scholarly journals Human Dental Pulp Tissue during Orthodontic Tooth Movement: An Immunofluorescence Study

2020 ◽  
Vol 5 (3) ◽  
pp. 65 ◽  
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Giovanni Matarese ◽  
Angela Militi ◽  
Marco Matarese ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Pulp Tissue ◽  
Force Application ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp

The orthodontic tooth movement is the last step of several biological processes that take place after the application of external forces. During this process, dental pulp tissue is subjected to structural and protein expression modifications in order to maintain their integrity and functional morphology. The purpose of the present work was to perform an in vivo study, evaluating protein expression modifications in the human dental pulp of patients that have undergone orthodontic tooth movement due to pre-calibrated light force application for 30 days. Dental pulp samples were extracted from molars and premolars of the control group and after 7 and 30 days of treatment; the samples were then processed for immunofluorescence reactions using antibodies against fibronectin, collagen I and vascular endothelial growth factor (VEGF). Our results show that, after 7 days of treatment, all tested proteins change their pattern expression and will reset after 30 days. These data demonstrate that the dental pulp does not involve any irreversible iatrogenic alterations, supporting the efficacy and safety of using pre-calibrated force application to induce orthodontic tooth movement in clinical practice.

scholarly journals Age-related changes of dental pulp tissue after experimental tooth movement in rats

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1625 ◽  
Author(s):  
Martina Von Böhl ◽  
Yijin Ren ◽  
Anne M. Kuijpers-Jagtman ◽  
Piotr S. Fudalej ◽  
Jaap C. Maltha
Keyword(s):  
Dental Pulp ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Inflammatory Cells ◽  
Contralateral Side ◽  
Pulp Tissue ◽  
Adult Rats ◽  
Force Application ◽  
Age Related ◽  
Age Related Changes

It is generally accepted that the effect of orthodontic tooth movement on the dental pulp in adolescents is reversible and that it has no long-lasting effect on pulpal physiology. However, it is not clear yet if the same conclusion is also valid for adult subjects. Thus, in two groups of rats, aged 6 and 40 weeks respectively, 3 molars at one side of the maxilla were moved together in a mesial direction with a standardized orthodontic appliance delivering a force of 10 cN. The contralateral side served as a control. Parasagittal histological sections were prepared after tooth movement for 1, 2, 4, 8, and 12 weeks. The pulp tissue was characterized for the different groups, with special emphasis on cell density, inflammatory cells, vascularity, and odontoblasts. Dimensions of dentin and the pulpal horns was determined and related with the duration of orthodontic force application and age ware evaluated. We found that neither in young nor in adult rats, force application led to long-lasting or irreversible changes in pulpal tissues. Dimensional variables showed significant age-related changes. In conclusion, orthodontic tooth movement per se has no long-lasting or irreversible effect on pulpal tissues, neither in the young nor in the adult animals.

scholarly journals Molecular Markers of Dental Pulp Tissue during Orthodontic Tooth Movement: A Pilot Study

2012 ◽  
Vol 2012 ◽  
pp. 1-4
Author(s):  
Rohaya Megat Abdul Wahab ◽  
Shahrul Hisham Zainal Ariffin ◽  
Wong Woan Yeen ◽  
Nurul Atikah Ahmad ◽  
Sahidan Senafi
Keyword(s):  
Molecular Markers ◽  
Pilot Study ◽  
Differentially Expressed Genes ◽  
Dental Pulp ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Differentially Expressed ◽  
Pulp Tissue ◽  
Molecular Changes ◽  
Dental Pulp Tissue

Three specific orthodontic tooth movement genes, that is,FCRL1,HSPG2, andLAMB2were detected at upper first premolar (with appliance) dental pulp tissue by using GeneFishing technique as compared to lower first premolar (without appliance). These three differentially expressed genes have the potential as molecular markers during orthodontic tooth movement by looking at molecular changes of pulp tissue.

scholarly journals Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation:In VivoStudy

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Daniel Chavarria-Bolaños ◽  
Hector Flores-Reyes ◽  
Nelson Lombana-Sanchez ◽  
Bernardino Cerda-Cristerna ◽  
Amaury Pozos-Guillen
Keyword(s):  
Dental Pulp ◽  
Endogenous Opioids ◽  
Control Group ◽  
Pulp Tissue ◽  
Calcitonin Gene ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp ◽  
Increasing Trends ◽  
Inflammation Group ◽  
Different Levels

Purpose. This study quantified the expression of substance P (SP), calcitonin gene-related peptide (CGRP),β-endorphins (β-End), and methionine-enkephalin (Met-Enk) in human dental pulp following orthodontic intrusion.Methods. Eight patients were selected according to preestablished inclusion criteria. From each patient, two premolars (indicated for extraction due to orthodontic reasons) were randomly assigned to two different groups: the asymptomatic inflammation group (EXPg), which would undergo controlled intrusive force for seven days, and the control group (CTRg), which was used to determine the basal levels of each substance. Once extracted, dental pulp tissue was prepared to determine the expression levels of both neuropeptides and endogenous opioids by radioimmunoassay (RIA).Results. All samples from the CTRg exhibited basal levels of both neuropeptides and endogenous opioids. By day seven, all patients were asymptomatic, even when all orthodontic-intrusive devices were still active. In the EXPg, the SP and CGRP exhibited statistically significant different levels. Although none of the endogenous opioids showed statistically significant differences, they all expressed increasing trends in the EXPg.Conclusions. SP and CGRP were identified in dental pulp after seven days of controlled orthodontic intrusion movement, even in the absence of pain.

scholarly journals Upregulation of ETV2 Expression Promotes Endothelial Differentiation of Human Dental Pulp Stem Cells

