Synthesis and characterisation of super-paramagnetic iron oxide nanoparticles (SPIONs) for minimising Aeromonas hydrophila load from freshwater

2021 ◽  
Vol 17 ◽  
Author(s):  
Munish Kumar ◽  
Gyandeep Gupta ◽  
Tincy Varghese ◽  
Aruna M Shankregowda ◽  
Prem Prakash Srivastava ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Exposure Time ◽  
Aeromonas Hydrophila ◽  
Antibacterial Effect ◽  
Bacterial Load ◽  
Effective Concentration ◽  
Precipitation Method ◽  
Iron Oxide Nanoparticle ◽  
Super Paramagnetic Iron Oxide ◽  
High Bacterial Load

Background: The current study was conducted to prepare an efficient super-paramagnetic iron oxide nanoparticle (SPIONs) to remove Aeromonas hydrophila from water. Methods: The nanoparticles were synthesized by the co-precipitation method and characterized by DLS, UV-Vis spectrophotometer, FT-IR, XRD, FEG-TEM, and VSM analysis. Results and Discussion: The results showed that the synthesized SPIONs were having a size range of 8-12nm with magnetic property. Bacteria removal efficiency and antibacterial activity of SPIONs were assessed in sterile distilled water by adding different concentrations of SPIONs viz. 0, 6.25, 12.5, 25, 50, 100, 200, 500, and 1000µM with different initial bacterial loads viz. 1×103, 1×104, 1×105, 1×106, and 1×107 CFU mL−1 at different time intervals 15, 30, 45, and 60 min. At low bacterial load (1×103 to 1×105 CFU mL−1), 95 to 99.99% of bacteria were removed by low SPIONs concentration (6.25-100µM) by 15min which was increased up to 100% by 30min. However, at high bacterial load (1×106 to 1×107 CFU mL−1), more than 87 to 95% of bacteria were removed by the highest SPIONs concentration (1000µM) by 15min, which was increased above 93 to 99.99% by increasing the exposure time to 60min. At low bacterial load (1×103 to1×105 CFU mL−1), the effective concentration was 3.21 to 6.42µM at 15-60 min intervals. Meanwhile, the effective concentration at high bacterial load was 267.81 µM at 15min, which was decreased to 104.09 µM with increasing exposure time to 60min. Conclusion: Based on the results, it is concluded that the antibacterial effect against A. hydrophila depends on the concentration as well as the exposure time of SPIONs. A low concentration of SPIONs is sufficient to remove 100% of bacterial load in lower exposure time and increasing concentration of SPIONs increases the antibacterial effect. However, further research requires to find the safe concentration of SPIONs for using it as a novel antibacterial agent for the treatment of aeromonads disease in aquaculture.

Epirubicin loaded super paramagnetic iron oxide nanoparticle-aptamer bioconjugate for combined colon cancer therapy and imaging in vivo

2013 ◽  
Vol 50 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Seyed Hamid Jalalian ◽  
Seyed Mohammad Taghdisi ◽  
Nasim Shahidi Hamedani ◽  
Seyedeh Alia Moosavian Kalat ◽  
Parirokh Lavaee ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Iron Oxide ◽  
Cancer Therapy ◽  
Iron Oxide Nanoparticle ◽  
Super Paramagnetic Iron Oxide ◽  
Oxide Nanoparticle

scholarly journals Antiferromagnetic switch in serum

2017 ◽  
Author(s):  
Sufi O. Raja ◽  
Sanjay Chatterjee ◽  
Anjan Kr. Dasgupta
Keyword(s):  
Magnetic Field ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Iron Homeostasis ◽  
Ferritin Level ◽  
Correlation Spectroscopy ◽  
Iron Oxide Nanoparticle ◽  
Oxide Nanoparticles ◽  
Iron Storage ◽  
Super Paramagnetic Iron Oxide

