scholarly journals Evaluation of the effectiveness of pharmacological correction of experimental osteoporosis by glucosamine sulfate, strontium chloride, and their combination

2020 ◽  
pp. 50-56
Author(s):  
Aleksandr V. Anikanov ◽  
Elena B. Artyushkova ◽  
Aleksandr V. Faitelson ◽  
Densingh Samuel Raj Rajkumar
Keyword(s):  
Bone Density ◽  
Drug Prevention ◽  
Glucosamine Sulfate ◽  
Laboratory Animals ◽  
Strontium Chloride ◽  
Average Width ◽  
Doppler Flowmetry ◽  
Experimental Osteoporosis ◽  
Prevention Of Osteoporosis ◽  
Bone Trabeculae

Objective. To study the osteoprotective activity of glucosamine sulfate, strontium chloride, and their combinations on a model of experimental osteoporosis in laboratory animals (OVX model) in comparison with the Strometta preparation and control (OVX without correction). Materials and methods. The material for the study was female Wistar rats weighing 250 ± 30 g. To simulate osteoporosis, bilateral ovariectomy was performed. Microcirculation was evaluated by Doppler flowmetry. Digital X-ray densitometry was used to determine bone density. Using histological morphometry, the transverse size of the trabeculae of the spongy bone tissue was studied. Results. In such parameters as bone density, microcirculation, and the average width of bone trabeculae, significant differences were established compared with the control (OVX without correction) for groups with correction of glucosamine sulfate, strontium chloride, and their combination, and thus their osteoprotective effect was confirmed. Conclusion. In the future, glucosamine sulfate, strontium chloride, and their combination can be used for drug prevention of osteoporosis in the postmenopausal period. However, for the introduction of strontium chloride and its combination with glucosamine sulfate into clinical practice, it is necessary to conduct complete preclinical and clinical trials.

scholarly journals AT1 and AT2 Receptor Knockout Changed Osteonectin and Bone Density in Mice in Periodontal Inflammation Experimental Model

2021 ◽  
Vol 22 (10) ◽  
pp. 5217
Author(s):  
Maria Laura de Souza Lima ◽  
Caroline Addison Carvalho Xavier de Medeiros ◽  
Gerlane Coelho Bernardo Guerra ◽  
Robson Santos ◽  
Michael Bader ◽  
...  
Keyword(s):  
Bone Density ◽  
Bone Loss ◽  
Experimental Model ◽  
At2 Receptor ◽  
Micro Ct ◽  
Rt Pcr ◽  
Osteoblast Cells ◽  
Periodontal Inflammation ◽  
Bone Trabeculae

Background: The aim of this study was to evaluate the role of AT1 and AT2 receptors in a periodontal inflammation experimental model. Methods: Periodontal inflammation was induced by LPS/Porphyromonas gingivalis. Maxillae, femur, and vertebra were scanned using Micro-CT. Maxillae were analyzed histopathologically, immunohistochemically, and by RT-PCR. Results: The vertebra showed decreased BMD in AT1 H compared with WT H (p < 0.05). The femur showed increased Tb.Sp for AT1 H and AT2 H, p < 0.01 and p < 0.05, respectively. The Tb.N was decreased in the vertebra (WT H-AT1 H: p < 0.05; WT H-AT2 H: p < 0.05) and in the femur (WT H-AT1 H: p < 0.01; WT H-AT2 H: p < 0.05). AT1 PD increased linear bone loss (p < 0.05) and decreased osteoblast cells (p < 0.05). RANKL immunostaining was intense for AT1 PD and WT PD (p < 0.001). OPG was intense in the WT H, WT PD, and AT2 PD when compared to AT1 PD (p < 0.001). AT1 PD showed weak immunostaining for osteocalcin compared with WT H, WT PD, and AT2 PD (p < 0.001). AT1 H showed significantly stronger immunostaining for osteonectin in fibroblasts compared to AT2 H (p < 0.01). Conclusion: AT1 receptor knockout changed bone density, the quality and number of bone trabeculae, decreased the number of osteoblast cells, and increased osteonectin in fibroblasts.

scholarly journals General Influence of Biphasic Calcium Phosphate on Osteoporotic Bone Density

2019 ◽  
Vol 73 (2) ◽  
pp. 185-188
Author(s):  
Vladislavs Ananjevs ◽  
Aleksandra Ananjeva ◽  
Jānis Vētra ◽  
Andrejs Skaģers ◽  
Ilze Salma ◽  
...  
Keyword(s):  
Bone Density ◽  
Mass Density ◽  
Cone Beam ◽  
Bone Mass Density ◽  
Osteoporotic Bone ◽  
Left Femur ◽  
Experimental Osteoporosis ◽  
The Right ◽  
General Influence

Abstract Bone density of the femur body of rabbit was determined in vivo. Experimental osteoporosis was induced by ovariectomy and subsequent injections of methylprednisolone. In the greater trochanter region of right femur, defects were created and filled with granules of hydroxyapatite and tricalcium phosphate (HAP/TCP 70/30) or HAP/TCP 70/30 together with 5% strontium. After three months, the animals were euthanized. The bone mass density of the right and left body of femur was measured by cone beam computed tomography (CT) scan. The results of the study showed that the right femur of the rabbit, where biomaterials had been implanted, and the left femur, where no biomaterial implantation occurred, became denser after filling the defect with HAP/TCP 70/30 ceramic granules or 5% Sr modified HAP/TCP ceramic granules. There was no difference between operated and non-operated legs and HAP/TCP and HAP/TCP with 5% strontium groups.

