scholarly journals OsmiR528 Enhances Cold Stress Tolerance by Repressing Expression of Stress Response-related Transcription Factor Genes in Plant Cells

2019 ◽  
Vol 20 (2) ◽  
pp. 100-114 ◽  
Author(s):  
Wei Tang ◽  
Wells A. Thompson
Keyword(s):  
Transcription Factor ◽  
Stress Response ◽  
Low Temperature ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Molecular Mechanisms ◽  
Plant Cells ◽  
Temperature Tolerance ◽  
Low Temperature Tolerance ◽  
Related Transcription Factor

Background: MicroRNAs participate in many molecular mechanisms and signaling transduction pathways that are associated with plant stress tolerance by repressing expression of their target genes. However, how microRNAs enhance tolerance to low temperature stress in plant cells remains elusive. Objective: In this investigation, we demonstrated that overexpression of the rice microRNA528 (OsmiR528) increases cell viability, growth rate, antioxidants content, ascorbate peroxidase (APOX) activity, and superoxide dismutase (SOD) activity and decreases ion leakage rate and thiobarbituric acid reactive substances (TBARS) under low temperature stress in Arabidopsis (Arabidopsis thaliana), pine (Pinus elliottii), and rice (Oryza sativa). Methods: To investigate the potential mechanism of OsmiR528 in increasing cold stress tolerance, we examined expression of stress-associated MYB transcription factors OsGAMYB-like1, OsMYBS3, OsMYB4, OsMYB3R-2, OsMYB5, OsMYB59, OsMYB30, OsMYB1R, and OsMYB20 in rice cells by qRT-PCR. Results: Our experiments demonstrated that OsmiR528 decreases expression of transcription factor OsMYB30 by targeting a F-box domain containing protein gene (Os06g06050), which is a positive regulator of OsMYB30. In OsmiR528 transgenic rice, reduced OsMYB30 expression results in increased expression of BMY genes OsBMY2, OsBMY6, and OsBMY10. The transcript levels of the OsBMY2, OsBMY6, and OsBMY10 were elevated by OsMYB30 knockdown, but decreased by Os- MYB30 overexpression in OsmiR528 transgenic cell lines, suggesting that OsmiR528 increases low temperature tolerance by modulating expression of stress response-related transcription factor. Conclusion: Our experiments provide novel information in increasing our understanding in molecular mechanisms of microRNAs-associated low temperature tolerance and are valuable in plant molecular breeding from monocotyledonous, dicotyledonous, and gymnosperm plants.

scholarly journals QTL Mapping Low-Temperature Germination Ability in the Maize IBM Syn10 DH Population

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 214
Author(s):  
Qinghui Han ◽  
Qingxiang Zhu ◽  
Yao Shen ◽  
Michael Lee ◽  
Thomas Lübberstedt ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Low Temperature ◽  
Candidate Genes ◽  
Temperature Tolerance ◽  
Bhlh Transcription Factor ◽  
Rna Seq ◽  
Dh Population ◽  
Low Temperature Tolerance ◽  
Germination Ability ◽  
Upregulated Genes

Chilling injury poses a serious threat to seed emergence of spring-sowing maize in China, which has become one of the main climatic limiting factors affecting maize production in China. It is of great significance to mine the key genes controlling low-temperature tolerance during seed germination and study their functions for breeding new maize varieties with strong low-temperature tolerance during germination. In this study, 176 lines of the intermated B73 × Mo17 (IBM) Syn10 doubled haploid (DH) population, which comprised 6618 bin markers, were used for QTL analysis of low-temperature germination ability. The results showed significant differences in germination related traits under optimum-temperature condition (25 °C) and low-temperature condition (10 °C) between two parental lines. In total, 13 QTLs were detected on all chromosomes, except for chromosome 5, 7, 10. Among them, seven QTLs formed five QTL clusters on chromosomes 1, 2, 3, 4, and 9 under the low-temperature condition, which suggested that there may be some genes regulating multiple germination traits at the same time. A total of 39 candidate genes were extracted from five QTL clusters based on the maize GDB under the low-temperature condition. To further screen candidate genes controlling low-temperature germination, RNA-Seq, in which RNA was extracted from the germination seeds of B73 and Mo17 at 10 °C, was conducted, and three B73 upregulated genes and five Mo17 upregulated genes were found by combined analysis of RNA-Seq and QTL located genes. Additionally, the variations of Zm00001d027976 (GLABRA2), Zm00001d007311 (bHLH transcription factor), and Zm00001d053703 (bZIP transcription factor) were found by comparison of amino sequence between B73 and Mo17. This study will provide a theoretical basis for marker-assisted breeding and lay a foundation for further revealing molecular mechanism of low-temperature germination tolerance in maize.

Morphological and cytological characters associated with low-temperature tolerance in wheat (Triticum aestivum L. em Thell.)

2000 ◽  
Vol 80 (4) ◽  
pp. 687-692 ◽  
Author(s):  
A. E. Limin ◽  
D. B. Fowler
Keyword(s):  
Triticum Aestivum ◽  
Low Temperature ◽  
Stress Tolerance ◽  
Cell Size ◽  
Plant Morphology ◽  
Triticum Aestivum L ◽  
Temperature Tolerance ◽  
Leaf Length ◽  
Low Temperature Tolerance ◽  
Highly Correlated

Attempts to associate morphological or cytological characters with low-temperature (LT) tolerance in wheat (Triticum aestivum L. em. Thell.) and other members of the Triticeae group have met with ambiguous or contradictory results. Consequently no single character has emerged that can be considered a reliable predictor of LT tolerance. Twenty-six winter wheat cultivars of diverse origin were analyzed to determine the association among leaf length, width, area and cell size (guard cell length) and their association with LT stress tolerance. Measurements were made on plants grown at 4 °C and at 17 °C to determine if expression of LT tolerance associated characters was temperature dependent. At 4 °C, all individual leaf characters measured, including cell size, were very highly correlated with LT tolerance and with each other. Undisturbed plant height was not significantly correlated with LT tolerance until 5 wk of growth at 4 °C and reached its highest correlation at 10 wk when the plants were on average at their most prostrate state of growth. Growth at 17 °C resulted in much weaker relationships among all characters. At 4 °C short narrow leaves and small cell size were the best indicators of LT stress tolerance. Prostrate growth habit of plants grown at LT was also a good indicator of plant LT tolerance, but measurements of this character did not improve prediction equations based on leaf characters and cell size. Key words: Low-temperature tolerance, plant morphology, cell size, leaf characteristics, Triticum aestivum

AP2/ERF, an important cold stress-related transcription factor family in plants: A review

2021 ◽  
Vol 27 (9) ◽  
pp. 1953-1968
Author(s):  
Faujiah Nurhasanah Ritonga ◽  
Jacob Njaramba Ngatia ◽  
Yiran Wang ◽  
Muneer Ahmed Khoso ◽  
Umar Farooq ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cold Stress ◽  
Transcription Factor Family ◽  
Related Transcription Factor
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