Biological phosphorus removal processes - effect of pH on anaerobic substrate metabolism

1996 ◽  
Vol 34 (1-2) ◽  
pp. 25-32 ◽  
Author(s):  
Liu Wen-Tso ◽  
Takashi Mino ◽  
Tomonori Matsuo ◽  
Kazunori Nakamura

Effect of pH on the anaerobic acetate metabolism of biological phosphorus (P) removal processes was determined using anaerobic-aerobic activated sludge cultured with mainly acetate and containing a 12% or an 8% of total biomass as P (Px). The acetate uptake coupling to phosphate (Pi) release was found to be kinetically and thermodynamically influenced by pH. In the pH range 5.0–6.5, the acetate uptake rate (AUR) increased linearly with pH from 0 to about 50 (mg C/g VSS/h), and the Pi release rate (PiRR) from 20 to about 50 (mg P/g VSS/h). In the pH range 6.5–8.0, AUR remained at a constant range but PiRR continuously increased. Above pH 8.0, both AUR and PiRR started to decrease. With regard to the molar ratio of Pi released per acetate taken up (Pi/Ac), it was about 1.0 or 0.70–0.75 in the pH range 5.5–6.5, and proportionally increased to 1.75 or 1.50 in the pH range 6.6–8.5 for sludge containing a 12% Px or an 8% Px, respectively. Apparently, acidic pH inactivated the acetate metabolism, and basic pH stimulated too much Pi release, resulting an increase in energy consumption for acetate uptake (i.e. the Pi/Ac ratio). As a compromise, an optimum pH 6.8±0.7 was proposed for anaerobic acetate metabolism, because that a relatively high AUR with less energy consumption can be maintained by the bacteria that respond for biological P removal.

1985 ◽  
Vol 17 (11-12) ◽  
pp. 147-162 ◽  
Author(s):  
E. Arvin ◽  
G. Holm Kristensen

The stripping of phosphorus, potassium and magnesium, and uptake of organic substrate and calcium was studied in the anaerobic reactors of two biological phosphorus and nitrogen removal plants. Most of the experiments were performed in batch systems simulating the function of the anaerobic reactor. Acetate-, propionate- and lactate addition to the sludge under anaerobic conditions resulted in a significant P-release. The P-release with glucose was significantly smaller. Approximate values for the ∆ P/∆COD ratios were estimated. Average ∆ K/ ∆P and ∆Mg/ ∆P values were estimated to about 0.29 and 0.25 mg/mg, respectively. Phosphate appears to be precipitated simultaneously with the biological P-stripping. In the plant under detailed investigations, about 60% of the total phosphate is precipitated, irrespective of the total P-content in the sludge. Calcium and magnesium are the dominating counterions, the (Ca+Mg)/P molar ratio being about 1.1. Some iron appears also to be associated with the phosphate, 0.6% of the dry sludge.


2017 ◽  
Vol 77 (5) ◽  
pp. 1222-1229 ◽  
Author(s):  
Jie Fan ◽  
Hang Zhang ◽  
Jiasong Ye ◽  
Bin Ji

Abstract In simultaneous precipitation, interaction between chemical and biological P removal could not be ignored. This work investigated effects of ferrous sulfate and Fe precipitates on metabolic behavior of bio-P and its counter cation of potassium. After dosing, mixed liquid suspended solids (MLSS) increased 9%, pH decreased from 7.35 to 7.00, sludge volume index (SVI) decreased, electrical conductivity increased. Chemical oxygen demand (COD) and NH3 removal was not affected. Fe dosing initially showed synergistic effect, and then inhibition appeared at accumulative dose above 10 mgFe/gMLSS. Both precipitate FePO4 and Fe(OH)3 deteriorated effluent P. FePO4 dissolved 35% in anaerobic phase which failed to be totally reprecipitated in oxic phase, resulting in increased effluent P. FePO4 inhibited K uptake rather than bio-P uptake. Fe(OH)3 caused reduction of bio-P release, meanwhile, its inhibition on K and bio-P uptake was greater than FePO4. Phosphorus metabolism was inhibited when sludge contained 0.15 mM FePO4 or 0.10 mM Fe(OH)3. Increased K/P molar ratio and coefficient b could be indicators for Fe residual in sludge. Intermittent dosing was suggested for wastewater treatment plant (WWTP) operation.


