Effect of Sulfur-Oxidizing Bacteria Thiobacillus Thioparus and Different Levels of Agricultural Sulfur on Wheat Yield (Triticum Aestivium L.)

A field experiment was carried out in the agricultural season 2020-2021 in an agricultural field located in the Al-Jarboua area (Al Hafez) in Al-Muthanna Governorate to study the effect of inoculation with sulfur-oxidizing bacteria and agricultural sulfur levels and their interactions on the yield of wheat plant Triticum aestivum L. Ibaa cultivar 99. The study was carried out in two stages, the first is isolation Sulfur oxidizing bacteria and the second used as a inoculant in the agricultural experiment to study its effect on soil characteristics and its content of nutrients NPK and S. At two levels T0 without adding the bacterial inoculum and T1 adding the first isolate of bacteria The second factor included the addition of agricultural sulfur with four levels of sulfur (S0 were taken without addition, S1 added 750 kg ha−1, S2 added 1500 kg ha−1 and S3 added 2250 kg ha−1) In three replications, the number of experimental units reached 36 experimental units. The results of the study also showed that inoculation with bacterial isolates led to a significant increase in the readiness of nutrients N, P, K and S, and the highest rate of sulfur was (1895) mg. The pollination also led to a significant increase in the growth characteristics of the plant (plant height, number of spikes and grain yield), as it recorded (101.25 cm, 393.22 spike m−1 and 6.73 mcg/ha−1), respectively. The addition of agricultural sulfur at different levels affected a significant increase in the availability of nutrients N, P, K and S, and the highest rate of sulfur at the level of S3 was (1817) mg. Also, the bilateral interaction between inoculation with bacterial isolates and agricultural sulfur led to a significant increase in the availability of nutrients N, P, K and S. kg −1 soil.

Isolation and Characterization of Halophilic and Halotolerant Bacteria from Urmia Lake after the Recent Drought Disaster in 2015

Background: Due to the last two decades of drought disaster, which resulted in the loss of the main part of Urmia Lake water and changed the natural conditions of an environment, especially ionic strength. Objective: We aimed to isolate and characterize halophilic and halotolerant bacteria in Urmia Lake, Iran, 2015. Urmia Lake is a permanent and salty inland lake located in the Azerbaijan region in northwestern Iran. Methods: Sampling was carried out in multiple water-filled locations of the lake. Liquid basal media for the enrichment of bacteria was successively applied and colonies were isolated by the plating method. Isolates were then distinguished based on differences in colony, Gram staining, microscopic shape, and biochemical properties. Results: One chemolithotrophic isolate belonging to Thiobacillus thioparus and 41 heterotrophic isolates were obtained. The 16S rRNA gene sequences showed that all 42 isolates belong to the genera Kocuria (21.42%), Marinobacter (11.90%), Micrococcus (11.90%), Thalassobacillus (11.90%), Bacillus (11.90%), Halomonas (7.14%) and Thiobacillus (2.38%). Conclusion: Based on 16S rRNA similarity, 5 of 41 isolates showed the potential to be introduced as new species. The dominant genera with abounded frequency were found to be Kocuria, Bacillus and Thalassobacillus genera.