scholarly journals Inactivation of Escherichia coli in water by pulsed dielectric barrier discharge in coaxial reactor

2012 ◽  
Vol 10 (3) ◽  
pp. 371-379 ◽  
Author(s):  
A. N. Hernández-Arias ◽  
B. G. Rodríguez-Méndez ◽  
R. López-Callejas ◽  
D. Alcántara-Díaz ◽  
R. Valencia-Alvarado ◽  
...  

An experimental study of ATCC (American Type Culture Collection) 8739 Escherichia coli bacteria inactivation in water by means of pulsed dielectric barrier discharge (PDBD) atmospheric pressure plasmas is presented. Plasma is generated by an adjustable power source capable of supplying high voltage 25 kV pulses, ∼30 μs long and at a 500 Hz frequency. The process was conducted in a ∼152 cm3 cylindrical stainless steel coaxial reactor, endowed with a straight central electrode and a gas inlet. The bacterial concentration in water was varied from 103 up to 108E. coli cells per millilitre. The inactivation was achieved without gas flow in the order of 82% at 108 colony-forming units per millilitre (CFU mL–1) concentrations in 600 s. In addition, oxygen was added to the gas supply in order to increase the ozone content in the process, raising the inactivation percentage to the order of 90% in the same treatment time. In order to reach a higher efficiency however, oxygen injection modulation is applied, leading to inactivation percentages above 99.99%. These results are similarly valid for lower bacterial concentrations.

2019 ◽  
Vol 86 (1) ◽  
Author(s):  
Hao Wang ◽  
Liyang Zhang ◽  
Haiyun Luo ◽  
Xinxin Wang ◽  
Jinfeng Tie ◽  
...  

ABSTRACT With increasing attention toward novel sterilization methods, plasma sterilization has gained more and more interest. However, the underlying mechanisms are still unknown. In this paper, we investigated the inactivation of Escherichia coli using dielectric-barrier discharge (DBD) plasma in saline water. There were three processes shown in the survival curve, namely, during the preparation period, the reaction period, and the saturation period. Observations under a transmission electron microscope (TEM) and detection by Fourier transform infrared spectroscopy (FT-IR) supplied adequate details regarding these processes. Based on these results, we infer that during the preparation period, the main process is the accumulation of chemical substances. During the reaction period, adequate amounts of chemicals decompose and denature cell membranes and macromolecules to kill bacteria in large quantities. During the saturation period, the killing effect decreases because of the protection by clustered cells and the saturation of pH. This study of sterilizing processes systematically reveals the mechanisms of plasma sterilization. IMPORTANCE Compared with traditional methods, plasma sterilization has advantages of high efficiency, easy operation, and environmental protection. This may be more suitable for air and sewage sterilization in specific spaces, such as hospitals, laboratories, and pharmaceutical factories. However, the mechanisms of sterilization are still relatively unknown, especially for bactericidal activities. Knowledge of sterilization processes provides guidance for practical applications. For example, the bactericidal action mainly occurs during the reaction period, and the treatment time can be set based on the reaction period, which could save a lot of energy. The results of this study will help to improve the efficiency of plasma sterilization devices.


2013 ◽  
Vol 41 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Benjamín Gonzalo Rodriguez-Mendez ◽  
Alma Neli Hernandez-Arias ◽  
Régulo Lopez-Callejas ◽  
Raúl Valencia-Alvarado ◽  
Antonio Mercado-Cabrera ◽  
...  

2019 ◽  
Vol 47 (1) ◽  
pp. 395-402 ◽  
Author(s):  
Pietro Ranieri ◽  
Gerard McGovern ◽  
Henry Tse ◽  
Alexander Fulmer ◽  
Mykola Kovalenko ◽  
...  

2019 ◽  
Vol 21 (5) ◽  
pp. 055505 ◽  
Author(s):  
Songru XIE ◽  
Yong HE ◽  
Dingkun YUAN ◽  
Zhihua WANG ◽  
Sunel KUMAR ◽  
...  

2010 ◽  
Vol 42 ◽  
pp. 228-231 ◽  
Author(s):  
Yan Zhang ◽  
Yin Ding Lv

In this paper, polypropylene (PP) melt blown non-woven fabric is treated by atmospheric pressure N2 or N2/CO2 dielectric barrier discharge (DBD) plasma. The variation of the surface hydrophilicity of PP sample is experimentally investigated by surface water contact angle, Fourier transform infrared reflectance spectroscopy (FTIR-ATR). The results show that the hydrophilicity of PP sample is considerably improved as long as the very short plasma treatment time (several seconds). However, the treatment effect of atmospheric N2/CO2 plasma is worse than that of atmospheric N2 plasma.


Author(s):  
Koichi Takaki ◽  
Kota Nawa ◽  
Seiji Mukaigawa ◽  
Tamiya Fujiwara ◽  
Takeshi Aizawa

2019 ◽  
Vol 78 ◽  
pp. 02020
Author(s):  
Fada Feng ◽  
Xianjun He ◽  
Yingying Liang ◽  
Fengzhu Yang ◽  
Fushan Wang ◽  
...  

Microorganisms are widely distributed in the living environment of human, animals and plants. Some of the microorganisms are harmful for their pathogenic effects. Non-thermal plasma technology, especially, the atmospheric pressure plasma jet, is considered to be one of the promising technologies for sterilization. This work proposes a double-dielectric barrier discharge reactor for Ar plasma jet generation. Charteristics of discharge and temperature thermogram of plasma jet are investigated by the means of U-P curve and infrared image, respectively. Performance of the plasma jet is evaluated by surface and water sterilization. The results show that, Ar plasma jet is generated stable with double-dielectric barrier discharge. The length of plasma jet increases as the applied voltage, frequency or gas flow increased, but the plasma jet generation can be restricted in high frequency or gas flow. For E. coli in the water and surface, high sterilization efficiency is observed for a short time treatment by Ar plasma jet.


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