1.AbstractFerritin contains naturally occurring iron oxide nanoparticle surrounded by a structured spherical array of peptide residues that provides tremendous stability to this iron storage protein. We use synthetic citrate coated Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) and static magnetic field in exploring the Ferritin induced magnetic environment of human serum samples with varying ferritin level collected from thalassemic patients. We report anti-ferromagnetic properties of serum in patients with iron overloading. Magnetic pulling by an external magnetic field showed a cusp-like behavior with increasing concentration of serum Ferritin measured by standard ELISA based kit. A reduction in the extent of pulling after a threshold concentration of Ferritin (1500 ng/ml) suggests a Ferritin dependent magnetic switching.Negative magnetization (anti-ferromagnetization) was confirmed by Vibrating Sample Magnetometric (VSM) analysis of SPION-serum mixture containing very high level of Ferritin. Such magnetic switching may have a possible role in iron homeostasis during overloading of Ferritin.3.AbbreviationsSPIONs: Super Paramagnetic Iron Oxide Nanoparticles, VSM: Vibrating Sample Magnetometry, SQUID: Super conducting Quantum Interference Device, PCS: Photon Correlation Spectroscopy

Investigation of Magnetic Iron Oxide Nanoparticle Properties with Co-Precipitation Methods under Different Reaction Conditions

2020 ◽  
Vol 10 ◽  
Author(s):  
Sema Salgın ◽  
Uğur Salgın ◽  
Nagihan Soyer
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Morphological Structure ◽  
Precipitation Method ◽  
Iron Oxide Nanoparticle ◽  
Hydrodynamic Diameter ◽  
Oxide Nanoparticles ◽  
Magnetic Iron Oxide Nanoparticles ◽  
Magnetic Iron Oxide ◽  
Co Precipitation

: In this study, magnetic iron oxide nanoparticles were synthesized by cost effective and an easy co-precipitation method. The effects of reaction parameters such as the mole ratio of Fe2+/Fe3+ (1/1, 1/2 and 1/3) and the base type (NaOH and NH4OH) on the average hydrodynamic diameter, zeta potential, chemical and morphological structure and saturation magnetization values of magnetic iron oxide nanoparticles were systematically investigated. Magnetic iron oxide nanoparticles synthesized at the mole ratio of 1/2 using NH4OH base gave better results in terms of particle size and particle shape, magnetization value. In order to prevent the formation of different phases in the co-precipitation method, an alternative approach was developed and the reaction was performed using inert supercritical CO2 atmosphere.

scholarly journals A Comparative Study of Iron Oxide Nanoparticles Surface Modified Using Carboxylic Acids

2021 ◽  
Vol 8 (1) ◽  
pp. 116-125
Author(s):  
Archana S.
Keyword(s):  
Surface Modification ◽  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Comparative Study ◽  
Succinic Acid ◽  
Iron Oxide Nanoparticles ◽  
The Body ◽  
Precipitation Method ◽  
Iron Oxide Nanoparticle ◽  
Oxide Nanoparticles

In the last decade nanotechnology has greatly developed in many research fields such as engineering, electronic, biological and many others. They can offer several possibilities to design tools, to create new techniques or improve the already existing ones, to discover innovative applications. Nano-science is one of the most important research and development frontiers in modern science. Nanotechnology is now widely used throughout the pharmaceutical industry, medicine, electronics, robotics, and tissue engineering. For biological and biomedical applications, magnetic iron oxide nanoparticles are the primary choice because of their biocompatibility, super-paramagnetic behavior and chemical stability. The purpose of this work is the design, development and surface modification of magnetic nanoparticles. Naked iron oxide nanoparticles have high chemical activity, toxicity and aggregate in the body fluid therefore providing surface coating for the stability of the magnetic nanoparticles. These protective shells not only stabilize the magnetic iron nanoparticles but also can be used for further functionalization. Here the iron oxide nanoparticles were prepared by co-precipitation method, then this nanoparticle is modified using acids- oleic acid and succinic acid and a comparative study is carried out. The TEM, FTIR and DSC characterization techniques were used to confirm the surface modification. After which, it was found the iron oxide nanoparticle with succinic acid gives a uniform coating of the three and can be used for further functionalization for various applications.

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