Bioactive Peptides Sensitize Cells to Anticancer Effects of Oxaliplatin in Human Colorectal Cancer Xenografts in Nude Mice

2019 ◽  
Vol 26 (7) ◽  
pp. 512-522
Author(s):  
Xian Li ◽  
Long Xia ◽  
Xiaohui Ouyang ◽  
Qimuge Suyila ◽  
Liya Su ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Colorectal Cancer ◽  
Nude Mice ◽  
Low Dose ◽  
Bioactive Peptides ◽  
Human Colorectal Cancer ◽  
Short Term ◽  
Hct 116

<P>Background: Despite new agent development and short-term benefits in patients with Colorectal Cancer (CRC), metastatic CRC cure rates have not improved due to high rates of oxaliplatin resistance and toxicity. There is an urgent need for effective tools to prevent and treat CRC and reduce morbidity and mortality of CRC patients. Exploring the effects of bioactive peptides on the antitumor to CRC was of vital importance to the clinical application. </P><P> Objective: This study aimed to investigate the therapeutic impact of Anticancer Bioactive Peptides (ACBP) on anticancer effect of oxaliplatin (LOHP) in human colorectal cancer xenografts models in nude mice. </P><P> Methods: HCT-116 cells were cultured in vitro via CCK-8 assays and the absorbance was measured at 450 nm. Apoptosis and cell cycle were assessed by Flow Cytometry (FCM) in vitro. HCT-116 human colorectal cancer cells inoculated subcutaneously in nude mice of treatment with PBS (GG), ACBP, LOHP, ACBP+LOHP (A+L) in vivo. The quality of life was assessed by dietary amount of nude mice, the weight of nude mice, inhibition rates, tumor weight and tumor volume. Immunohistochemistry and RT-qPCR method was conducted to determine the levels of apoptosisregulating proteins/genes in transplanted tumors. </P><P> Results: ACBP induced substantial reductions in viable cell numbers and apoptosis of HCT116 cells in combined with LOHP in vitro. Compared with the control GG group, ACBP combined low dose oxaliplatin (U) group demonstrated significantly different tumor volume, the rate of apoptosis, the expression levels of Cyt-C, caspase-3,8,9 proteins and corresponding RNAs (P<0.05). The expression of pro-apoptotic proteins in the cytoplasm around the nucleus was significantly enhanced by ACBP. Short term intermittent use of ACBP alone indicted a certain inhibitory effect on tumor growth, and improve the quality of life of tumor bearing nude mice. </P><P> Conclusion: ACBP significantly increased the anti-cancer responses of low dose oxaliplatin (L-LOHP), thus, significantly improving the quality of life of tumor-bearing nude mice.</P>

Atractylenolide III induces apoptosis by regulating the Bax/Bcl-2 signaling pathway in human colorectal cancer HCT-116 Cells in vitro and in vivo

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Dan Zhang ◽  
Xiaofang Xiao ◽  
Daqiang Song ◽  
Siwei Chen ◽  
Zhuo Zhang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Signaling Pathway ◽  
Human Colorectal Cancer ◽  
Hct 116 ◽  
Hct 116 Cells ◽  
Atractylenolide Iii

scholarly journals Short-term results of quality of life for curatively treated colorectal cancer patients in Lithuania

Medicina ◽  
2015 ◽  
Vol 51 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Paulius Lizdenis ◽  
Justas Birutis ◽  
Ieva Čelkienė ◽  
Narimantas Samalavičius ◽  
Justas Kuliavas ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Colorectal Cancer ◽  
Cancer Patients ◽  
Short Term ◽  
Colorectal Cancer Patients

scholarly journals Do different doses of acupuncture matter on autonomic nervous activity and symptom management in dysmenorrhea? A randomized controlled trial.

2020 ◽  
Author(s):  
Tsai-Ju Chien ◽  
Yi-Shuo Huang ◽  
Chun-Yu Kuo ◽  
Yu-Ching Cho ◽  
Hsin-Yu Chen ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Low Dose ◽  
Term Effect ◽  
High Dose ◽  
Short Term ◽  
Autonomic Nervous ◽  
Randomized Controlled ◽  
High Control ◽  
Different Doses

Abstract Background The study examined whether the acupuncture dose (number of acupoints stimulated) impacted the efficacy of acupuncture on dysmenorrhea and the relationship with autonomic nervous system regulation.Methods This three-arm randomized controlled study included a high-dose acupuncture (12 acupoints, N = 23), low-dose acupuncture (6 acupoints, N = 30) and control (N = 30) arm. The treatment course was three months. We set heart rate variation (HRV) and analgesics dependence as the primary outcome measurements; Visual Analog Scale (VAS) score, Verbal Multidimensional Scoring System (VMSS) and the 12-Item Short Form Health Survey (SF-12) quality of life questionnaires were set as secondary outcomes. SPSS version 24 was used for data analysis.Results Low-dose acupuncture was superior to high-dose in analgesics dependence (p value: low/high/control: p = 0.043/p = 0.056/p = 0.376); symptom relief (VMSS: low/high/control: p < 0.001/p = 0.007/p = 0.109); and physical quality of life (low/high/control: p < 0.001/p = 0.01/p = 0.007). The groups did not differ in HRV parameters (p > 0.05). In intergroup analysis, more significant changes were noted in the high-dose than in the low-dose group. The scattered nature of the significant changes implies that acupuncture may have a short-term effect on HRV parameters which does not correlate with the acupuncture dose.Conclusions Acupuncture can effectively treat dysmenorrheal pain, improve symptoms and reduce analgesic dependence, but the effect does not correlate with the number of acupoints stimulated. The acupuncture has short-term effect on HRV; yet whether its efficacy on dysmenorrhea is directly related to adjusting the autonomic nervous system may need more large-scale study. It is a safe and effective alternative therapy for dysmenorrhea.Trial: The Efficacy of Different Doses of Acupuncture in Dysmenorrhea, NCT03881319 at ClinicalTrials.gov,

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