Antiplasmodial and cytotoxic activities of two Euphorbiaceae family plants, Euphorbia hirta and Euphorbia thymifolia

2021 ◽  
Vol 17 ◽  
Author(s):  
Jigna Vadalia ◽  
Jalpa Sanandia ◽  
Vaibhav Bhatt ◽  
Navin Sheth
Keyword(s):  
Cell Line ◽  
Mtt Assay ◽  
Methanol Extract ◽  
Carcinoma Cell ◽  
Huh7 Cell ◽  
Carcinoma Cell Line ◽  
Liver Carcinoma ◽  
Cytotoxic Activities ◽  
Euphorbia Hirta

Background: Malaria is a major disease of developing countries and the main issue in its control of resistance against all available antimalarial drugs. Our aim is to find affordable therapy for this worldwide problem. The present study was conducted to evaluate the antiplasmodial and cytotoxic activities of Euphorbia hirta and Euphorbia thymifolia. Methods: The antiplasmodial activity was evaluated against chloroquine-sensitive (MRC-2) and chloroquine-resistant (RKL-9) strain of Plasmodium falciparum by schizont maturation inhibition and Plasmodium lactate dehydrogenase (PfLDH) activity assays. The cytotoxicity was performed by MTT assay on Huh7 liver carcinoma cell line. Results: IC50 for PfLDH activity of E. hirta and E. thymifolia methanol extract against P. faciparum MRC-2 strain was 9.6 and 12.6 µg/mL, respectively and against P. faciparum RKL-9 strain was 10.2 and 11.56 µg/mL, respectively. Methanol extract of E. thymifolia showed lower IC50 (19.3 µg/mL) than methanol extract of E. hirta (24.7 µg/mL) in MTT assay against Huh7 cell line. Conclusion: Both the plant methanol extracts showed a two-fold lower cytotoxic activity against hepatocyte derived carcinoma cell line (Huh7) compared to in vitro inhibitory activity against P. falciparum strains (MRC-2 and RKL-9).

scholarly journals Antisense EGFR sequence reverses the growth properties of human liver carcinoma cell line BEL-7404 in vitro

Cell Research ◽  
1993 ◽  
Vol 3 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Yonghua Xu ◽  
Wanli Jiang ◽  
Sufeng Peng ◽  
Yinghua Chen
Keyword(s):  
Cell Line ◽  
Human Liver ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Liver Carcinoma ◽  
Growth Properties

scholarly journals Does Bromelain-Cisplatin Combination Afford In-Vitro Synergistic Anticancer Effects on Human Prostatic Carcinoma Cell Line, PC3?

2020 ◽  
Vol 9 ◽  
pp. 1749
Author(s):  
Fatemeh Amini Chermahini ◽  
Elham Raeisi ◽  
Mathias Hossain Aazami ◽  
Abbas Mirzaei ◽  
Esfandiar Heidarian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Viability ◽  
Cell Line ◽  
Colony Formation ◽  
Prostatic Carcinoma ◽  
Carcinoma Cell ◽  
Quantitative Polymerase Chain Reaction ◽  
Chemotherapeutic Agents ◽  
Carcinoma Cell Line

Background: Bromelain enhances anticancer impacts to chemotherapeutic agents. The question as to whether bromelain does promote in-vitro cytotoxic and proapoptotic effects of cisplatin on human prostatic carcinoma PC3 cell line was investigated. Materials and Methods: PC3 (human prostatic carcinoma) cells were treated either single or in combination with bromelain and/or cisplatin. MTT, clonogenic assay, flow cytometry and real-time quantitative polymerase chain reaction were used to investigate cell viability, colony formation, proapoptotic potential and p53 gene expression, respectively. Results: Cisplatin (IC10) combined with bromelain (IC40) significantly affected PC3 cell viability, inhibited colony formation, as well increased p53 proapoptotic gene expression compared to cisplatin single treatment. Nevertheless, bromelain-cisplatin chemoherbal combination did not display any additive proapoptotic effect compared to single treatments. Conclusion: Bromelain-cisplatin chemoherbal combination demonstrated synergistic in-vitro anticancer effect on human prostatic carcinoma cell line, PC3, that drastically reduced required cisplatin dose. [GMJ.2020;9:e1749]

scholarly journals Novel Synthesis Method of Micronized Ti-Zeolite Na-A and Cytotoxic Activity of Its Silver Exchanged Form

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
H. F. Youssef ◽  
W. H. Hegazy ◽  
H. H. Abo-almaged ◽  
G. T. El-Bassyouni
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Synthesis Method ◽  
Carcinoma Cell Line ◽  
Breast Adenocarcinoma ◽  
Synthetic Process ◽  
Colon Cell ◽  
Colon Cell Line

The core-shell method is used as a novel synthetic process of micronized Ti-Zeolite Na-A which involves calcination at 700°C of coated Egyptian Kaolin with titanium tetrachloride in acidic medium as the first step. The produced Ti-coated metakaolinite is subjected to microwave irradiation at low temperature of 80°C for 2 h. The prepared micronized Ti-containing Zeolites-A (Ti-Z-A) is characterized by FTIR, XRF, XRD, SEM, and EDS elemental analysis. Ag-exchanged form of Ti-Z-Ag is also prepared and characterized. The Wt% of silver exchanged onto the Ti-Zeolite structure was determined by atomic absorption spectra. Thein vitrocytotoxic activity of Ti-Z-Ag against human hepatocellular carcinoma cell line (HePG2), colon cell line carcinoma (HCT116), lung carcinoma cell line (A549), and human Caucasian breast adenocarcinoma (MCF7) is reported. The results were promising and revealed that the exchanged Ag form of micronized Ti-Zeolite-A can be used as novel antitumor drug.

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