scholarly journals Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

2020 ◽  
Vol 51 (2) ◽  
pp. 215-223
Author(s):  
Reza Mousavi ◽  
Ebrahim Rahimi ◽  
Amir Shakerian
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Poultry Meat ◽  
Escherichia Coli O157 ◽  
Virulence Markers

scholarly journals Comparison between human and animal isolates of Shiga toxin-producing Escherichia coli O157 from Australia

2002 ◽  
Vol 128 (3) ◽  
pp. 357-362 ◽  
Author(s):  
N. FEGAN ◽  
P. DESMARCHELIER
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Human Infection ◽  
Pulsed Field Gel Electrophoresis ◽  
Toxin Gene ◽  
Escherichia Coli O157 ◽  
Animal Population ◽  
E Coli ◽  
Virulence Markers ◽  
Animal Isolates

There is very little human disease associated with enterohaemorrhagic Escherichia coli O157 in Australia even though these organisms are present in the animal population. A group of Australian isolates of E. coli O157:H7 and O157:H- from human and animal sources were tested for the presence of virulence markers and compared by XbaI DNA macrorestriction analysis using pulsed-field gel electrophoresis (PFGE). Each of 102 isolates tested contained the gene eae which encodes the E. coli attaching and effacing factor and all but one carried the enterohaemolysin gene, ehxA, found on the EHEC plasmid. The most common Shiga toxin gene carried was stx2c, either alone (16%) or in combination with stx1 (74%) or stx2 (3%). PFGE grouped the isolates based on H serotype and some clusters were source specific. Australian E. coli O157:H7 and H- isolates from human, animal and meat sources carry all the virulence markers associated with EHEC disease in humans therefore other factors must be responsible for the low rates of human infection in Australia.

Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece

2009 ◽  
Vol 26 (8) ◽  
pp. 865-871 ◽  
Author(s):  
Nikolaos Solomakos ◽  
Alexandros Govaris ◽  
Apostolos S. Angelidis ◽  
Spyros Pournaras ◽  
Angeliki Rothi Burriel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Escherichia Coli O157

Antibiotic resistance of Escherichia coli O157:H7 isolated from cattle and sheep

2010 ◽  
Vol 60 (3) ◽  
pp. 489-494 ◽  
Author(s):  
Muammer Goncuoglu ◽  
Fatma Seda Bilir Ormanci ◽  
Naim Deniz Ayaz ◽  
Irfan Erol
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Escherichia Coli O157 ◽  
Cattle And Sheep

scholarly journals Molecular Profiling of Escherichia coli O157:H7 and Non-O157 Strains Isolated from Humans and Cattle in Alberta, Canada

2014 ◽  
Vol 53 (3) ◽  
pp. 986-990 ◽  
Author(s):  
Linda Chui ◽  
Vincent Li ◽  
Patrick Fach ◽  
Sabine Delannoy ◽  
Katarzyna Malejczyk ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Virulence Genes ◽  
Molecular Profiling ◽  
Surveillance Program ◽  
Escherichia Coli O157 ◽  
Significant Determinant ◽  
Content Type ◽  
Virulence Markers ◽  
Almost All

Virulence markers in Shiga toxin-producingEscherichia coli(STEC) and their association with diseases remain largely unknown. This study determines the importance of 44 genetic markers for STEC (O157 and non-O157) from human clinical cases and their correlation to disease outcome. STEC isolated from a cattle surveillance program were also included. The virulence genes tested were present in almost all O157:H7 isolates but highly variable in non-O157 STEC isolates. Patient age was a significant determinant of clinical outcome.

scholarly journals Comparison of virulence markers and antibiotic resistance in enterotoxigenic Escherichia coli isolated ten years apart in Tehran

2010 ◽  
Vol 5 (04) ◽  
pp. 248-254 ◽  
Author(s):  
Nader Shahrokhi ◽  
Saeid Bouzari ◽  
Anis Jafari
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Dna Hybridization ◽  
Pathogenic Bacteria ◽  
Enterotoxigenic Escherichia Coli ◽  
Significant Shift ◽  
Bacterial Populations ◽  
Virulence Markers ◽  
Class 1 ◽  
Resistance Patterns

Introduction: Enterotoxigenic Escherichia coli (ETEC) causes diarrhoea by producing heat-labile (LT) or heat-stable (ST) enterotoxins after colonizing the small intestine by means of colonization factors (CFs). Although detection of the toxins is sufficient for verification of ETEC isolates, toxin-positive strains may be further analyzed for the presence of CFs. Antibiotics may shorten the duration of diarrhoea caused by ETEC, but the rapid emergence of resistant strains limits their usefulness. Methodology: ETEC isolates collected 10 years apart were compared for the prevalence of toxin types, CFs and antibiotic resistance. DNA/DNA hybridization with digoxigenin (DIG)-labeled probes was used for the detection of toxin types, and CF-typing was performed by DNA hybridization using DIG-labeled probes for cfaD and CS6 with slide agglutination. Disk diffusion was used to determine antibiotic resistance. The presence of class 1 integrons was detected by PCR. Results: ST-positive isolates were the most prevalent among the isolates from 1988, but a significant shift towards LT-gene carriage was observed in the 1998 group. CFA/I and CFA/IV were the most common CF types within both groups. The most prevalent resistance patterns among these isolates were ACSTSXT followed by ASTSXT and ASSXT. Conclusion: Our study of the two groups of isolates showed that the rate of LT and ST gene carriage, as well as antibiotic resistance markers, has changed in the ten years separating the two bacterial populations. These variations show the importance of monitoring pathogenic bacteria to obtain a near realistic picture of the circulating bacterial pathogens.

