Isolation and Purification of Kudinosides from Kuding Tea by Semi-Preparative HPLC Combined with MCI-GEL Resin

2020 ◽  
Vol 16 (7) ◽  
pp. 914-923
Author(s):  
Ji Tian ◽  
Xuanyuan Wang ◽  
Qingxin Shi ◽  
Xingliang Xiang ◽  
Chao Su ◽  
...  

Background: Kuding tea, a Traditional Chinese drink, has a history of thousands of years in China. Triterpenoid saponins in Kuding tea are regarded as one of the major functional ingredients. Objective: The aim of this paper was to establish separation progress for the isolation and purification of five triterpenoid saponins (kudinoside A, C, D, F, G) from Kuding tea. Methods: Nine types of resins, including seven macroporous resins and two MCI-GEL resins, were firstly used for purifying triterpenoid saponins by the adsorption and desorption tests. Further dynamic adsorption/desorption experiments were carried out to obtain the optimal parameters for the five targeted saponins. Then the purification of five triterpenoid saponins (kudinoside A, C, D, F, G) was completed by semi-preparative high-performance liquid chromatography (semi-pHPLC). Results: As of optimized results, the HP20SS MCI-GEL was selected as the optimal one. The data also showed that 65.24 mg of refined extract including 7.04 mg kudinoside A, 3.52 mg kudinoside C, 4.04 mg kudinoside D, 4.13 mg kudinoside F, and 34.45 mg kudinoside G, could be isolated and purified from 645.90 mg of crude extract in which the content of five saponins was 81.51% and the average recovery reached 69.76%. The final contents of five saponins increased 6.91-fold as compared to the crude extract. Conclusion: The established separation progress was highly efficient, making it a potential approach for the large-scale production in the laboratory and providing several markers of triterpenoid saponins for quality control of Kuding tea or its processing products.

Author(s):  
Bekhruzi Talbi Shokhzoda ◽  
Mikhail Georgievich Tyagunov

Looking at the history of solar energy and renewable energy in general, the authorities and scientists have been paying much attention to the recent period, due to the depletion of fossil energy resources and the growing difficulties in solving environmental problems. The development of solar energy has led to the use of solar energy concentrators. Concentrators are used to concentrate sunlight onto PV cells. This allows for a reduction in the cell area required for producing a given amount of power. The goal is to significantly reduce the cost of electricity generated by replacing expensive PV converter area with less expensive optical material. In this chapter, the authors talk about concentrators in solar energy, especially about modules based on holographic films. Holographic solar panels (HSP) in recent decades have appeared in large-scale production and been actively used in solar energy. Evaluations of other types of existing concentrators are presented.


RSC Advances ◽  
2015 ◽  
Vol 5 (47) ◽  
pp. 37830-37836 ◽  
Author(s):  
Wei Wei ◽  
Linlin Guo ◽  
Xiaoyang Qiu ◽  
Peng Qu ◽  
Maotian Xu ◽  
...  

Although many routes have been developed that can efficiently improve the electrochemical performance of LiFePO4 cathodes, few of them meet the urgent industrial requirements of large-scale production, low cost and excellent performance.


Nanoscale ◽  
2021 ◽  
Author(s):  
Adrián Romaní Vázquez ◽  
Christof Neumann ◽  
Mino Borrelli ◽  
Huanhuan Shi ◽  
Matthias Kluge ◽  
...  

Graphene and related materials have been widely studied for their superior properties in a wide field of applications. However, large-scale production remains a critical challenge to enable commercial acceptance. Here,...


2018 ◽  
Vol 1 (12) ◽  
pp. 6986-6991 ◽  
Author(s):  
Xing Gao ◽  
Ying Du ◽  
Junwen Zhou ◽  
Siwu Li ◽  
Pengfei Qi ◽  
...  

Separations ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 73
Author(s):  
Michaela Nováková ◽  
Tereza Fábryová ◽  
Doris Vokurková ◽  
Iva Dolečková ◽  
Jiří Kopecký ◽  
...  

Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium Synechocystis salina (S. salina) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of n-heptane–ethanol–water (2:4:4, v/v/v) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health.


2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Maria Carinnes P. Alejandria ◽  
Tisha Isabelle M. De Vergara ◽  
Karla Patricia M. Colmenar

AbstractThe practice of making and eating fertilized duck eggs is a widely known practice in Asia. In the Philippines, “balut” is a popularly known Filipino delicacy which is made by incubating duck eggs for about 18 days. However, criticisms against its authenticity and the unstable demand for balut in the market pose challenges to the development of the Philippine balut industry. Consequently, this research aims to trace the history of balut production and consumption in the Philippines by specifically looking into the following. First, it explores the factors that contribute to the discovery and patronage of balut. Second, it identifies the localities that popularized the balut industry. Third, this includes the key industries that started the large scale production of balut. Fourth, it discusses the local ways of balut-making practices in the country. Lastly, it also provides an account of the ways of balut consumption. Through content analysis of secondary data, this research argues that balut remains an authentic Filipino food despite shared patronage in several Asian countries through the localized meanings associated with its consumption, preparation, and distribution.


ChemSusChem ◽  
2013 ◽  
Vol 6 (6) ◽  
pp. 1084-1090 ◽  
Author(s):  
Haitao Zhang ◽  
Xiong Zhang ◽  
Xianzhong Sun ◽  
Dacheng Zhang ◽  
He Lin ◽  
...  

2019 ◽  
Vol 7 (40) ◽  
pp. 22958-22966 ◽  
Author(s):  
Shenghui Shen ◽  
Shengzhao Zhang ◽  
Shengjue Deng ◽  
Guoxiang Pan ◽  
Yadong Wang ◽  
...  

Herein, we firstly proposed multidimensional titanium niobium oxides (1D/2D/3D-TNO) via a versatile bioinspired template method, which employed as high-performance anodes for both liquid and solid state lithium ion batteries


Sign in / Sign up

Export Citation Format

Share Document