A Technique to Determine Unsteady-State Inhibition Kinetics in the Activated Sludge Process

1989 ◽  
Vol 21 (6-7) ◽  
pp. 593-602 ◽  
Author(s):  
Andrew T. Watkin ◽  
W. Wesley Eckenfelder
Keyword(s):  
Activated Sludge ◽  
Kinetic Parameters ◽  
Curve Fitting ◽  
Glucose Utilization ◽  
Batch Reactor ◽  
Synthetic Data ◽  
Utilization Rate ◽  
Fitting Algorithm ◽  
Test Reactor ◽  
Two Parameter

A technique for rapidly determining Monod and inhibition kinetic parameters in activated sludge is evaluated. The method studied is known as the fed-batch reactor technique and requires approximately three hours to complete. The technique allows for a gradual build-up of substrate in the test reactor by introducing the substrate at a feed rate greater than the maximum substrate utilization rate. Both inhibitory and non-inhibitory substrate responses are modeled using a nonlinear numerical curve-fitting technique. The responses of both glucose and 2,4-dichlorophenol (DCP) are studied using activated sludges with various acclimation histories. Statistically different inhibition constants, KI, for DCP inhibition of glucose utilization were found for the various sludges studied. The curve-fitting algorithm was verified in its ability to accurately retrieve two kinetic parameters from synthetic data generated by superimposing normally distributed random error onto the two parameter numerical solution generated by the algorithm.

Development of a respirometric assay to measure the transient load response of activated sludge to individual organic chemicals

1996 ◽  
Vol 33 (6) ◽  
pp. 49-55
Author(s):  
Barth F. Smets ◽  
Steven M. Fehniger ◽  
C. P. Leslie Grady
Keyword(s):  
Dissolved Oxygen ◽  
Activated Sludge ◽  
Kinetic Parameters ◽  
Transient Response ◽  
Batch Reactor ◽  
Oxygen Demand ◽  
Elevated Concentration ◽  
Fed Batch ◽  
Load Response ◽  
Oxygen Concentration Profile

This study described a new respirometric technique to monitor the transient response of activated sludge systems to individual synthetic organic compounds (SCOs). The assay used a fed-batch reactor system which contained mixed liquor from the parent reactor and received a biogenic feed and the target SOC at the same F/M ratio as was applied to the parent reactor. The fed-batch reactor was oxygenated using a hollow-fiber reaeration module to meet the steady-state oxygen demand. The reaeration module was characterized by means of KL a studies. The assay monitored the dissolved oxygen concentration profile after application of a shock load achieved by switching to a fed-batch feed with the target SOC at an elevated concentration. Mathematical descriptions of the transient profiles were derived and the experimentally observed dissolved oxygen profiles were subsequently compared to profiles predicted using extant kinetic parameters for SOC removal measured using an earlier developed assay. The use of the assay were complicated by the difficulty in maintaining a reaeration capacity at constant and low capacity. As a result, the adequacy of the measured extant kinetic parameters as predictors of the transient response could not be confirmed.

Biodegradation of o-nitrophenol by aerobic granules with glucose as co-substrate

2012 ◽  
Vol 65 (12) ◽  
pp. 2132-2139 ◽  
Author(s):  
Farrukh Basheer ◽  
M. H. Isa ◽  
I. H. Farooqi
Keyword(s):  
Activated Sludge ◽  
Kinetic Parameters ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Aerobic Granules ◽  
Haldane Model

Aerobic granules to treat wastewater containing o-nitrophenol were successfully developed in a sequencing batch reactor (SBR) using activated sludge as inoculum. Stable aerobic granules were obtained with a clearly defined shape and diameters ranging from 2 to 6 mm after 122 days of operation. The integrity coefficient (IC) and granules density was found to be 98% and 1,054 kg m−3 respectively. After development of aerobic granules, o-nitrophenols were successfully degraded to an efficiency of 78% at a concentration of 70 mg L−1. GC-MS study revealed that the biodegradation of o-nitrophenol occured via catechol via nitrobenzene pathway. Specific o-nitrophenol biodegradation rates followed the Haldane model and the associated kinetic parameters were found as follows: Vmax = 3.96 g o-nitrophenol g−1VSS−1d−1, Ks = 198.12 mg L−1, and Ki = 31.16 mg L−1. The aerobic granules proved to be a feasible and effective way to degrade o-nitrophenol containing wastewater.

