The Photodynamic Anti-Tumor Effects of New PPa-CDs Conjugate with pH Sensitivity and Improved Biocompatibility

2021 ◽  
Vol 21 ◽  
Author(s):  
Faiza Sajjada ◽  
Xu-Ying Liua ◽  
Yi-Jia Yanb ◽  
Xing-Ping Zhoua ◽  
Zhi-Long Chena
Keyword(s):  
Emission Spectra ◽  
Cancer Cell Line ◽  
In Vitro Cytotoxicity ◽  
Ph Sensitivity ◽  
Covalent Interaction ◽  
Esophageal Cancer Cell ◽  
Esophageal Cancer Cell Line ◽  
Good Biocompatibility ◽  
Human Esophageal Cancer

Background: Photodynamic therapy has been increasingly used to cope with the alarming problem of cancer. Porphyrins and its derivatives are widely used as potent photosensitizers (PS) for PDT. However, hydrophobicity of porphyrins poses a challenge for their use in clinics, while most of the carbon dots (CDs) are known for good biocompatibility, solubility, and pH sensitivity. Objective: To improve the properties/biocompatibility of the pyropheophorbide-α for PDT. Methods: Methods: PPa-CD conjugate was synthesized through covalent interaction using amide condensation. The structure of synthesized conjugate was confirmed by TEM, 1HNMR, and FTIR. The absorption and emission spectra were studied. In vitro, cytotoxicity of the conjugate was examined in the Human esophageal cancer cell line (Eca-109). Results: The results showed that the fluorescence of the drug was increased from its precursor. CD based conjugate could generate ROS as well as enhanced the biocompatibility by decreasing the cytotoxicity. The conjugated drug also showed pH sensitivity in different solutions. Conclusion: The dark toxicity, as well as hemocompatibility, were improved.

scholarly journals Diverse Polyphenols from Hypericum faberi

2019 ◽  
Vol 9 (3) ◽  
pp. 215-221 ◽  
Author(s):  
Xin-Wen Zhang ◽  
Yan-Song Ye ◽  
Fan Xia ◽  
Xing-Wei Yang ◽  
Gang Xu
Keyword(s):  
Cell Line ◽  
Pancreatic Tumor ◽  
Cancer Cell Line ◽  
Tumor Cell Line ◽  
Detailed Comparison ◽  
Esophageal Cancer Cell ◽  
Esophageal Cancer Cell Line ◽  
Human Esophageal Cancer ◽  
Whole Plants

Abstract Six new polyphenols with different isoprenylated xanthones, isoprenylated acylphloroglucinols, and chromone architectures, hyperfaberols A–F (1–6), were isolated from the whole plants of Hypericum faberi along with seven other related known compounds. In which hyperfaberols A/B (1/2) and 12–13 were isoprenylated xanthones, hyperfaberols C–E (3–5) and 8–11 were seven isoprenylated acylphloroglucinol derivatives, while 6–7 were two chromones. Their structures were elucidated by comprehensive analysis of their spectroscopic data as well as detailed comparison with the literature data. Compounds 1 and 11 showed cytotoxities against the human esophageal cancer cell line (ECA-109) and the pancreatic tumor cell line (PANC-1) in vitro, respectively. Graphical Abstract

Andrographolide radiosensitizes human esophageal cancer cell line ECA109 to radiationin vitro

2014 ◽  
Vol 29 (1) ◽  
pp. 54-61 ◽  
Author(s):  
Z.-M. Wang ◽  
Y.-H. Kang ◽  
X. Yang ◽  
J.-F. Wang ◽  
Q. Zhang ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Esophageal Cancer Cell ◽  
Esophageal Cancer Cell Line ◽  
Human Esophageal Cancer

scholarly journals Synthesis of Amino Acid Schiff Base Nickel (II) Complexes as Potential Anticancer Drugs In Vitro

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Yang Li ◽  
Jianfang Dong ◽  
Peiran Zhao ◽  
Ping Hu ◽  
Dawei Yang ◽  
...  
Keyword(s):  
Schiff Base ◽  
Anticancer Agents ◽  
Cancer Cell Line ◽  
Dependent Manner ◽  
X Ray Crystallography ◽  
M Phase ◽  
Ic50 Values ◽  
Esophageal Cancer Cell ◽  
Esophageal Cancer Cell Line

Three hexacoordinated octahedral nickel (II) complexes, [Ni (Trp-sal) (phen) (CH3OH)] (1), [Ni (Trp-o-van) (phen) (CH3OH)]•2CH3OH (2), and [Ni (Trp-naph) (phen) (CH3OH)] (3) (where Trp-sal = Schiff base derived from tryptophan and salicylaldehyde, Trp-o-van = Schiff base derived from tryptophan and o-vanillin, Trp-naph = Schiff base derived from tryptophan and 2-hydroxy-1-naphthaldehyde, phen = 1, 10-phenanthroline), have been synthesized and characterized as potential anticancer agents. Details of structural study of these complexes using single-crystal X-ray crystallography showed that distorted octahedral environment around nickel (II) ion has been satisfied by three nitrogen atoms and three oxygen atoms. All these complexes displayed moderate cytotoxicity toward esophageal cancer cell line Eca-109 with the IC50 values of 23.95 ± 2.54 μM for 1, 18.14 ± 2.39 μM for 2, and 21.89 ± 3.19 μM for 3. Antitumor mechanism studies showed that complex 2 can increase the autophagy, reactive oxygen species (ROS) levels, and decrease the mitochondrial membrane potential remarkably in a dose-dependent manner in the Eca-109 cells. Complex 2 can cause cell cycle arrest in the G2/M phase. Additionally, complex 2 can regulate the Bcl-2 family and autophagy-related proteins.

scholarly journals Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Faiza Sajjad ◽  
Yi-Jia Yan ◽  
Davor Margetić ◽  
Zhi-Long Chen
Keyword(s):  
Cancer Cell Line ◽  
Ros Generation ◽  
One Pot ◽  
Red Emission ◽  
Vis Spectroscopy ◽  
Polymer Dots ◽  
Esophageal Cancer Cell ◽  
Esophageal Cancer Cell Line ◽  
Carbon Based

AbstractCarbon based polymer dots have piqued attention of researchers because of excellent biocompatibility, and good solubility. Most of the p-dots are able to generate ROS which is effective for photodynamic therapy for the treatment of cancer while some photosensitizers such as porphyrins possess some drawbacks such as hydrophobicity, and dark toxicity. Therefore in this study we conjugated red emission carbon based polymer with pyropheophorbide-α through amide condensation and π–π stacking. One pot synthesis of the conjugate was successfully achieved. Their photophysiological properties were studied and structures were characterized by FT-IR, TEM and 1HNMR. pH- sensitivity of the conjugates was confirmed using fluorescence and UV–vis spectroscopy. Photo toxicity and dark toxicity of the prepared conjugates were evaluated in human esophageal cancer cell line (Eca-109). Hemocompatibility of the synthesized conjugates was evaluated and proved that the conjugates are safe to use for the treatment of tumor. Our results showed the PS doped p-dots had less dark toxicity and increased light toxicity as well as ROS generation was high as compared to precursor drug. Therefore, incorporation of p-dots to porphyrin improved biocompatibility and enhanced the photodynamic effect.

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