Nasal Carriage of Community Acquired and Inducible Dormant Methicillin Resistant Staphylococcus aureus among Healthcare Workers of Mansoura University Children's Hospital

Abstract Objective: To determine the nasal carriage of staphylococcus aureus and methicillin-resistant staphylococcus aureus among healthcare workers in a tertiary care setting. Methods: The cross-sectional study was conducted at the Pakistan Institute of Medical Sciences, Islamabad, Pakistan, from April to July 2018, and comprised healthcare workers at the institution. Nasal swabs were collected and cultured on Mannitol salt agar. Mannitol fermenting colonies which were gram-positive cocci, catalase-positive and coagulase-positive were identified as staphylococcus aureus. Antibiotic susceptibility test was performed by modified Kirby-Bauer disc diffusion method. Methicillin resistance was detected using cefoxitin disc diffusion method. Data was analysed using SPSS 23. Results: Of the 210 nasal swabs, 52(24.76%) had a staphylococcus aureus growth, and, of them, 15(7.1%) were methicillin-resistant. No association could be established with either any single category of healthcare worker or an inter-department variation (p>0.05). Likewise, there was no association with age, gender, duration of service, smoking, co-morbidities, use of antibiotics in the preceding six months, treating a patient with methicillin-resistant staphylococcus aureus in the preceding six months and hospitalisation in the preceding year (p>0.05). Conclusion: The frequency of nasal carriage of methicillin resistant staphylococcus aureus amongst healthcare workers was regardless of the nature of their professional engagement. Key Words: Methicillin resistant staphylococcus aureus, Nasal carriage, Continuous...

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Continued Decline in Community-Acquired Invasive Methicillin-Resistant Staphylococcus aureus (MRSA) Infections at Texas Children's Hospital

Open Forum Infectious Diseases ◽

10.1093/ofid/ofw172.535 ◽

2016 ◽

Vol 3 (suppl_1) ◽

Author(s):

Kristina G. Hulten ◽

Edward Mason ◽

Linda B. Lamberth ◽

Sheldon L. Kaplan

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistant ◽

Children's Hospital ◽

Children’S Hospital

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Incidence of methicillin-resistant Staphylococcus aureus infection in a children’s hospital in the Washington metropolitan area of the United States, 2003 – 2010

Emerging Microbes & Infections ◽

10.1038/emi.2013.69 ◽

2013 ◽

Vol 2 (1) ◽

pp. 1-5 ◽

Cited By ~ 3

Author(s):

Xiaoyan Song ◽

Jonathan Cogen ◽

Nalini Singh

Keyword(s):

United States ◽

Staphylococcus Aureus ◽

Metropolitan Area ◽

Methicillin Resistant Staphylococcus Aureus ◽

The United States ◽

Methicillin Resistant ◽

Children's Hospital ◽

Aureus Infection ◽

Children’S Hospital

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PREVALENCE OF NASAL CARRIAGE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AMONG HEALTHCARE WORKERS IN THE DEPARTMENTS OF OTORINOLARYNGOLOGY AND DENTISTRY IN KYIV, UKRAINE

Wiadomości Lekarskie ◽

10.36740/wlek202012101 ◽

2020 ◽

Vol 73 (12) ◽

pp. 2563-2567

Author(s):

Aidyn G. Salmanov ◽

Taras P. Bondar ◽

Yaroslav V. Shkorbotun ◽

Evelina A. Chumak ◽

Volodymyr O. Shkorbotun ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Virulence Factor ◽

Healthcare Workers ◽

Methicillin Resistant Staphylococcus Aureus ◽

Nasal Carriage ◽

Diffusion Method ◽

Methicillin Resistant ◽

Significant Difference ◽

Mrsa Strains ◽

Encoding Genes

The aim: To obtain the first estimates of the current prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) in the departments of Otorinolaryngology and Dentistry and to determine of genes virulence factors (Panton Valentine Leukocidine (PVL) genes). Materials and methods: We performed a multicenter cross-sectional study. The susceptibility to antibiotics was determined by disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing. The virulence factor encoding genes, mecA, lukS-lukF, were detected by Polymerase Chain Reaction (PCR). Results: Incidence rate of S. aureus nasal carriage among HCWs was 36.2%, whereas MRSA carriage was 17%. Prevalence of MRSA carriage rate was 34.9% in Otorhinolaryngology departments and 9.7% in Dentistry. PCR testing confirmed that all MRSA strains were mecA gene-positive. The virulence factor encoding genes were detected in 82.3% of the S. aureus isolates from HCWs. Among S.aureus, the lukS-lukF genes were detected in over 59% of the strains. The lukS-lukF genes were detected in 55.5% of MRSA and in 58.9% of MSSA strains. LukS-lukF genes were most commonly co-present in MRSA strains. No significant difference was detected between the occurrences of lukS-lukF genes (P > 0.05). Conclusions: Personnell in otorhinolaryngology and dentistry departments have a high rate of nasal colonization of MRSA. This carrier state may be an important risk factor for transmission MRSA from physicians and nurses to patients and vice-versa. Screening for MRSA nasal carriage of HCWs is a key element in enabling infection control measures and early therapeutic decisions.

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