The Impact of a Chemostat Discharge Containing Oil Degrading Bacteria on the Biological Kinetics of a Refinery Activated Sludge Process

1988 ◽  
Vol 20 (11-12) ◽  
pp. 131-136 ◽  
Author(s):  
A. D. Wong ◽  
C. D. Goldsmith
Keyword(s):  
Activated Sludge ◽  
Retention Time ◽  
Waste Treatment ◽  
Treatment Plant ◽  
Utilization Rate ◽  
Activated Sludge Process ◽  
Kinetic Evaluation ◽  
Degrading Bacteria ◽  
Waste Treatment Plant ◽  
The Impact

The effect of discharging specific oil degrading bacteria from a chemostat to a refinery activated sludge process was determined biokinetically. Plant data for the kinetic evaluation of the waste treatment plant was collected before and during treatment. During treatment, the 500 gallon chemostatic growth chamber was operated on an eight hour hydraulic retention time, at a neutral pH, and was fed a mixture of refinery wastewater and simple sugars. The biokinetic constants k (days−1), Ks (mg/L), and K (L/mg-day) were determined before and after treatment by Monod and Lineweaver-Burk plots. Solids discharged and effluent organic concentrations were also evaluated against the mean cell retention time (MCRT). The maximum utilization rate, k, was found to increase from 0.47 to 0.95 days−1 during the operation of the chemostat. Subsequently, Ks increased from 141 to 556 mg/L. Effluent solids were shown to increase slightly with treatment. However, this was acceptable due to the polishing pond and the benefit of increased ability to accept shock loads of oily wastewater. The reason for the increased suspended solids in the effluent was most likely due to the continual addition of bacteria in exponential growth that were capable of responding to excess substrate. The effect of the chemostatic addition of specific microbial inocula to the refinery waste treatment plant has been to improve the overall organic removal capacity along with subsequent gains in plant stability.

Enrichment and characterization of an anaerobic methyl ethyl ketoxime degrading culture from an anoxic/anaerobic/aerobic activated sludge sequencing batch reactor

1998 ◽  
Vol 37 (4-5) ◽  
pp. 95-98 ◽  
Author(s):  
Nancy G. Love ◽  
Mary E. Rust ◽  
Kathy C. Terlesky
Keyword(s):  
Energy Source ◽  
Activated Sludge ◽  
Electron Acceptor ◽  
Sequencing Batch Reactor ◽  
Enrichment Culture ◽  
Batch Reactor ◽  
Nitrification Inhibitor ◽  
Time Frame ◽  
Methyl Ethyl

An anaerobic enrichment culture was developed from an anoxic/anaerobic/aerobic activated sludge sequencing batch reactor using methyl ethyl ketoxime (MEKO), a potent nitrification inhibitor, as the sole carbon and energy source in the absence of molecular oxygen and nitrate. The enrichment culture was gradually fed decreasing amounts of biogenic organic compounds and increasing concentrations of MEKO over 23 days until the cultures metabolized the oxime as the sole carbon source; the cultures were maintained for an additional 41 days on MEKO alone. Turbidity stabilized at approximately 100 mg/l total suspended solids. Growth on selective media plates confirmed that the microorganisms were utilizing the MEKO as the sole carbon and energy source. The time frame required for growth indicated that the kinetics for MEKO degradation are slow. A batch test indicated that dissolved organic carbon decreased at a rate comparable to MEKO consumption, while sulfate was not consumed. The nature of the electron acceptor in anaerobic MEKO metabolism is unclear, but it is hypothesized that the MEKO is hydrolyzed intracellularly to form methyl ethyl ketone and hydroxylamine which serve as electron donor and electron acceptor, respectively.