scholarly journals Prevalence and antibiotic resistance profile of Shiga-toxigenic Escherichia coli O157 (STEC) from retailed miscellaneous meat and fish types in Abuja, Nigeria

2021 ◽  
Vol 2 (2) ◽  
pp. 37-43
Author(s):  
Adaeze Joy Alu ◽  
Gabriel K. Omeiza ◽  
James A. Ameh ◽  
Enem S.I
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Enrichment Culture ◽  
Intestinal Bacteria ◽  
Latex Agglutination ◽  
Multi Drug Resistance ◽  
Escherichia Coli O157 ◽  
Resistance Profile ◽  
E Coli ◽  
Antibiotic Resistance Profile

Most Escherichia coli strains are harmless intestinal bacteria of animals, but some are implicated in food infection/poisoning especially Shiga toxin (or Vero toxin) producing E. coli (STEC) due to consumption of meat. This study was conducted to determine the prevalence and antibiotic resistance profile of Shigatoxigenic Escherichia coli O157 (STEC) from retailed miscellaneous fish and meat types in Abuja, Federal Capital Territory, Nigeria. A total of 256 meat and fish consisting of cow muscles, intestines, rumen-sacs, livers and tails, cat-fish, frozen fish (mackerel and herrings) were examined. Escherichia coli were isolated by enrichment culture cefixime-tellurite sorbitol MacConkey agar (CT-SMAC), morphological, biochemical, serotype latex agglutination and disk diffusion methods. Of the 256 samples, 138 (53.9%) were contaminated with E. coli and 28 (21.7%) E. coli strains were positive for Shigatoxigenic Escherichia coli O157 (STEC). Meat muscles had the highest prevalence of STEC (7.41%) among meat samples, followed by rumen-sacs (6.0%), intestines (5.77%), tails (4.0%), and the prevalence of STEC in Fish includes Cat-fish intestine (26.7%), skin (21.4%), Mackerel intestine (26.7%), skin (14.3%), and Herrings skin (15.4%), gill (7.1%). All the STEC assessed indicated multi-drug resistance, with the isolates showing 100% resistant to ampicilin, and erythromycin, nitrofurantoin (95.7%), amoxicilin clavulanic acid (84.3%), sulphamethaxazole/trimethoprim (75%), streptomycin (75%), tetracycline (66.17%), and gentamycin (53.6%). The isolates were susceptible to ciprofloxacin (66.7%), Cefoxitin (66.7%), amikacin (39.3%), and chloramphenicol (35.7%). The implication of STEC in this study suggests that contaminated meat types are sold to consumers and can result to serious foodborne hazards. Prescription of ciprofloxacin and cefoxicilin are recommended against this organism. Application of good hygienic procedures in meat and fish handling processes and proper boiling before consumption can mitigate the risk of infection due to resistance STEC strains.

Characterization of Escherichia coli and Salmonella from Victoria, Australia, Dairy Farm Environments

2017 ◽  
Vol 80 (12) ◽  
pp. 2078-2082 ◽  
Author(s):  
Catherine M. McAuley ◽  
Kate E. McMillan ◽  
Sean C. Moore ◽  
Narelle Fegan ◽  
Edward M. Fox
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Dairy Farm ◽  
Farm Animals ◽  
E Coli ◽  
Virulence Markers ◽  
Salmonella Serotypes ◽  
Serotype Identification ◽  
Salmonella Serotype ◽  
Genetic Profiles

ABSTRACT Safe dairy food production starts at the farm level, with the presence of pathogens on farms potentially impacting the downstream food supply. Studies often commence with looking for pathogens in fecal material of farm animals, predominantly cows; however, pathogens may arise from other on-farm sources. In Australia, few studies have looked at the broader farm environment, particularly in relation to Escherichia coli and Salmonella. The present study characterized the genetic similarity of these pathogens from bovine, ovine, and caprine dairy farm environments and related this to the stx1, stx2, eae, or ehx virulence markers in E. coli and antibiotic resistance in Salmonella. E. coli isolates with indistinguishable genetic profiles and at least one of the virulence factors were found in multiple samples on the farms, although profiles were unique to each farm. E. coli O26 with stx1 from one bovine farm had a different fingerprint type than all of the other E. coli O26 isolates, which lacked the Shiga toxin genes. They were from a separate bovine farm and were themselves closely related. No antibiotic resistance was detected among Salmonella isolates to the 17 antibiotics tested. Three Salmonella serotypes were identified: Orion, Infantis, and Zanzibar. The published PCR serotyping method used misidentified Salmonella Zanzibar as Salmonella Javiana, which was revealed after conventional antisera-based serotyping; this illustrates the need for caution when using PCR techniques for Salmonella serotype identification. Of the three serotypes, Salmonella Orion was most prevalent and was potentially resident on the farm. This article describes the previously unreported genetic diversity of potentially pathogenic E. coli and Salmonella serotypes from the farm environments of three dairy animal species in Victoria, Australia.

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