Revealing biogenic sulfuric acid corrosion in sludge digesters: detection of sulfur-oxidizing bacteria within full-scale digesters

2014 ◽  
Vol 70 (8) ◽  
pp. 1405-1411 ◽  
Author(s):  
B. Huber ◽  
J. E. Drewes ◽  
K. C. Lin ◽  
R. König ◽  
E. Müller
Keyword(s):  
Sulfuric Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Acid Corrosion ◽  
Full Scale ◽  
Acidithiobacillus Thiooxidans ◽  
Thiobacillus Thioparus ◽  
Gradient Gel Electrophoresis ◽  
Sulfuric Acid Corrosion ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

Biogenic sulfuric acid corrosion (BSA) is a costly problem affecting both sewerage infrastructure and sludge handling facilities such as digesters. The aim of this study was to verify BSA in full-scale digesters by identifying the microorganisms involved in the concrete corrosion process, that is, sulfate-reducing (SRB) and sulfur-oxidizing bacteria (SOB). To investigate the SRB and SOB communities, digester sludge and biofilm samples were collected. SRB diversity within digester sludge was studied by applying polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the dsrB-gene (dissimilatory sulfite reductase beta subunit). To reveal SOB diversity, cultivation dependent and independent techniques were applied. The SRB diversity studies revealed different uncultured SRB, confirming SRB activity and H2S production. Comparable DGGE profiles were obtained from the different sludges, demonstrating the presence of similar SRB species. By cultivation, three pure SOB strains from the digester headspace were obtained including Acidithiobacillus thiooxidans, Thiomonas intermedia and Thiomonas perometabolis. These organisms were also detected with PCR-DGGE in addition to two new SOB: Thiobacillus thioparus and Paracoccus solventivorans. The SRB and SOB responsible for BSA were identified within five different digesters, demonstrating that BSA is a problem occurring not only in sewer systems but also in sludge digesters. In addition, the presence of different SOB species was successfully associated with the progression of microbial corrosion.

scholarly journals Characterization of sulfur oxidizing bacteria related to biogenic sulfuric acid corrosion in sludge digesters

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Bettina Huber ◽  
Bastian Herzog ◽  
Jörg E. Drewes ◽  
Konrad Koch ◽  
Elisabeth Müller
Keyword(s):  
Sulfuric Acid ◽  
Acid Corrosion ◽  
Sulfuric Acid Corrosion ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

scholarly journals Succession of Sulfur-Oxidizing Bacteria in the Microbial Community on Corroding Concrete in Sewer Systems

2006 ◽  
Vol 73 (3) ◽  
pp. 971-980 ◽  
Author(s):  
Satoshi Okabe ◽  
Mitsunori Odagiri ◽  
Tsukasa Ito ◽  
Hisashi Satoh
Keyword(s):  
Sulfuric Acid ◽  
Microbial Community ◽  
Concrete Surface ◽  
Molecular Techniques ◽  
Rrna Gene ◽  
Acidithiobacillus Thiooxidans ◽  
Community Members ◽  
Sewer Systems ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

ABSTRACT Microbially induced concrete corrosion (MICC) in sewer systems has been a serious problem for a long time. A better understanding of the succession of microbial community members responsible for the production of sulfuric acid is essential for the efficient control of MICC. In this study, the succession of sulfur-oxidizing bacteria (SOB) in the bacterial community on corroding concrete in a sewer system in situ was investigated over 1 year by culture-independent 16S rRNA gene-based molecular techniques. Results revealed that at least six phylotypes of SOB species were involved in the MICC process, and the predominant SOB species shifted in the following order: Thiothrix sp., Thiobacillus plumbophilus, Thiomonas intermedia, Halothiobacillus neapolitanus, Acidiphilium acidophilum, and Acidithiobacillus thiooxidans. A. thiooxidans, a hyperacidophilic SOB, was the most dominant (accounting for 70% of EUB338-mixed probe-hybridized cells) in the heavily corroded concrete after 1 year. This succession of SOB species could be dependent on the pH of the concrete surface as well as on trophic properties (e.g., autotrophic or mixotrophic) and on the ability of the SOB to utilize different sulfur compounds (e.g., H2S, S0, and S2O3 2−). In addition, diverse heterotrophic bacterial species (e.g., halo-tolerant, neutrophilic, and acidophilic bacteria) were associated with these SOB. The microbial succession of these microorganisms was involved in the colonization of the concrete and the production of sulfuric acid. Furthermore, the vertical distribution of microbial community members revealed that A. thiooxidans was the most dominant throughout the heavily corroded concrete (gypsum) layer and that A. thiooxidans was most abundant at the highest surface (1.5-mm) layer and decreased logarithmically with depth because of oxygen and H2S transport limitations. This suggested that the production of sulfuric acid by A. thiooxidans occurred mainly on the concrete surface and the sulfuric acid produced penetrated through the corroded concrete layer and reacted with the sound concrete below.

scholarly journals Novel Approaches for Biocorrosion Mitigation in Sewer Systems

Chemistry ◽  
2021 ◽  
Vol 3 (4) ◽  
pp. 1166-1177
Author(s):  
Georgios Fytianos ◽  
Dimitra Banti ◽  
Esmeralda Dushku ◽  
Efthimios Papastergiadis ◽  
Minas Yiangou ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Tio2 Nanoparticles ◽  
Acidithiobacillus Thiooxidans ◽  
Sewer Pipes ◽  
Sewer Systems ◽  
Sulfur Oxidizing ◽  
Novel Approaches ◽  
Potential Inhibitors ◽  
Microbiologically Induced Corrosion ◽  
Sulfur Oxidizing Bacteria

