MicroRNA156 amplifies transcription factor-associated cold stress tolerance in plant cells

2018 ◽  
Vol 294 (2) ◽  
pp. 379-393 ◽  
Author(s):  
Mingqin Zhou ◽  
Wei Tang
Keyword(s):  
Transcription Factor ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Plant Cells

scholarly journals OsmiR528 Enhances Cold Stress Tolerance by Repressing Expression of Stress Response-related Transcription Factor Genes in Plant Cells

2019 ◽  
Vol 20 (2) ◽  
pp. 100-114 ◽  
Author(s):  
Wei Tang ◽  
Wells A. Thompson
Keyword(s):  
Transcription Factor ◽  
Stress Response ◽  
Low Temperature ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Molecular Mechanisms ◽  
Plant Cells ◽  
Temperature Tolerance ◽  
Low Temperature Tolerance ◽  
Related Transcription Factor

Background: MicroRNAs participate in many molecular mechanisms and signaling transduction pathways that are associated with plant stress tolerance by repressing expression of their target genes. However, how microRNAs enhance tolerance to low temperature stress in plant cells remains elusive. Objective: In this investigation, we demonstrated that overexpression of the rice microRNA528 (OsmiR528) increases cell viability, growth rate, antioxidants content, ascorbate peroxidase (APOX) activity, and superoxide dismutase (SOD) activity and decreases ion leakage rate and thiobarbituric acid reactive substances (TBARS) under low temperature stress in Arabidopsis (Arabidopsis thaliana), pine (Pinus elliottii), and rice (Oryza sativa). Methods: To investigate the potential mechanism of OsmiR528 in increasing cold stress tolerance, we examined expression of stress-associated MYB transcription factors OsGAMYB-like1, OsMYBS3, OsMYB4, OsMYB3R-2, OsMYB5, OsMYB59, OsMYB30, OsMYB1R, and OsMYB20 in rice cells by qRT-PCR. Results: Our experiments demonstrated that OsmiR528 decreases expression of transcription factor OsMYB30 by targeting a F-box domain containing protein gene (Os06g06050), which is a positive regulator of OsMYB30. In OsmiR528 transgenic rice, reduced OsMYB30 expression results in increased expression of BMY genes OsBMY2, OsBMY6, and OsBMY10. The transcript levels of the OsBMY2, OsBMY6, and OsBMY10 were elevated by OsMYB30 knockdown, but decreased by Os- MYB30 overexpression in OsmiR528 transgenic cell lines, suggesting that OsmiR528 increases low temperature tolerance by modulating expression of stress response-related transcription factor. Conclusion: Our experiments provide novel information in increasing our understanding in molecular mechanisms of microRNAs-associated low temperature tolerance and are valuable in plant molecular breeding from monocotyledonous, dicotyledonous, and gymnosperm plants.

scholarly journals Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Lei Huang ◽  
Yongbo Hong ◽  
Huijuan Zhang ◽  
Dayong Li ◽  
Fengming Song
Keyword(s):  
Transcription Factor ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Nac Transcription Factor

scholarly journals ERF Transcription Factor OsBIERF3 Positively Contributes to Immunity against Fungal and Bacterial Diseases but Negatively Regulates Cold Tolerance in Rice

2022 ◽  
Vol 23 (2) ◽  
pp. 606
Author(s):  
Yongbo Hong ◽  
Hui Wang ◽  
Yizhou Gao ◽  
Yan Bi ◽  
Xiaohui Xiong ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Abiotic Stress ◽  
Carboxylic Acid ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Cold Tolerance ◽  
Transcriptional Activation ◽  
Altered Expression ◽  
Erf Transcription Factor

We previously showed that overexpression of the rice ERF transcription factor gene OsBIERF3 in tobacco increased resistance against different pathogens. Here, we report the function of OsBIERF3 in rice immunity and abiotic stress tolerance. Expression of OsBIERF3 was induced by Xanthomonas oryzae pv. oryzae, hormones (e.g., salicylic acid, methyl jasmonate, 1-aminocyclopropane-1-carboxylic acid, and abscisic acid), and abiotic stress (e.g., drought, salt and cold stress). OsBIERF3 has transcriptional activation activity that depends on its C-terminal region. The OsBIERF3-overexpressing (OsBIERF3-OE) plants exhibited increased resistance while OsBIERF3-suppressed (OsBIERF3-Ri) plants displayed decreased resistance to Magnaporthe oryzae and X. oryzae pv. oryzae. A set of genes including those for PRs and MAPK kinases were up-regulated in OsBIERF3-OE plants. Cell wall biosynthetic enzyme genes were up-regulated in OsBIERF3-OE plants but down-regulated in OsBIERF3-Ri plants; accordingly, cell walls became thicker in OsBIERF3-OE plants but thinner in OsBIERF3-Ri plants than WT plants. The OsBIERF3-OE plants attenuated while OsBIERF3-Ri plants enhanced cold tolerance, accompanied by altered expression of cold-responsive genes and proline accumulation. Exogenous abscisic acid and 1-aminocyclopropane-1-carboxylic acid, a precursor of ethylene biosynthesis, restored the attenuated cold tolerance in OsBIERF3-OE plants while exogenous AgNO3, an inhibitor of ethylene action, significantly suppressed the enhanced cold tolerance in OsBIERF3-Ri plants. These data demonstrate that OsBIERF3 positively contributes to immunity against M. oryzae and X. oryzae pv. oryzae but negatively regulates cold stress tolerance in rice.

