Benfotiamine reduces dendritic cell inflammatory potency

2020 ◽  
Vol 20 ◽  
Author(s):  
Neda Djedovic ◽  
Iva Božić ◽  
Đorđe Miljković ◽  
Irena Lavrnja
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Immune Response ◽  
Dendritic Cells ◽  
Mhc Class Ii ◽  
Morphological Changes ◽  
Marrow Cell ◽  
Class Ii ◽  
Immunofluorescent Staining ◽  
Mhc Class Ii Molecules

Background: Benfotiamine is a synthetic liposoluble derivative of vitamin B1 that has been shown to have antiinflammatory properties. Objective: To study the effects of benfotiamine on dendritic cells. Methods: Dendritic cells were obtained from murine bone marrow precursor cells in the presence of GM-CSF. Benfotiamine was applied to the cell culture during the process of bone marrow cell differentiation into dendritic cells. Dendritic cells were stimulated with lipopolysaccharide (LPS) and expression of MHC class II molecules and CD86 was determined by flow cytometry, while levels of tumor necrosis factor (TNF) and interleukin (IL)-1β in cell culture supernatants were measured by ELISA. F-Actin, NF-κB and Nrf2 were visualized by immunofluorescent staining and microscopy. Results: Benfotiamine potently reduced LPS-induced expression of MHC class II molecules and CD86, in addition to suppressing the release of pro-inflammatory cytokines TNF and IL-1β. It also prevented LPS-imposed morphological changes of dendritic cells, i.e. enlargement and intensified protrusions. The effects were paralleled with the reduction of NF-κB translocation to the nucleus, but not of Nrf2 activation inhibition. Conclusion: Having in mind the importance of dendritic cells for the configuration of the immune response, our results imply that benfotiamine has the ability to regulate the immune response through inhibition of inflammatory properties of dendritic cells.

scholarly journals Activation of Human Dendritic Cells Is Modulated by Components of the Outer Membranes of Neisseria meningitidis

2003 ◽  
Vol 71 (10) ◽  
pp. 5590-5597 ◽  
Author(s):  
Tamara Al-Bader ◽  
Myron Christodoulides ◽  
John E. Heckels ◽  
Judith Holloway ◽  
Amanda E. Semper ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Neisseria Meningitidis ◽  
Mrna Expression ◽  
Mhc Class Ii ◽  
Human Monocyte ◽  
Class Ii ◽  
Effective Vaccine ◽  
Tlr4 Mrna ◽  
Mhc Class Ii Molecules

ABSTRACT Neisseria meningitidis serogroup B is a major cause of life-threatening meningitis and septicemia worldwide, and no effective vaccine is available. Initiation of innate and acquired immune responses to N. meningitidis is likely to be dependent on cellular responses of dendritic cells (DC) to antigens present in the outer membrane (OM) of the meningococcus. In this study, the responses of human monocyte-derived DC (mo-DC) to OM isolated from parent (lipopolysaccharide [LPS]-replete) meningococci and from a mutant deficient in LPS were investigated. Parent OM selectively up-regulated Toll-like receptor 4 (TLR4) mRNA expression and induced mo-DC maturation, as reflected by increased production of chemokines, proinflammatory cytokines, and CD83, CD80, CD86, CD40, and major histocompatibility complex (MHC) class II molecules. In contrast, LPS-deficient OM selectively up-regulated TLR2 mRNA expression and induced moderate increases in both cytokine production and expression of CD86 and MHC class II molecules. Preexposure to OM, with or without LPS, augmented the allostimulatory properties of mo-DC, which induced proliferation of naive CD4+ CD45RA+ T cells. In addition, LPS-replete OM induced a greater gamma interferon/interleukin-13 ratio in naive T cells, whereas LPS-deficient OM induced the reverse profile. These data demonstrate that components of the OM, other than LPS, are also likely to be involved in determining the levels of DC activation and the nature of the T-helper immune response.