On-line measurements of settling characteristics in activated sludge

1997 ◽  
Vol 36 (4) ◽  
pp. 307-312
Author(s):  
Michael R. Rasmussen ◽  
Torben Larsen
Keyword(s):  
Activated Sludge ◽  
Settling Velocity ◽  
Continuous Measurement ◽  
Batch Reactor ◽  
Volume Index ◽  
Sludge Volume Index ◽  
Dynamic Variations ◽  
On Line ◽  
Settling Column ◽  
Settling Characteristics

An on-line settling column for measuring the dynamic variations of settling velocity of activated sludge has been developed. The settling column is automatic and self-cleansing insuring continuous and reliable measurements. The settling column was tested on sludge from a batch reactor where sucrose was added as an impulse to activated sludge. The continuous measurement of settling velocity revealed a highly dynamic response after the sucrose was added. The results were verified with simultaneous measurement of the initial settling rate. A 200 hour experiment showed variations in settling velocity, which was not apparent in the DSVI (Diluted Sludge Volume Index).

GLUT2 and hexokinase control proximodistal gradient of intestinal glucose metabolism in the newborn pig

1997 ◽  
Vol 272 (6) ◽  
pp. G1530-G1539 ◽  
Author(s):  
C. Cherbuy ◽  
B. Darcy-Vrillon ◽  
L. Posho ◽  
P. Vaugelade ◽  
M. T. Morel ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Glucose Transporter ◽  
Glucose Utilization ◽  
Specific Effect ◽  
Glucose Consumption ◽  
Utilization Rate ◽  
Glucose Turnover ◽  
Proximal Jejunum ◽  
A Site

We have reported previously that a high glycolytic capacity develops soon after birth in enterocytes isolated from suckling newborn pigs. In the present work, we investigated whether such metabolic changes could affect intestinal glucose utilization in vivo and examined possible variations in glucose metabolism along the small intestine. Glucose utilization by individual tissues was assessed using the 2-deoxyglucose technique. The overall glucose utilization rate was doubled in suckling vs. fasting 2-day-old pigs because of significantly higher rates in all tissues studied, except for the brain. In parallel, enterocytes were isolated from the proximal, medium, or distal jejunoileum of newborn vs. 2-day-old pigs and assessed for their capacity to utilize, transport, and phosphorylate glucose. Intestinal glucose consumption accounted for approximately 15% of glucose turnover rate in suckling vs. 8% in fasting pigs. Moreover, there was a proximal-to-distal gradient of glucose utilization in the intestinal mucosa of suckling pigs. Such a gradient was also evidenced on isolated enterocytes. The stimulation of both hexokinase activity (HK2 isoform) and basolateral glucose transporter (GLUT2), as observed in the proximal jejunum, could account for such a site-specific effect of suckling.

Excess nitrogen accumulation in activated sludge in sequencing batch reactor with a single-stage oxic process

2009 ◽  
Vol 59 (3) ◽  
pp. 573-582 ◽  
Author(s):  
Xiao-ming Li ◽  
Dong-bo Wang ◽  
Qi Yang ◽  
Wei Zheng ◽  
Jian-bin Cao ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Sequencing Batch Reactor ◽  
Ph Value ◽  
Batch Reactor ◽  
Nitrogen Loss ◽  
Single Stage ◽  
Synthetic Wastewater ◽  
Nitrogen Accumulation ◽  
Excess Nitrogen

It was occasionally found that a significant nitrogen loss in solution under neutral pH value in a sequencing batch reactor with a single-stage oxic process using synthetic wastewater, and then further studies were to verify the phenomenon of nitrogen loss and to investigate the pathway of nitrogen removal. The result showed that good performance of nitrogen removal was obtained in system. 0–7.28 mg L−1 ammonia, 0.08–0.38 mg L−1 nitrite and 0.94–2.12 mg L−1 nitrate were determined in effluent, respectively, when 29.85–35.65 mg L−1 ammonia was feeding as the sole nitrogen source in influent. Furthermore, a substantial nitrogen loss in solution (95% of nitrogen influent) coupled with a little gaseous nitrogen increase in off-gas (7% of nitrogen influent) was determined during a typical aerobic phase. In addition, about 322 mg nitrogen accumulation (84% of nitrogen influent) was detected in activated sludge. Based on nitrogen mass balance calculation, the unaccounted nitrogen fraction and the ratio of nitrogen accumulation in sludge/nitrogen loss in solution were 14.6 mg (3.7% of nitrogen influent) and 0.89, respectively. The facts indicated that the essential pathway of nitrogen loss in solution in this study was excess nitrogen accumulation in activated sludge.

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