Bacterial composition and nutrient removal with a novel PIA-A2/O sewage treatment

2016 ◽  
Vol 73 (11) ◽  
pp. 2722-2730 ◽  
Author(s):  
Li Dong ◽  
Luo Yahong ◽  
Cai Yanan ◽  
Zeng Huiping ◽  
Zhang Jie

A novel post intermittent aeration anaerobic–anoxic–oxic (PIA-A2/O) process was developed to integrate shortcut nitrification–denitrification with denitrifying phosphorus (P) removal for domestic sewage treatment. With the transformation in configuration and phased start-up strategy, the nitritation rate and the ratio of denitrifying phosphorus accumulating organisms to phosphorus accumulating organisms (DPAO/PAO) were enhanced greatly to 88.2% and 83.9–91.7% in the PIA-A2/O process, respectively. Improved total nitrogen and phosphorus removal were achieved at long sludge retention time and low aeration. High sludge activity was maintained through the periodic selection of the additional intermittent aeration zone. High-throughput sequencing revealed that Bacteroidetes (38.96%), Proteobacteria (33.59%), TM7 (4.53%), Chloroflexi (3.09%), and Firmicutes (2.49%) were the dominant phyla in the resultant bacterial community. Abundant anaerobic and facultative bacteria conducive to excess sludge reduction were generated by this process. Potential DPAOs involve the genera of Brevundimonas, Brachymonas, Steroidobacter, Haliscomenobacter, and Rhodocyclus.

2000 ◽  
Vol 42 (3-4) ◽  
pp. 89-94 ◽  
Author(s):  
H.Y. Chang ◽  
C.F. Ouyang

This investigation incorporated a stepwise feeding strategy into the biological process containing anaerobic/oxide/anoxic/oxide (AOAO) stages to enhance nitrogen and phosphorus removal efficiencies. Synthetic wastewater was fed into the experimental reactors during the anaerobic and anoxic stages and the substrates/nutrients were successfully consumed without recycling either nitrified effluent or external carbon source. An intrinsic sufficient carbon source developed during the anoxic stage and caused the NOx (NO2-N+NO3-N) concentration to be reduced from 11.85mg/l to 5.65mg/l. The total Kjeldahl nitrogen (TKN) removal rate was between 81.81%∼93.96% and the PO4-P removal ratio ranged from 93%∼100%. The substrate fed into the anaerobic with a Q1 flow rate and a Q2 into the anoxic reactor. The three difference experiments contained within this study produced Q1/Q2 that varied from 7/3, 8/2, and 9/1. The AOAO process saved nearly one-third of the energy compared with typical biological nutrient removal (BNR) system A2O processes.


2020 ◽  
Vol 81 (9) ◽  
pp. 2023-2032
Author(s):  
Jingqing Gao ◽  
Lei Yang ◽  
Rui Zhong ◽  
Yong Chen ◽  
Jingshen Zhang ◽  
...  

Abstract The environmental problems related to rural domestic sewage treatment are becoming increasingly serious, and society is also concerned about them. A baffled vertical flow constructed wetland (BVFCW) is a good choice for cleaning wastewater. Herein, a drinking-water treatment sludge-BVFCW (D-BVFCW) parallel with ceramsite-BVFCW (C-BVFCW) planted with Oenanthe javanica (O. javanica) to treat rural domestic sewage was investigated, aiming to compare nitrogen and phosphorus removal efficiency in different BVFCWs. A removal of 23.9% NH4+-N, 24.6% total nitrogen (TN) and 76.7% total phosphorus (TP) occurred simultaneously in the D-BVFCW; 56.4% NH4+-N, 60.8% TN and 55.2% TP respectively in the C-BVFCW. The root and plant height increased by an average of 7.9 cm and 8.3 cm, respectively, in the D-BVFCW, and by 0.7 cm and 1.1 cm, respectively, in the C-BVFCW. These results demonstrate that the D-BVFCW and C-BVFCW have different effects on the removal of N and P. The D-BVFCW mainly removed P, while C-BVFCW mainly removed N.


1991 ◽  
Vol 24 (10) ◽  
pp. 231-237
Author(s):  
W. G. Werumeus Buning ◽  
F. W. A. M. Rijnart ◽  
P. P. Weesendorp

To meet two levels of nitrogen and phosphorus removal (effluent standards Ntot 20 and 10 mg/l and Ptot 2 and 1 mg/l respectively) various systems were compared in a desk study. After a cost estimate and an assessment f the advantages and drawbacks, the oxidation ditch with biological by pass phosphate removal turned out to be the best system.


