scholarly journals Anammox Bacteria Are Potentially Involved in Anaerobic Ammonium Oxidation Coupled to Iron(III) Reduction in the Wastewater Treatment System

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao-Ru Yang ◽  
Hu Li ◽  
Jian-Qiang Su ◽  
Guo-Wei Zhou
Keyword(s):  
Wastewater Treatment ◽  
Amplicon Sequencing ◽  
Anaerobic Ammonium Oxidation ◽  
Nitrite Reduction ◽  
Treatment System ◽  
Anammox Bacteria ◽  
Rrna Gene ◽  
Ammonium Oxidation ◽  
Wastewater Treatment System ◽  
Relative Abundances

Anaerobic ammonium oxidation coupled to nitrite reduction (termed as Anammox) was demonstrated as an efficient pathway to remove nitrogen from a wastewater treatment system. Recently, anaerobic ammonium oxidation was also identified to be linked to iron(III) reduction (termed Feammox) with dinitrogen, nitrite, or nitrate as end-product, reporting to enhance nitrogen removal from the wastewater treatment system. However, little is known about the role of Anammox bacteria in the Feammox process. Here, slurry from wastewater reactor amended with ferrihydrite was employed to investigate activity of Anammox bacteria in the Feammox process using the 15N isotopic tracing technique combined with 16S rRNA gene amplicon sequencing. A significantly positive relationship between rates of 15N2 production and iron(III) reduction indicated the occurrence of Feammox during incubation. Relative abundances of Anammox bacteria including Brocadia, Kuenenia, Jettenia, and unclassified Brocadiaceae were detected with low relative abundances, whereas Geobacteraceae dominated in the treatment throughout the incubation. 15N2 production rates significantly positively correlated with relative abundances of Geobacter, unclassified Geobacteraceae, and Anammox bacteria, revealing their contribution to nitrogen generation via Feammox. Overall, these findings suggested Anammox bacteria or cooperation between Anammox bacteria and iron(III) reducers serves a potential role in Feammox process.

Investigation of aerobic and anaerobic ammonium-oxidising bacteria presence in a small full-scale wastewater treatment system comprised by UASB reactor and three polishing ponds

2010 ◽  
Vol 61 (3) ◽  
pp. 737-743 ◽  
Author(s):  
J. C. Araujo ◽  
M. M. S. Correa ◽  
E. C. Silva ◽  
A. P. Campos ◽  
V. M. Godinho ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Ammonia Removal ◽  
Treatment System ◽  
Nitrifying Bacteria ◽  
Uasb Reactor ◽  
Anammox Bacteria ◽  
Fluorescence In Situ Hybridisation ◽  
Probable Number ◽  
Sediment Samples ◽  
Wastewater Treatment System

This work applied PCR amplification method and Fluorescence in situ hybridisation (FISH) with primers and probes specific for the anammox organisms and aerobic ammonia-oxidising β-Proteobacteria in order to detect these groups in different samples from a wastewater treatment system comprised by UASB reactor and three polishing (maturation) ponds in series. Seven primer pairs were used in order to detect Anammox bacteria. Positive results were obtained with three of them, suggesting that Anammox could be present in polishing pond sediments. However, Anammox bacteria were not detected by FISH, indicating that they were not present in sediment samples, or they could be present but below FISH detection limit. Aerobic ammonia- and nitrite-oxidising bacteria were verified in water column samples through Most Probable Number (MPN) analysis, but they were not detected in sediment samples by FISH. Ammonia removal efficiencies occurred systematically along the ponds (24, 32, and 34% for polishing pond 1, 2, and 3, respectively) but the major reaction responsible for this removal is still unclear. Some nitrification might have occurred in water samples because some nitrifying bacteria were present. Also Anammox reaction might have occurred because Anammox genes were detected in the sediments, but probably this reaction was too low to be noticed. It is important also to consider that some of the ammonia removal observed might be related to NH3 stripping, associated with the pH increase resulting from the intensive photosynthetic activity in the ponds (mechanism under investigation). Therefore, it can be concluded that more than one mechanism (or reaction) might be involved in the ammonia removal in the polishing ponds investigated in this study.

Activities and Metabolic Versatility of Distinct Anammox Bacteria in a Full-Scale Wastewater Treatment System

2021 ◽  
pp. 117763
Author(s):  
Yuchun Yang ◽  
Mohammad Azari ◽  
Craig W. Herbold ◽  
Meng Li ◽  
Huaihai Chen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Full Scale ◽  
Treatment System ◽  
Anammox Bacteria ◽  
Wastewater Treatment System ◽  
Metabolic Versatility

Study on Aerobic Biological Phase in the System of Coal Tar Processing Wastewater Treatment

2011 ◽  
Vol 108 ◽  
pp. 183-188 ◽  
Author(s):  
Yan Li Lǚ ◽  
Yan Qiu Wang ◽  
Ming Jun Shan ◽  
Da Wei Pan ◽  
Tie Feng Li ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Biological Treatment ◽  
Coal Tar ◽  
Reactor System ◽  
Anaerobic Ammonium Oxidation ◽  
Treatment System ◽  
Ammonium Oxidation ◽  
Ammonium Removal ◽  
Dirty Water ◽  
Biological Treatment System

The paper discusses the change regulation, characteristics and designation function of the aerobic biological phase in the biological treatment system of single reactor system for high ammonium removal over nitrite-anaerobic ammonium oxidation (SHARON-ANAMMOX) of Coal Tar Processing wastewater treatment. And soma advice is given for the adjustment and supervision of the similar biological disposing system of industrial dirty water.