Comparative Assessment of Endothelium-Associated Correction of Experimental Osteoporosis with Resveratrol and Etoksidol

2012 ◽  
Vol 19 (1) ◽  
pp. 8-11
Author(s):  
Aleksandr Vladimirovich Faytel'son ◽  
G M Dubrovin ◽  
O S Gudyrev ◽  
M V Pokrovskiy ◽  
A V Ivanov ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Bone Tissue ◽  
Bone Remodeling ◽  
Wistar Rats ◽  
Comparative Assessment ◽  
Experimental Osteoporosis ◽  
Female Wistar Rats ◽  
Bone Trabeculae

Osteoprotective action of resveratrol and etoksidol was studied on the model of experimental osteoporosis (140 female Wistar rats). It was detected that endothelial dysfunction of bone tissue microcirculation bed that led to osteoporosis development. In contrast to etoksidol, resveratrol prevented the reduction of bone tissue microcirculation level and the aggravation of bone remodeling processes in developing osteoporosis that was manifested by delayed bone trabeculae thinning and averting of microfractures in them.

Laser-based non-invasive spectrophotometry – An overview of possible medical applications

2013 ◽  
Vol 2 (3) ◽  
Author(s):  
Dmitry Rogatkin ◽  
Vyacheslav Shumskiy ◽  
Sergey Tereshenko ◽  
Pavel Polyakov
Keyword(s):  
Diagnostic System ◽  
Medical Application ◽  
Scientific Data ◽  
Laser Fluorescence ◽  
Laboratory Animals ◽  
Differential Diagnostics ◽  
Doppler Flowmetry ◽  
Non Invasive ◽  
Blood Microcirculation ◽  
Combination Of Methods

AbstractThe problem of the medical application of non-invasive (The specially devised multifunctional laser diagnostic system “LAKK-M” was used as a diagnostic instrument in the majority of studies in MONIKI, allowing a combination of methods, such as non-invasive medical spectrophotometry in the form of laser fluorescence spectroscopy, laser Doppler flowmetry, tissue reflectance oximetry, etc. The system was used in both the experiments on laboratory animals, and for treatment of patients in different clinics of the institute.Within the last decade, extensive scientific data has been obtained which has opened up the possibility of using non-invasive medical spectrophotometryMethods of complex non-invasive medical spectrophotometry appear to be an efficient tool in practical medicine for differential diagnostics of a number of diseases and pathologies, as well as for monitoring and prediction of the treatment outcome. At the same time, they also create an important perspective for gaining novel and fundamental knowledge about the blood microcirculation system which was not available earlier due to the absence of the diagnostic technology allowing information to be obtained

scholarly journals Study of Endothelio- and Osteoprotective Effects of Combination of Rosuvastatin with L-Norvaline in Experiment

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
M. S. Sobolev ◽  
A. V. Faitelson ◽  
O. S. Gudyrev ◽  
D. S. R. Rajkumar ◽  
G. M. Dubrovin ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Bone Tissue ◽  
Wistar Rats ◽  
Microcirculatory Bed ◽  
Experimental Osteoporosis ◽  
Bone Trabeculae

The experiment was carried out on 120 female white Wistar rats, to study the endothelio- and osteoprotective action of the combination of rosuvastatin with L-norvaline in the model of experimental osteoporosis. It was found that, after ovariectomy in rats, endothelial dysfunction of the vessels of the microcirculatory bed of bone tissue develops, leading to the appearance of osteoporosis, but the combination of the studied drugs prevents the decrease in the level of microcirculation in the bone tissue, thereby preventing the thinning of bone trabeculae and preventing the occurrence of microfractures in them.

scholarly journals REPARATION OF BONE TISSUE IN THE AREA OF OPEN FRACTURE CONSOLIDATION IN TUBULAR BONE IN LABORATORY ANIMALS. AN EXPERIMENTAL STUDY

2020 ◽  
Vol 24 (2) ◽  
pp. 96-100
Author(s):  
Ivan I. Gordienko ◽  
N. A. Tsap ◽  
S. A. Borisov ◽  
I. E. Valamina
Keyword(s):  
Connective Tissue ◽  
Bone Tissue ◽  
Femoral Fracture ◽  
Guinea Pigs ◽  
Open Fracture ◽  
Laboratory Animals ◽  
Experimental Animals ◽  
Group I ◽  
Bone Trabeculae ◽  
Inflammatory Changes