1985 ◽  
Vol 17 (11-12) ◽  
pp. 297-298 ◽  
Author(s):  
Takao Murakami ◽  
Atsushi Miyairi ◽  
Kazuhiro Tanaka

In Japan various biological phosphorus removal processes have recently been researched by laboratory or pilot plant scale studies and most of them have shown good results. Based on these results, the Japan Sewage Works Agency has conducted a full scale study of the biological phosphorus removal process from June 1982 until February 1983, which was the first full scale operation of this process in Japan. The main purpose of the study was to evaluate phosphorus removal efficiency and also nitrogen removal efficiency of the process and in addition, to ascertain the important operating factors of the process. For the study a treatment train of a large scale sewage treatment plant was remodelled. The aeration tank of 3.825 m3 volume was divided into four equal cells. The whole train including return sludge line was operated entirely independently of the other trains. During the experiment the train was operated under two different modes, Mode 1 and Mode 2. In Mode 1, the train was operated as an A/O process, the first cell of the aeration tank being anaerobic and the other cells oxic. In Mode 2, the train was operated as a Modified Phoredox process. In this case, the first cell was anaerobic, but the second cell was anoxic and nitrified liquor was returned to it from the end of the oxic cells. Mode 1 and Mode 2 were further divided into many ‘runs' and the flow rate varied between 12,550 m3 d−1 and 25,270 m3 d−1 , corresponding to retention times of 7.3 hours and 3.6 hours, respectively. Throughout the experimental period the mean value of influent (primary effluent) total-P concentration was 3.38 mg 1−1 , and that of the final effluent was 0.47 mg 1−1 . A cumulated frequency curve of the data showed that about 93% of measured effluent total-P was below 1.0 mg l−1 . Therefore, it can be concluded that with these influent total-P levels, biological phosphorus removal processes can sufficiently satisfy the effluent standard of 1 mg 1−1 total-P. Even when the process was operated as a Modified Phoredox Process, no obstruction to phosphorus removal because of nitrification was observed and phosphorus removal remained good. However, since the sewage treatment plant treated influent from a combined sewerage system, phosphorus removal was sometimes affected by heavy rainfalls. In such cases phosphorus release in the anaerobic cell was insufficient because of increased influent NOx concentration and accordingly increased denitrification level in the anaerobic cell. Therefore, as a result, enhanced phosphorus uptake in the following cells could not be observed. Higher process stability can be expected if an effective countermeasure to high influent NOx concentration can be made. Influence of flow rate fluctuation on the process was also studied. The treatment train was operated for a week under a daily flow rate fluctuation pattern which ranged between 460 m3 hr−1 and 820 m3 hr−1 . Nevertheless, the effluent total-P concentration showed no increase and stayed constantly lower than 0.5 mg 1−1. The oxidation reduction potential (ORP) was an effective control index to evaluate the degree of phosphorus release in the anaerobic cell. Water temperature did not affect phosphorus release and uptake rates.


