scholarly journals Evaluation of four cell lines for assay of infectious adenoviruses in water samples

2009 ◽  
Vol 7 (4) ◽  
pp. 650-656 ◽  
Author(s):  
Sunny C. Jiang ◽  
Jijun Han ◽  
Jian-Wen He ◽  
Weiping Chu
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Aquatic Environment ◽  
Plaque Assay ◽  
Environmental Water ◽  
Recreational Waters ◽  
293A Cell ◽  
Human Lung Carcinoma ◽  
Serotype 5 ◽  
Human Lung Carcinoma Cell

Human viral contamination in drinking and recreational waters poses health risks. The application of PCR-based molecular technology has advanced our knowledge of the occurrence and prevalence of human viruses in water; however, it has provided no information on viral viability and infectivity. Four human cell lines were compared for their sensitivity to different serotypes of human adenoviruses using the TCID50 test. The sensitivity of each cell line varied with different serotypes of adenovirus. Human embryonic kidney cell line 293A and human lung carcinoma cell line A549 were the most sensitive, especially to enteric adenovirus 40 and 41. Plaque assay of primary sewage samples showed 293A can detect viral plaques in 7 of 13 primary sewage samples tested. Adenoviruses were also isolated using 293A from environmental water concentrates. Cloning and sequencing of environmental adenoviral isolates indentified them to be aligned with adenoviruses serotype 40 and serotype 5. The result of this study suggests that plaque assay with 293A cell line is suitable for detection of adenovirus in the aquatic environment. Combining this cell culture with molecular methods for viral assay in the aquatic environment will provide critical information for risk assessment.

scholarly journals The effectiveness of antiviral agents with broad-spectrum activity against chikungunya virus varies between host cell lines

2018 ◽  
Vol 26 ◽  
pp. 204020661880758 ◽  
Author(s):  
Evelyn J Franco ◽  
Jaime L Rodriquez ◽  
Justin J Pomeroy ◽  
Kaley C Hanrahan ◽  
Ashley N Brown
Keyword(s):  
Antiviral Activity ◽  
Host Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Broad Spectrum ◽  
Chikungunya Virus ◽  
Plaque Assay ◽  
A549 Cells ◽  
Western Hemisphere ◽  
Viral Burden

Chikungunya virus (CHIKV) is a mosquito-borne virus that has recently emerged in the Western Hemisphere. Approved antiviral therapies or vaccines for the treatment or prevention of CHIKV infections are not available. This study aims to evaluate the antiviral activity of commercially available broad-spectrum antivirals against CHIKV. Due to host cell-specific variability in uptake and intracellular processing of drug, we evaluated the antiviral effects of each agent in three cell lines. Antiviral activities of ribavirin (RBV), interferon-alfa (IFN-α) and favipiravir (FAV) were assessed in CHIKV-infected Vero, HUH-7, and A549 cells. CHIKV-infected cells were treated with increasing concentrations of each agent for three days and viral burden was quantified by plaque assay on Vero cells. Cytotoxic effects of RBV, FAV and IFN-α were also evaluated. Antiviral activity differed depending on the cell line used for evaluation. RBV had the greatest antiviral effect in HUH-7 cells (EC50 = 2.575 µg/mL); IFN-α was most effective in A549 cells (EC50 = 4.235 IU/mL); and FAV in HUH-7 cells (EC50 = 20.00 μg/mL). The results of our study show FAV and IFN-α are the most promising candidates, as their use led to substantial reductions in viral burden at clinically achievable concentrations in two human-derived cell lines. FAV is an especially attractive candidate for further investigation due to its oral bioavailability. These findings also highlight the importance of cell line selection for preclinical drug trials.

Decreased helenalin-induced cytotoxicity by flavonoids from Arnica as studied in a human lung carcinoma cell line

Phytomedicine ◽  
1995 ◽  
Vol 2 (2) ◽  
pp. 127-132 ◽  
Author(s):  
H.J. Woerdenbag ◽  
I. Merfort ◽  
T.J. Schmidt ◽  
C.M. Passreiter ◽  
G. Willuhn ◽  
...  
Keyword(s):  
Cell Line ◽  
Lung Carcinoma ◽  
Human Lung ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Lung Carcinoma Cell ◽  
Lung Carcinoma Cell Line ◽  
Human Lung Carcinoma ◽  
Human Lung Carcinoma Cell

scholarly journals Effect of Host Cells on Low- and Medium-Pressure UV Inactivation of Adenoviruses

2010 ◽  
Vol 76 (21) ◽  
pp. 7068-7075 ◽  
Author(s):  
Huiling Guo ◽  
Xiaona Chu ◽  
Jiangyong Hu
Keyword(s):  
Host Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Host Cells ◽  
Uv Disinfection ◽  
Human Embryonic Kidney ◽  
Experimental Conditions ◽  
Medium Pressure ◽  
Serotype 5 ◽  
Uv Dose

ABSTRACT UV disinfection is highly effective against most pathogens, with the exception of the adenoviruses (AD). To date, many studies have focused on low-pressure (LP) UV inactivation of AD, but little is known about the effect of medium-pressure (MP) UV inactivation of AD. Despite numerous studies of LP UV inactivation of AD, extreme variabilities in the LP UV dose requirements of AD had been observed because of differing experimental conditions used, such as the types of cell lines used for AD enumeration. This study therefore investigates the effect of three different host cell lines (PLC/PRF/5, human embryonic kidney 293 [HEK293], and XP17BE) on the LP and MP UV dose requirements of AD serotype 5 (AD5), AD40, and AD41 under similar experimental settings. Results showed that for 4-log inactivation of AD, LP UV and MP UV doses needed to be in the ranges of 123 to 182 mJ/cm2 and 65 to 90 mJ/cm2, respectively, when HEK293 and PLC/PRF/5 cells were used for enumeration. The UV doses required for MP UV inactivation of AD were significantly lower than those required for LP UV inactivation (P value < 0.05). When different cell lines were used for enumeration, UV dose requirements for AD differed. AD were portrayed to be most susceptible to UV (LP UV doses of <57 mJ/cm2 and MP UV doses of <42 mJ/cm2 for 4-log AD inactivation) when the XP17BE cells were used as the host cell. The use of different cell lines for AD enumeration affected LP UV dose results more significantly than MP UV dose results (P value < 0.05). Cell line variability factors for LP UV disinfection (CLLP) and MP UV disinfection (CLMP) for AD5, AD40, and AD41 enumerated with HEK293, PLC/PRF/5, and XP17BE cells were in the ranges of 1.0 to 3.2 and 1.0 to 2.5, respectively.

Apoptosis and cell cycle disturbances induced by coumarin and 7-hydroxycoumarin on human lung carcinoma cell lines

Lung Cancer ◽  
2004 ◽  
Vol 43 (3) ◽  
pp. 275-283 ◽  
Author(s):  
Jose Sullivan Lopez-Gonzalez ◽  
Heriberto Prado-Garcia ◽  
Dolores Aguilar-Cazares ◽  
Juan A Molina-Guarneros ◽  
Jorge Morales-Fuentes ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Lung Carcinoma ◽  
Human Lung ◽  
Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Lung Carcinoma Cell ◽  
Human Lung Carcinoma ◽  
Human Lung Carcinoma Cell
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