Preparation of β-CD–Quercetin Complex and its Effects on Ethanol- Damaged BRL-3A Hepatocytes

2020 ◽  
Vol 17 (8) ◽  
pp. 720-726
Author(s):  
Yingxia Zhang ◽  
Xiao Lin ◽  
Jinglong Wang ◽  
Sun Jing ◽  
Deya Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sustained Release ◽  
Gel Electrophoresis ◽  
Tail Moment ◽  
Protection Mechanism ◽  
Rate Analysis ◽  
Single Cell Gel Electrophoresis ◽  
Hematoxylin Eosin ◽  
Scanning Electron

Objective: To prepare the sustained-release complex, quercetin was incorporated with β- cyclodextrin (β-CD) and the effect of β-CD–quercetin complex on the growth of ethanol-injuried hepatocytes was studied. Methods: By using scanning electron microscopy, infrared spectroscopy, and release rate analysis, β- CD–quercetin complex was identified. The effect of different concentrations of β-CD–quercetin complex on the growth of ethanol-damaged hepatocytes at different time was observed by using MTT assay, and the cell quantity and morphology were observed by using hematoxylin–eosin staining. By using single-cell gel electrophoresis, the prevention of β-CD–quercetin complex from the DNA damage of ethanol-damaged BRL-3A cells was studied, and Olive tail moment was calculated. Results: β-CD–quercetin complex as the sustained-release complex was successfully prepared. The ethanol induced damage of BRL-3A cells could be prevented by 20, 40 and 80 mg/L of quercetin complex, and the protection mechanism of hepatocyte was related to the antioxidation of DNA. Conclusion: Quercetin sustained-release complex could be prepared with β-CD, and it might be used to treat alcoholic liver disease.

Morphological Characterization of a Sustained-Release Drug Implant by Scanning Electron Microscopy, Polarized Light Microscopy and Image Analysis

1998 ◽  
Vol 4 (S2) ◽  
pp. 932-933
Author(s):  
J.P. Neilly ◽  
J.S. Deng ◽  
J.L. House ◽  
J.A. Fagerland
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sustained Release ◽  
Light Microscopy ◽  
Polymer Matrix ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Sustained Drug Release ◽  
Gentamicin Sulfate ◽  
Scanning Electron

Septacin is a sustained-release antibiotic currently under development by the Hospital Products Division of Abbott Laboratories. The product is designed to be used as an anti-infective implant in orthopedic surgical procedures with a sustained drug release for up to six weeks in vivo. It consists of gentamicin sulfate formulated with a bioerodable polyanhydride copolymer. The polymer is biodegradable and has been approved by the FDA for human clinical trials. The final product is obtained by mixing 20% gentamicin sulfate with molten polymer and injection molding it to form cylindrical Septacin beads.The microstructure of drug particles and polymer matrix is critical to the performance of sustained release products, thus scanning electron microscopy (SEM) and polarized light microscopy (PLM) were utilized in this study. SEM has proven useful for evaluating the microstructure of drug formulations3 and was used to examine the drug-polymer matrix structure. Average drug particle size and distribution were determined, and the drug-polymer boundary was evaluated.

scholarly journals Structure and microstructure of coronary dentin in non-erupted human deciduous incisor teeth

2002 ◽  
Vol 13 (3) ◽  
pp. 170-174 ◽  
Author(s):  
Luciane R.R S. Costa ◽  
Ii-Sei Watanabe ◽  
Márcia C. Kronka ◽  
Marcelo C.P. Silva
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nitric Acid ◽  
Light Microscopy ◽  
Three Dimensional ◽  
Dentinal Tubules ◽  
Sem Study ◽  
Structure And Microstructure ◽  
Hematoxylin Eosin ◽  
Scanning Electron

The dentin structure of non-erupted human deciduous mandibular and maxillary central and lateral incisor teeth was studied employing light and scanning electron microscopy. For light microscopy, nitric-acid-demineralized and ground sections were used. The sections were stained by hematoxylin-eosin, picrosirius and azo-carmim methods, and ground specimens were prepared using a carborundum disk mounted in a handpiece. For SEM study, teeth were frozen in liquid nitrogen and fractured at longitudinal and transversal directions. Structurally, demineralization and ground methods revealed tubules with primary and secondary curvatures, canaliculi, giant tubules, interglobular dentin, predentin, and intertubular dentin. Scanning electron microscopy showed three-dimensional aspects of dentinal tubules, canaliculi, peritubular dentin, intertubular dentin, and predentin. This study contributes to knowledge about dentin morphology showing characteristics of teeth not yet submitted to mastication stress.

Scanning Electron Microscopy and Gel Electrophoresis of Vascularized Periosteal Autografts

1989 ◽  
Vol 83 (3) ◽  
pp. 500-510 ◽  
Author(s):  
Fernando D. Burstein ◽  
Rinaldo F. Canalis ◽  
Ernesto M. Canalis ◽  
Stephan Ariyan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Gel Electrophoresis ◽  
Scanning Electron

scholarly journals Two new species of the family Nippobodidae (Acari, Oribatida), including a description of the leg-folding process

ZooKeys ◽  
2018 ◽  
Vol 781 ◽  
pp. 109-139
Author(s):  
Nestor Fernandez ◽  
Pieter Theron ◽  
Sergio Leiva
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Genital Plate ◽  
Anal Plate ◽  
Protection Mechanism ◽  
Folding Process ◽  
The Family ◽  
Two New Species ◽  
Humeral Condyle ◽  
Scanning Electron

Nippobodespanemorfissp. n.andLeobodestrypasissp. n.are described by means of optical and Scanning Electron Microscopy (SEM) and compared to other congeners. The leg-folding process is described and illustrated.Nippobodespanemorfissp. n.is characterised by interlocking, double hook-shaped, posterior prodorsal condyle and anterior zone humeral apophysis; posterior prodorsal depression present. Tutorium a large lamina defining a pocket-shaped structure; bothridial opening ovoid, situated at the bottom of a U-shaped structure; deep, rounded-ovoid anterior notogastral depression present; ten pairs of notogastral setae;csetae looped, dentate, sharply tipped. Marginal setaeh3,p3on large promontories, followed by deep V-shaped incision; notogaster completely surrounded by circumgastric depression; lateral genital zone with locking structure constituted by longitudinal cuticular elevation, with promontories and a parallel furrow involved in the leg-folding process; genital plate smaller than anal plate.Leobodestrypasissp. n.is characterised by: the presence of posterior prodorsal depression and anterior notogastral depression; bridge-shaped anterior prodorsal condyles; heart-shaped frontal prodorsal orifice; ten pairs of notogastral setae; posterior prodorsal condyle and humeral condyle interlocked, forming double hook-like structure; circumgastric furrow surrounding entire notogaster; setaelp, h2, h1situated on shallow medial furrow; notogastral setaelm,lp,h1,h2medially aligned;p1, p2, p3, h3marginally situated. Legs I-IV, tutorium, pedotectum I, and pedotectum II involved in leg folding which is inferred to be a protection mechanism.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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