scholarly journals Burkholderia zhejiangensis sp. nov., a methyl-parathion-degrading bacterium isolated from a wastewater-treatment system

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1337-1341 ◽  
Author(s):  
Peng Lu ◽  
Liu-Qiang Zheng ◽  
Jin-Jin Sun ◽  
Hong-Ming Liu ◽  
Shun-Peng Li ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Type Species ◽  
Methyl Parathion ◽  
Treatment System ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Wastewater Treatment System ◽  
Link Type ◽  
Degrading Bacterium

The taxonomic status of a methyl-parathion-degrading strain, OP-1T, isolated from a wastewater-treatment system in China, was determined using a polyphasic approach. The rod-shaped cells were Gram-staining-negative, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain belonged to the genus Burkholderia , as it appeared closely related to Burkholderia glathei ATCC 29195T (97.4 % sequence similarity), Burkholderia sordidicola KCTC 12081T (96.5 %) and Burkholderia bryophila LMG 23644T (96.3 %). The major cellular fatty acids, C16 : 0, C17 : 0 cyclo and C18 : 1ω7c, were also similar to those found in established members of the genus Burkholderia . The genomic DNA G+C content of strain OP-1T was 59.4 mol%. The level of DNA–DNA relatedness between the novel strain and the closest recognized species, Burkholderia glathei ATCC 29195T, was only 30 %. Based on the phenotypic, genotypic and phylogenetic evidence, strain OP-1T represents a novel species of the genus Burkholderia , for which the name Burkholderia zhejiangensis sp. nov. is proposed. The type strain is OP-1T ( = CCTCC AB 2010354T = KCTC 23300T).

16S rRNA gene-based primer pair showed high specificity and quantification accuracy in detecting freshwater Brocadiales anammox bacteria

2020 ◽  
Vol 96 (3) ◽  
Author(s):  
Chang Ding ◽  
Lorenz Adrian ◽  
Yongzhen Peng ◽  
Jianzhong He
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wastewater Treatment Plants ◽  
High Specificity ◽  
Amplicon Sequencing ◽  
Anammox Bacteria ◽  
Rrna Gene ◽  
Detection And Quantification

ABSTRACT Anaerobic ammonium oxidizing (anammox) bacteria are widely distributed and contribute significantly to the global nitrogen cycle. Traditionally, identification and quantification based on the 16S rRNA gene were considered not reliable because of low 16S rRNA gene sequence identity within Brocadiales. Here we hypothesize that by using appropriate primers and methodology, 16S-based detection and quantification of anammox bacteria can be accurate. We modified an existing 16S rRNA gene-based primer pair (Amx694F–Amx960R) by changing one nucleotide (Amx694F position 18, G→C) (Amx694PF–Amx960R) so that they match the sequences of most Brocadiales anammox bacteria, and evaluated the modified primer pair with 29 freshwater samples from microcosms, anammox reactors and wastewater treatment plants of various geographical origins. The primer pair showed high specificity in detection and quantification of anammox populations in samples that contained >0.1% anammox bacteria. Quantification of anammox abundance by quantitative real-time PCR and delineation of anammox species by denaturing gradient gel electrophoresis agreed well with amplicon sequencing results. A clear shift of anammox population towards ‘Candidatus Kuenenia’ was observed under laboratory cultivation conditions. With the help of amplicon sequencing, we demonstrated that 16S rRNA gene-based anammox-specific primers are able to achieve qualitative and quantitative monitoring of anammox communities in wastewater treatment plants and natural freshwater environments.2007;73:5261–7.

scholarly journals Diversity and functional prediction of microbial communities involved in the first aerobic bioreactor of coking wastewater treatment system

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243748
Author(s):  
Jinsi Deng ◽  
Baoshan Zhang ◽  
Junting Xie ◽  
Haizhen Wu ◽  
Zemin Li ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Activated Sludge ◽  
Removal Rate ◽  
Treatment System ◽  
Rrna Gene ◽  
Coking Wastewater ◽  
Microbial Composition ◽  
Term Operation ◽  
Wastewater Treatment System

The pre-aerobic process of coking wastewater treatment has strong capacity of decarbonization and detoxification, which contribute to the subsequent dinitrogen of non-carbon source/heterotrophic denitrification. The COD removal rate can reach > 90% in the first aerobic bioreactor of the novel O/H/O coking wastewater treatment system during long-term operation. The physico-chemical characteristics of influent and effluent coking wastewater in the first aerobic bioreactor were analyzed to examine how they correlated with bacterial communities. The diversity of the activated sludge microbial community was investigated using a culture-independent molecular approach. The microbial community functional profiling and detailed pathways were predicted from the 16S rRNA gene-sequencing data by the PICRUSt software and the KEGG database. High-throughput MiSeq sequencing results revealed a distinct microbial composition in the activated sludge of the first aerobic bioreactor of the O/H/O system. Proteobacteria, Bacteroidetes, and Chlorobi were the decarbonization and detoxification dominant phyla with the relative abundance of 84.07 ± 5.45, 10.89 ± 6.31, and 2.96 ± 1.12%, respectively. Thiobacillus, Rhodoplanes, Lysobacter, and Leucobacter were the potential major genera involved in the crucial functional pathways related to the degradation of phenols, cyanide, benzoate, and naphthalene. These results indicated that the comprehensive understanding of the structure and function diversity of the microbial community in the bioreactor will be conducive to the optimal coking wastewater treatment.

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