2009 ◽  
Vol 59 (11) ◽  
pp. 2093-2099 ◽  
Author(s):  
H. Lee ◽  
J. Han ◽  
Z. Yun

A lab-scale UCT-type membrane bio-reactor (MBR) was operated for biological nitrogen (N) and phosphorus (P) removal simultaneously. In order to examine biological nutrient removal (BNR) characteristics of MBR, the lab unit was fed with a synthetic strong and weak wastewater. With strong wastewater, a simultaneous removal of N and P was achieved while application of weak wastewater resulted in a decrease of both N and P removal. Recycled nitrate due to the limited organic in weak wastewater operation probably caused a nitrate inhibition in anaerobic zone. In step feed modification with weak wastewater, both N and P removal capability recovered in the system, indicating that the allocation of COD for denitrification at anoxic zone was a key to increase the biological P removal. In addition, the analysis on the specific P uptake rate in anoxic zone demonstrated that denitrifying phosphorus accumulating organism (dPAO) played an important role to remove up to 40% of P along with N. The sludge production characteristics of UCT-type MBR were similar to ordinary activated sludge with BNR capability.


2015 ◽  
Vol 72 (4) ◽  
pp. 528-534 ◽  
Author(s):  
Yang Bai ◽  
Xie Quan ◽  
Yaobin Zhang ◽  
Shuo Chen

A University of Cape Town process coupled with integrated fixed biofilm and activated sludge system was modified by bypass flow strategy (BUCT–IFAS) to enhance nitrogen and phosphorus removal from the wastewater containing insufficient carbon source. This process was operated under different bypass flow ratios (λ were 0, 0.4, 0.5, 0.6 and 0.7, respectively) to investigate the effect of different operational modes on the nitrogen (N) and phosphorus (P) removal efficiency (λ = 0 was noted as common mode, other λ were noted as bypass flow mode), and optimizing the N and P removal efficiency by altering the λ. Results showed that the best total nitrogen (TN) and total phosphorus (TP) removal performances were achieved at λ of 0.6, the effluent TN and TP averaged 14.0 and 0.4 mg/L meeting discharge standard (TN < 15 mg/L, TP < 0.5 mg/L). Correspondingly, the TN and TP removal efficiencies were 70% and 94%, respectively, which were 24 and 41% higher than those at λ of 0. In addition, the denitrification and anoxic P-uptake rates were increased by 23% and 23%, respectively, compared with those at λ of 0. These results demonstrated that the BUCT–IFAS process was an attractive method for enhancing nitrogen and phosphorus removal from wastewater containing insufficient carbon source.


2021 ◽  
Author(s):  
xiaoyan xu ◽  
Jie Jiang ◽  
Zhinan Guo ◽  
Lianglun Sun ◽  
Meizhen Tang

Abstract The mechanism of wastewater treatment based on psychrophilic strains to improve the denitrification efficiency of constructed wetlands at low temperatures has already become a new hotspot. In this study, three mixed psychrophilic strains (Psychrobacter TM-1, Sphingobacterium TM-2 and Pseudomonas TM-3) with high capacity of denitrification were added into a vertical-flow constructed wetlands (CWs), and the effect of the mixed strains on CWs sewage treatment was evaluated. The removal efficiency of chemical oxygen demand (COD), ammonia nitrogen (NH4+-N), total nitrogen (TN) and total phosphorus (TP) was quantified to establish the degradation kinetic model and determine the best dosage of the mixed strains. The effect mechanism of the mixed strains on indigenous microbial community and the change of sewage treatment performance in low temperature constructed wetlands was clarified by high-throughput sequencing technology. The results showed that the mixed strains can effectively remove the organic pollutants (nitrogen and phosphorus) and the optimum dosage of the mixed strain was 2.5%,with average removal rates of 1.52, 2.12, 2.07 and 1.29 times than those of the control. Meanwhile, the dominant strains in the CWs were Proteobacteria (31.23–44.34%), Chloroflexi (12.04–19.05%), Actinobacteria (10.6-20.62%), Acidobacteria (8.23–11.65%), Firmicutes (2.23–15.95%) and Bacteroidetes (4.01–18.9%). These findings provide a basis for the removal of pollutants in constructed wetlands at low temperature.


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