2021 ◽  
Vol 30 ◽  
pp. 096368972097873
Author(s):  
Jing Li ◽  
Youming Zhu ◽  
Na Li ◽  
Tao Wu ◽  
Xianyu Zheng ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Tube Formation ◽  
Endothelial Differentiation ◽  
Cell Source ◽  
Lentiviral Infection ◽  
Mrna And Protein Expression ◽  
Human Dental Pulp

The lack of vasculogenesis often hampers the survivability and integration of newly engineered tissue grafts within the host. Autologous endothelial cells (ECs) are an ideal cell source for neovascularization, but they are limited by their scarcity, lack of proliferative capacity, and donor site morbidity upon isolation. The objective of this study was to determine whether differentiation of human dental pulp stem cells (DPSCs) into the endothelial lineage can be enhanced by recombinant ETV2 overexpression. DPSCs were extracted from fresh dental pulp tissues. ETV2 overexpression in DPSCs was achieved by lentiviral infection and cellular morphological changes were evaluated. The mRNA and protein expression levels of endothelial-specific markers were assessed through quantitative real-time polymerase chain reaction, western blot, immunofluorescence staining, and flow cytometry. The tube formation assay and Matrigel plug assay were also performed to evaluate the angiogenic potential of the ETV2-transduced cells in vitro and in vivo, respectively. Additionally, proteomic analysis was performed to analyze global changes in protein expression following ETV2 overexpression. After lentiviral infection, ETV2-overexpressing DPSCs showed endothelial-like morphology. Compared with control DPSCs, significantly higher mRNA and protein expression levels of endothelial-specific genes, including CD31, VE-Cadherin, VEGFR1, and VEGFR2, were detected in ETV2-overexpressing DPSCs. Moreover, ETV2 overexpression enhanced capillary-like tube formation on Matrigel in vitro, as well as neovascularization in vivo. In addition, comparative proteomic profiling showed that ETV2 overexpression upregulated the expression of vascular endothelial growth factor (VEGF) receptors, which was indicative of increased VEGF signaling. Taken together, our results indicate that ETV2 overexpression significantly enhanced the endothelial differentiation of DPSCs. Thus, this study shows that DPSCs can be a promising candidate cell source for tissue engineering applications.

Flow cytometric analysis of human dental pulp tissue

1991 ◽  
Vol 17 (2) ◽  
pp. 49-53 ◽  
Author(s):  
Chutima Mangkornkarn ◽  
James C. Steiner ◽  
Roger Bohman ◽  
Robert A. Lindemann
Keyword(s):  
Dental Pulp ◽  
Flow Cytometric Analysis ◽  
Pulp Tissue ◽  
Flow Cytometric ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp

Copper-zinc superoxide dismutase activity in normal and inflamed human dental pulp tissue

1991 ◽  
Vol 17 (7) ◽  
pp. 316-318 ◽  
Author(s):  
Walter L. Davis ◽  
Bennett H. Jacoby ◽  
Kathleen R. Craig ◽  
Gary Wagner ◽  
John W. Harrison
Keyword(s):  
Superoxide Dismutase ◽  
Dental Pulp ◽  
Superoxide Dismutase Activity ◽  
Pulp Tissue ◽  
Copper Zinc Superoxide Dismutase ◽  
Zinc Superoxide Dismutase ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp ◽  
Copper Zinc

Action of nitric oxide on healthy and inflamed human dental pulp tissue

Micron ◽  
2008 ◽  
Vol 39 (7) ◽  
pp. 797-801 ◽  
Author(s):  
Leopoldo Penteado Nucci da Silva ◽  
João Paulo Mardegan Issa ◽  
Elaine Aparecida Del Bel
Keyword(s):  
Nitric Oxide ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp

scholarly journals Effects of salbutamol, montelukast and prednisone on orthodontic tooth movement in rats

2021 ◽  
Vol 24 (2) ◽  
Author(s):  
Victor Chumpitaz-Cerrate ◽  
Lesly Chávez-Rimache ◽  
César Franco-Quino ◽  
Eliberto Ruiz-Ramirez ◽  
Elías Aguirre-Siancas ◽  
...  
Keyword(s):  
Clinical Evaluation ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Study Groups ◽  
Preclinical Study ◽  
Control Group ◽  
Histopathological Analysis ◽  
Significant Difference ◽  
Interincisal Distance

Objective: To evaluate the effect of salbutamol, montelukast, and prednisone on orthodontic tooth movement in rats. Material and Methods: In vivo experimental preclinical study. The sample consisted of 48 rats randomly distributed in four study groups. Group A was given saline solution; to group B, salbutamol 4 mg/Kg; to group C, montelukast 2.5 mg/Kg and to group D, prednisone 2.5 mg/Kg. All were fitted with orthodontic devices and the medications were administered intraperitoneally every 12 hours for 5 days. The clinical evaluation (variation in the interincisal distance) was performed at one, three, five, and seven days and the histopathological analysis (cell count) at five and seven days. Results: In the clinical evaluation of the variation in the interincisal distance, a significant difference was found in all the evaluations (p <0.05). It was found that the salbutamol group presented higher variation values in the interincisal distance on all the days evaluated. In the histopathological analysis at five and seven days, it was found that the osteoblast and osteocyte count was significantly higher in the salbutamol group compared to the other groups (p ><0.05). However, in the subgroup analysis, it was found that there was no significant difference in the osteoblast and osteocyte count between the prednisone, montelukast, and control group (p>0.05). Conclusion: The administration of salbutamol increased the magnitude of orthodontic tooth movement; nonetheless, the administration of montelukast and prednisone did not modify the magnitude of orthodontic tooth movement in rats.   Keywords Albuterol; Montelukast; Prednisone; Rats; Tooth movement.

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