1994 ◽  
Vol 29 (7) ◽  
pp. 153-156 ◽  
Author(s):  
D. Wedi ◽  
P. A. Wilderer

Most of the fundamental processes responsible for enhanced biological phosphorus removal (EBPR) were obtained through laboratory tests under defined conditions with pure or enriched cultures. Acinetobacter sp. was identified as the most important group of bacteria responsible for bio-P removal. Full scale data showed, however, that laboratory results do not match full scale results well enough. There is a lack of data on the effects of sub-optimal process conditions such as inadequate availability of volatile fatty acids (VFA), high nitrate recycle, storm water inflow or low temperatures. In this paper the results of full scale experiments on P-release are presented and compared with theoretical values. Measurements at a full scale Phoredox-system showed a surprisingly low P-release in the anaerobic reactor. Only 4 to 10% of the phosphorus in the activated sludge was released in the bulk liquid. With laboratory batch-tests, a maximum of 20% of the P in the sludge could be released. It is assumed that under the prevailing process conditions either the fraction of Acinetobacter sp. was very small, or bacteria other than Acinetobacter sp. were responsible for the P-removal, or most of the phosphorus was bound chemically but mediated by biological processes.


1999 ◽  
Vol 39 (6) ◽  
pp. 13-20 ◽  
Author(s):  
Philip L. Bond ◽  
Jürg Keller ◽  
Linda L. Blackall

Culturing bacteria from activated sludge with enhanced biological phosphorus removal (EBPR) has strongly implicated Acinetobacter with the process. However, using fluorescent in-situ hybridisation (FISH) probing to analyse microbial populations, we have shown evidence opposing this widespread belief. We describe the phosphorus (P) removing performance and microbial population analyses of sludges obtained in a laboratory scale EBPR reactor. Two sludges with extremely high P removing capabilities were examined, the P content of these sludges was 8.6% (P sludge) and 12.3% (S sludge) of the MLSS. Identification of bacteria using FISH probing indicated both sludges were dominated by microbes from the beta proteobacteria and high mol% G+C Gram positive bacteria. Acinetobacter could make up only a small proportion of the cells in these sludges. Sludge with extremely poor P removal (P content of 1.5%, referred to as T sludge) was then generated by reducing the P in the influent. Bacteria resembling the G-bacteria became abundant in this sludge and these were identified using FISH probing. The anaerobic transformations of the T and P sludges correlated well with that of the non-EBPR and EBPR biological models respectively, indicating that bacteria in the T sludge have the potential to inhibit P removal in EBPR systems.


2003 ◽  
Vol 48 (1) ◽  
pp. 87-94 ◽  
Author(s):  
B. Lesjean ◽  
R. Gnirss ◽  
C. Adam ◽  
M. Kraume ◽  
F. Luck

The enhanced biological phosphorus removal (EBPR) process was adapted to membrane bioreactor (MBR) technology. One bench-scale plant (BSP, 200-250 L) and two pilot plants (PPs, 1,000-3,000 L each) were operated under several configurations, including pre-denitrification and post-denitrification without addition of carbon source, and two solid retention times (SRT) of 15 and 26 d. The trials showed that efficient Bio-P removal can be achieved with MBR systems, in both pre- and post-denitrification configurations. EBPR dynamics could be clearly demonstrated through batch-tests, on-line measurements, profile analyses, P-spiking trials, and mass balances. High P-removal performances were achieved even with high SRT of 26 d, as around 9 mgP/L could be reliably removed. After stabilisation, the sludge exhibited phosphorus contents of around 2.4%TS. When spiked with phosphorus (no P-limitation), P-content could increase up to 6%TS. The sludge is therefore well suited to agricultural reuse with important fertilising values. Theoretical calculations showed that increased sludge age should result in a greater P-content. This could not be clearly demonstrated by the trials. This effect should be all the more significant as the influent is low in suspended solids.


2006 ◽  
Vol 95 (5) ◽  
pp. 875-882 ◽  
Author(s):  
J. Serralta ◽  
J. Ferrer ◽  
L. Borrás ◽  
A. Seco

Chemosphere ◽  
2019 ◽  
Vol 218 ◽  
pp. 670-679 ◽  
Author(s):  
Zidan Yuan ◽  
Guoqing Zhang ◽  
Jinru Lin ◽  
Xiangfeng Zeng ◽  
Xu Ma ◽  
...  

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