Concrete sewer pipes can be corroded by the biogenic sulfuric acid (H2SO4) generated from microbiological activities in a process called biocorrosion or microbiologically induced corrosion (MIC). In this study, inhibitors that can reduce Acidithiobacillus thiooxidans growth and thus may reduce the accumulation of biofilm components responsible for the biodegradation of concrete were used. D-tyrosine, tetrakis hydroxymethyl phosphonium sulfate (THPS) and TiO2 nanoparticles were investigated as potential inhibitors of sulfur-oxidizing bacteria (SOB) growth. Results showed that most of the chemicals used can inhibit SOB growth at a concentration lower than 100 mg/L. TiO2 nanoparticles exhibited the highest biocide effect and potential biocorrosion mitigation activity, followed by D-tyrosine and THPS.

scholarly journals Resistance to Sulfuric Acid Corrosion of Geopolymer Concrete Based on Different Binding Materials and Alkali Concentrations

Materials ◽  
2021 ◽  
Vol 14 (23) ◽  
pp. 7109
Author(s):  
Wei Yang ◽  
Pinghua Zhu ◽  
Hui Liu ◽  
Xinjie Wang ◽  
Wei Ge ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Corrosion Resistance ◽  
Sulfuric Acid ◽  
Fly Ash ◽  
Acid Corrosion ◽  
Geopolymer Concrete ◽  
High Calcium ◽  
High Calcium Fly Ash ◽  
Naoh Solution ◽  
Sulfuric Acid Corrosion

Geopolymer binder is expected to be an optimum alternative to Portland cement due to its excellent engineering properties of high strength, acid corrosion resistance, low permeability, good chemical resistance, and excellent fire resistance. To study the sulfuric acid corrosion resistance of geopolymer concrete (GPC) with different binding materials and concentrations of sodium hydroxide solution (NaOH), metakaolin, high-calcium fly ash, and low-calcium fly ash were chosen as binding materials of GPC for the geopolymerization process. A mixture of sodium silicate solution (Na2SiO3) and NaOH solution with different concentrations (8 M and 12 M) was selected as the alkaline activator with a ratio (Na2SiO3/NaOH) of 1.5. GPC specimens were immersed in the sulfuric acid solution with the pH value of 1 for 6 days and then naturally dried for 1 day until 98 days. The macroscopic properties of GPC were characterized by visual appearance, compressive strength, mass loss, and neutralization depth. The materials were characterized by SEM, XRD, and FTIR. The results indicated that at the immersion time of 28 d, the compressive strength of two types of fly ash-based GPC increased to some extent due to the presence of gypsum, but this phenomenon was not observed in metakaolin-based GPC. After 98 d of immersion, the residual strength of fly ash based GPC was still higher, which reached more than 25 MPa, while the metakaolin-based GPC failed. Furthermore, due to the rigid 3D networks of aluminosilicate in fly ash-based GPC, the mass of all GPC decreased slightly during the immersion period, and then tended to be stable in the later period. On the contrary, in metakaolin-based GPC, the incomplete geopolymerization led to the compressive strength being too low to meet the application of practical engineering. In addition, the compressive strength of GPC activated by 12 M NaOH was higher than the GPC activated by 8 M NaOH, which is owing to the formation of gel depended on the concentration of alkali OH ion, low NaOH concentration weakened chemical reaction, and reduced compressive strength. Additionally, according to the testing results of neutralization depth, the neutralization depth of high-calcium fly ash-based GPC activated by 12 M NaOH suffered acid attack for 98 d was only 6.9 mm, which is the minimum value. Therefore, the best performance was observed in GPC prepared with high-calcium fly ash and 12 M NaOH solution, which is attributed to gypsum crystals that block the pores of the specimen and improve the microstructure of GPC, inhibiting further corrosion of sulfuric acid.

Biogenic sulfuric acid corrosion resistance of new artificial reef concrete

2018 ◽  
Vol 158 ◽  
pp. 33-41 ◽  
Author(s):  
Yu Yang ◽  
Tao Ji ◽  
Xujian Lin ◽  
Caiyi Chen ◽  
Zhengxian Yang
Keyword(s):  
Corrosion Resistance ◽  
Sulfuric Acid ◽  
Acid Corrosion ◽  
Artificial Reef ◽  
Sulfuric Acid Corrosion

Experiment Study on Degradation Laws of Sulfuric Acid Corrosion Reinforcements

2018 ◽  
Vol 878 ◽  
pp. 23-27 ◽  
Author(s):  
Ming Qiang Lin ◽  
Feng Juan Dai ◽  
Jia Tao Li
Keyword(s):  
Sulfuric Acid ◽  
Acid Corrosion ◽  
Constitutive Models ◽  
Corrosion Damage ◽  
Uniform Corrosion ◽  
Steel Bars ◽  
Relationship Model ◽  
Strain Relationship ◽  
Sulfuric Acid Corrosion ◽  
Corrosion Of Steel

The corrosion of concrete structures is serious in sulfuric acid environments. Corrosion damage of reinforcements caused sulfuric acid corrosion is very serious. The rapid experiments of sulfuric acid corrosion steel bars were carried out, and the apparent morphology and mechanical properties of sulfuric acid corrosion steel bars were studied. The results show that the corrosion of steel bars is uniform corrosion. With the increase of corrosion rate, the yield platforms and the yield strengths and ultimate strengths are reduced. Based on the experimental datas, the relationship models between yield strengths and ultimate strengths and corrosion rates were obtained. The constitutive models of corrosion steel bars were established. The stress - strain relationship model is in good agreement with the experimental data.

Sign in / Sign up

Export Citation Format

Share Document