Myocytes ◽  
Light And Electron Microscopy ◽  
Calf Serum ◽  
Atrial Myocytes ◽  
Newborn Calf Serum ◽  
Fetal Rats ◽  
Storage Granules ◽  
Specific Mrna

Atrial and ventricular myocytes from fetal and newborn rats were cultured in medium supplemented with fetal or newborn calf serum with and without glucocorticoid. Myocyte morphology was examined by light and electron microscopy, and the amount of stored and secreted atrial natriuretic peptide (ANP) was measured. Without dexamethasone, neonatal atrial myocytes cultured for 7 days contained myofibrils organized into sarcomeres and numerous endocrine granules containing immunostainable ANP. Secretion of immunoreactive ANP reached a peak between days 7 and 9 of culture. Myocytes from fetal rats secreted ANP but contained few endocrine granules, and myofilaments were poorly organized. By contrast, the addition of dexamethasone (1 nM-1 microM) to the culture medium of newborn myocytes promoted development of numerous endocrine storage granules, mitochondria, and myofibrils with prominent Z-bands. Dexamethasone also increased the cellular content of ANP and ANP-specific mRNA in both atrial and ventricular myocytes. In the presence of dexamethasone myocytes maintained their structural integrity for periods of at least 45 days.

Oesophagostomum radiatum: inhibition of in vitro development by newborn calf serum

1990 ◽  
Vol 64 (1) ◽  
pp. 9-14
Author(s):  
I. J. East ◽  
C. J. Fitzgerald
Keyword(s):  
Inhibitory Action ◽  
Natural Infection ◽  
Calf Serum ◽  
Foetal Calf Serum ◽  
Newborn Calf Serum ◽  
In Vitro Development ◽  
Specific Antibodies ◽  
Serum Inhibition ◽  
Vitro Development

ABSTRACTOesophagostomum radiatum developed to fourth stage larvae after 14 days in in vitro culture. However, development was totally inhibited if the standard 50% foetal calf serum in the medium was replaced by newborn calf serum. Inhibition did not occur with serum from cattle immune to O. radiatum through natural infection or experimental vaccination irrespective of the titre of specific antibodies to O. radiatum in each serum. The inhibitory action of NCS could be abolished by heat treatment at 56°C for 1 h but not by dialysis or repeated freeze-thawing. The inhibition was not consistent with observed differences in the activity of 19 enzymes in the various sera or the absence of various thiol-containing stimulants of worm development.

A micro tissue culture test for the titration and neutralization of rubella virus

1969 ◽  
Vol 15 (1) ◽  
pp. 67-71 ◽  
Author(s):  
John Furesz ◽  
Pierre Moreau ◽  
Walter Yarosh
Keyword(s):  
Tissue Culture ◽  
Rubella Virus ◽  
Fetal Calf Serum ◽  
Constant Pressure ◽  
Calf Serum ◽  
Rabbit Kidney ◽  
Tissue Cultures ◽  
Virus Titration ◽  
One Step ◽  
Microneutralization Test

A simple and reproducible micro tissue culture assay has been devised in RK13 and LLC-RK1 rabbit kidney cells for the titration and neutralization of rubella virus. In this "one-step" assay all virus and serum dilutions were prepared with spiral loops in disposable microplates and tissue cultures suspended in medium 199 and 3% horse or fetal calf serum were added to the microcups simultaneously. Micro tissue cultures were kept in a humidified incubator (36 °C) under a constant pressure of 5% CO2 for 8 days and were read microscopically for viral cytopathic changes on the seventh and eighth day. The microneutralization test performed in LLC-RK1 cell cultures was shown to be a reliable method for the detection of small amounts of rubella antibodies in human sera.The micro assay may be also applied to the virus titration of live, attenuated rubella vaccines.

INSULIN RELEASE FROM HUMAN FOETAL PANCREAS IN TISSUE CULTURE

1973 ◽  
Vol 59 (1) ◽  
pp. 65-79 ◽  
Author(s):  
F. N. LEACH ◽  
M. A. ASHWORTH ◽  
A. J. BARSON ◽  
R. D. G. MILNER
Keyword(s):  
Tissue Culture ◽  
Insulin Release ◽  
Culture Medium ◽  
Calf Serum ◽  
Insulin Degradation ◽  
Optimal Conditions ◽  
Plasma Clot ◽  
Collagenase Digestion ◽  
Incubation Experiments ◽  
Foetal Pancreas

SUMMARY Various methods of tissue culture were studied in an attempt to grow human foetal pancreas under conditions favourable for insulin release. Simple dicing of pancreas was superior to plasma clot adhesion or collagenase digestion for the preparation of tissue for culture. The culture medium described by Kahri (1966) (containing 25% heated postnatal calf serum) was the most suitable of four tested for the study of insulin release from the tissue culture. In this medium insulin could be measured quantitatively by radioimmunoassay and insulin degradation occurred at the rate of 20–25%/24 h. In other media the pancreas grew less well or insulin degradation was much greater. Human foetal pancreas grown under optimal conditions released insulin for up to 34 days. Insulin released into the culture medium did not appear to inhibit the further release of insulin. In some experiments the total amount of insulin released into the culture medium was several-fold greater than that in the pancreas originally seeded. In acute incubation experiments barium and theophylline stimulated insulin release from pancreas cultures. It proved impossible to identify the cells from which insulin was released but they did not appear to be in the monolayer which was composed of fibroblasts.

survival of bovine embryos frozen in media supplemented with newborn calf serum or bovine serum albumin

1984 ◽  
Vol 21 (1) ◽  
pp. 223 ◽  
Author(s):  
K.R. Bondioli ◽  
C.B. Brunson ◽  
C.R. Looney ◽  
J.M. Massey ◽  
A.B. McGrath ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Calf Serum ◽  
Bovine Embryos ◽  
Newborn Calf Serum

scholarly journals Optimization of Conditions for In Vitro Culture of the Microphallid DigeneanGynaecotyla adunca

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Jenna West ◽  
Alexandra Mitchell ◽  
Oscar J. Pung
Keyword(s):  
Egg Production ◽  
Horse Serum ◽  
Calf Serum ◽  
Culture Conditions ◽  
In Vitro Cultivation ◽  
Ilyanassa Obsoleta ◽  
Uca Pugnax ◽  
Newborn Calf Serum ◽  
Chicken Serum

In vitro cultivation of digeneans would aid the development of effective treatments and studies of the biology of the parasites. The goal of this study was to optimize culture conditions for the trematode,Gynaecotyla adunca. Metacercariae of the parasite from fiddler crabs,Uca pugnax, excysted in trypsin, were incubated overnight to permit fertilization, and were cultured in different conditions to find those that resulted in maximum worm longevity and egg production. When cultured in media lacking serum, worms lived longer in Hanks balanced salt solution and Dulbecco’s Modified Eagle medium/F-12 (DME/F-12) than in RPMI-1640 but produced the most eggs in DME/F-12. Worm longevity and egg production increased when worms were grown in DME/F-12 supplemented with 20% chicken, horse, or newborn calf serum but the greatest number of eggs was deposited in cultures containing horse or chicken serum. Horse serum was chosen over chicken serum due to the formation of a precipitate in chicken serum. The optimal concentration of horse serum with respect to egg production ranged from 5 to 20%. Infectivity of eggs deposited by worms in culture was tested by feeding eggs to mud snails,Ilyanassa obsoleta. None of these snails producedG. aduncacercariae.

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