Objective. Antibiotic prophylactics in surgery is a standard of medical care for many surgical interventions. It is “a very short course”: starts immediately before surgery and lasts no more than 24 hours after it. There were no any information found after a broad search on pathomorphology of consolidation zone in open fracture and morphometry of bone callus structures under various modalities of perioperative antibiotic prophylactics. Purpose. To find out reparation degree and inflammatory changes in bone tissue in the area of consolidation in open femoral fracture in experimental animals under various modalities of perioperative antibiotic prophylactics. Material and methods. 60 outbred male guinea pigs were taken into the experiment. The experimental animals were divided into 3 groups, 20 individuals in each. Animals from Group I, so-called “AB-1”, were given antibiotics 30 minutes before surgery and then for 8 hours after it during one day. Animals from Group II, so-called “AB-3” were administered antibacterial drug for 3 days of postoperative period. Guinea pigs from Group III (Controls) had no antibiotic therapy. The modeling of femur open fracture was performed according to the developed methodology for modeling and treatment of open fractures of tubular bones in experiment. Results. When comparing reparative processes in AB-1 and AB-3 groups, similar regeneration processes in femoral fracture were noted. They were accompanied by: formation of endostal and periosteal callus with abundantly vascularized connective tissue; formation of significant amount of newly formed bone trabeculae; insignificant presence of leukocytes in the lumen of bone marrow canal, what may be explained by peculiarities of postoperative management of laboratory animals. In the Control group, growth of newly formed bone trabeculae was less intensive ( less by 16.3%); there also were 2-fold increase in connective tissue volume and actively proliferating zones of cartilage tissue. Conclusion. Thus, findings of morphological examination indicate that in the experimental group with short 24-hour perioperative antibiotic prophylaxis, bone tissue consolidation had minimal inflammatory changes and better formation of new bone trabeculae.

The Role of Vitamins in the Prevention of Osteoporosis – a Brief Status Report

1999 ◽  
Vol 69 (3) ◽  
pp. 194-197 ◽  
Author(s):  
Weber
Keyword(s):  
Vitamin D ◽  
Bone Density ◽  
Vitamin C ◽  
Vitamin K ◽  
Bone Health ◽  
Intervention Studies ◽  
Epidemiological Studies ◽  
Bone Status ◽  
Prevention Of Osteoporosis

This papers summarizes the main role vitamins are believed to play in the prevention of osteoporosis, a common disease which is anticipated to rapidly increase because of the aging of the population. Vitamin D, the classical vitamin related to bone health, improves bone strength mainly by increasing intestinal calcium absorption and reabsorption of calcium by the kidney. Several intervention studies demonstrated in humans that vitamin D can improve bone status as measured by bone density. Vitamin C is considered an essential cofactor of collagen formation. Epidemiological studies report a positive association between vitamin C intake and bone density. Intervention studies on the effect of vitamin C on bone status are missing. Vitamin B6 could function as a cofactor to build up cross-links. In humans, however, there is little evidence to support this. Vitamin K is required for the biological activity of several coagulation factors; the classical function of vitamin K. Recent research also points to a role of vitamin K in bone metabolism. Vitamin K mediates the ¨-carboxylation of glutamyl residues on several bone proteins, notably osteocalcin. Epidemiological studies and results from first intervention trials are consistently suggesting that vitamin K may improve bone health.

scholarly journals Comparative Evaluation of Enalapril and Losartan in Pharmacological Correction of Experimental Osteoporosis and Fractures of Its Background

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
D. S. R. Rajkumar ◽  
A. V. Faitelson ◽  
O. S. Gudyrev ◽  
G. M. Dubrovin ◽  
M. V. Pokrovski ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Comparative Evaluation ◽  
Osteoporotic Fractures ◽  
Experimental Models ◽  
Female Rats ◽  
Experimental Osteoporosis ◽  
Pharmacological Correction ◽  
Bone Trabeculae

In the experiment on the white Wistar female rats (222 animals), the osteoprotective effect of enalapril and losartan was studied on experimental models of osteoporosis and osteoporotic fractures. It was revealed that in rats after ovariectomy, the endothelial dysfunction of microcirculation vessels of osteal tissue develops, resulting in occurrence of osteoporosis and delay of consolidation of experimental fractures. Enalapril and losartan prevented the reduction of microcirculation in bone, which was reflected in slowing the thinning of bone trabeculae and in preventing the occurrence of these microfractures, as well as increasing quality of experimental fractures healing.

Experts Advise on Bone Density, Fracture Risk

2005 ◽  
Vol 38 (10) ◽  
pp. 36-37
Author(s):  
KERRI WACHTER
Keyword(s):  
Bone Density ◽  
Fracture Risk
Sign in / Sign up

Export Citation Format

Share Document