The NAC transcription factor CaNAC064 is a regulator of cold stress tolerance in peppers

Plant Science ◽  
2020 ◽  
Vol 291 ◽  
pp. 110346 ◽  
Author(s):  
Xiao-ming Hou ◽  
Hua-feng Zhang ◽  
Su-ya Liu ◽  
Xin-ke Wang ◽  
Yu-meng Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Nac Transcription Factor

scholarly journals AtCIPK19 aggrandizes polyamines-involved cold stress tolerance in plant cells

2019 ◽  
pp. 03-21
Author(s):  
Wei Tang ◽  
Wells Thompson
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Molecular Breeding ◽  
Ion Homeostasis ◽  
Plant Cells ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Interacting Protein ◽  
Calcineurin B

The CALCINEURIN B-LIKE PROTEIN-INTERACTING PROTEIN KINASEs (CIPKs) play important roles in regulating ion homeostasis and stress responses. However, molecular mechanism of the Arabidopsis (Arabidopsis thaliana) CALCINEURIN B-LIKE PROTEIN-INTERACTING PROTEIN KINASE19 (CIPK19) enhanced cold stress tolerance is not fully understood. Here, we report that overexpression of the Arabidopsis CIPK19 gene (AtCIPK19) results in increasing cell viability and growth rate under cold stress in O. sativa, G. hirsutum, and P. strobus. AtCIPK19 increases cold stress tolerance by decreases the thiobarbituric acid reactive substances (TBARS) and increases the levels of putrescine (Put), spermidine (Spd), and spermine (Spm). In AtCIPK19 transgenic rice cell lines, the transcript levels of genes associated with biosynthesis of Put, Spd, and Spm include OsADC1, OsADC2, OsADC3, OsODC1, OsODC2, OsODC3, OsCPA1, OsCPA2, OsCPA3, OsAIH, OsSAMDC1, OsSAMDC2, OsSAMDC3, OsSAMDC4, OsSAMDC5, OsSAMDC6, OsSPD/SPM1, OsSPD/SPM2, OsSPD/SPM3, and OsSPD/SPM4 are increased under cold stress. These results will increase our understanding of AtCIPK19-related cold stress tolerance in different plant species and are valuable in plant molecular breeding application. Keywords: CALCINEURIN B-LIKE PROTEIN; Cold stress; Pinus; Putrescine; Spermidine; Spermine

Characterization of a transcription factor SlNAC7 gene from Suaeda liaotungensis and its role in stress tolerance

2021 ◽  
Author(s):  
Hong-Fei Wang ◽  
Hong-Yan Shan ◽  
He Shi ◽  
Dan-Dan Wu ◽  
Tong-Tong Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Tolerance

scholarly journals Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance

2020 ◽  
Vol 21 (6) ◽  
pp. 2177 ◽  
Author(s):  
Bo Li ◽  
Jia-Cheng Zheng ◽  
Ting-Ting Wang ◽  
Dong-Hong Min ◽  
Wen-Liang Wei ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Hairy Roots ◽  
Yeast Cells ◽  
Salt Stress Tolerance ◽  
Drought And Salt Stress

Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes (GmVOZs) have been reported to exist in the soybean (Glycine max) genome. In spite of this, little information is currently available regarding GmVOZs. In this study, GmVOZs were cloned and characterized. GmVOZ genes encode proteins possessing transcriptional activation activity in yeast cells. GmVOZ1E, GmVOZ2B, and GmVOZ2D gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. GmVOZs displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, GmVOZ1G showed a significantly induced expression in response to all stress treatments. Overexpression of GmVOZ1G in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing GmVOZ1G were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, GmVOZ1G positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress.

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