scholarly journals Major Histocompatibility Complex Class II-Independent Generation of Neutralizing Antibodies against T-Cell-Dependent Borrelia burgdorferi Antigens Presented by Dendritic Cells: Regulation by NK and γδ T Cells

2001 ◽  
Vol 69 (4) ◽  
pp. 2407-2415 ◽  
Author(s):  
M. Lamine Mbow ◽  
Nordin Zeidner ◽  
Robert D. Gilmore ◽  
Marc Dolan ◽  
Joseph Piesman ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Major Histocompatibility Complex ◽  
Borrelia Burgdorferi ◽  
Mhc Class Ii ◽  
Γδ T Cells ◽  
Humoral Response ◽  
Class Ii ◽  
Histocompatibility Complex ◽  
Mhc Class Ii Molecules

ABSTRACT We previously showed that adoptive transfer of Borrelia burgdorferi-pulsed dendritic cells (DCs) into syngeneic mice protects animals from challenge with tick-transmitted spirochetes. Here, we demonstrate that the protective immune response is antibody (Ab) dependent and does not require the presence of major histocompatibility complex (MHC) class II molecules on DCs. Mice sensitized with B. burgdorferi-pulsed MHC class II-deficient (MHC class II−/−) DCs mounted a humoral response against protective antigens, includingB. burgdorferi outer surface protein A (OspA) and OspC. B-cell help for the generation of neutralizing anti-OspC immunoglobulin G Abs could be provided by γδ T cells. In contrast, anti-OspA Ab production required the presence of αβ T cells, although this pathway could be independent of MHC class II molecules on antigen-presenting cells. Moreover, depletion of NK cells prior to transfer of antigen-pulsed MHC class II−/− DCs resulted in significant increases in the levels of neutralizing Abs induced by DCs. Altogether, these data suggest that the initial interactions between DCs and innate immune cells, such as γδ and NK cells, can influence the generation of a protective humoral response againstB. burgdorferi antigens.

ATPase and MHC class II molecules co-expression in Rana pipiens dendritic cells

1999 ◽  
Vol 23 (6) ◽  
pp. 473-485 ◽  
Author(s):  
Andrés E Castell-Rodrı́guez ◽  
Alberto Hernández-Peñaloza ◽  
Enrique A Sampedro-Carrillo ◽  
Miguel A Herrera-Enriquez ◽  
Sara J Alvarez-Pérez ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Mhc Class Ii ◽  
Rana Pipiens ◽  
Class Ii ◽  
Mhc Class Ii Molecules

scholarly journals Further Increase in the Expression of Activation Markers on Monocyte-Derived Dendritic Cells in Coronary Artery Disease Patients with Ectasia Compared to Patients with Coronary Artery Disease Alone

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Nesligul Yildirim ◽  
Ishak Ozel Tekin ◽  
Mehmet Arasli ◽  
Mustafa Aydin
Keyword(s):  
Coronary Artery Disease ◽  
Dendritic Cells ◽  
Coronary Artery ◽  
Mhc Class Ii ◽  
Coronary Arteries ◽  
Class Ii ◽  
Blood Monocytes ◽  
Activation Markers ◽  
Artery Disease ◽  
Mhc Class Ii Molecules

Background. Coronary artery ectasia (CAE) is defined as localized or diffuse dilation of the coronary arteries. There are scarce data about the role of dendritic cells in CAE development. In this study we investigated the activation markers on the surface of monocyte-derived dendritic cells (mDCs) in coronary artery disease (CAD) patients with or without CAE.Method. The study consisted of 6 patients who had obstructive CAD with CAE, 6 CAD patients without CAE and 6 subjects with angiographically normal coronary arteries. mDCs were cultivated from peripheral blood monocytes. Surface activation markers were detected by flow cytometry.Results. CAD patients with CAE were detected to have significantly higher mean fluorescence intensities of CD11b, CD11c, CD54 , CD83, CD86 and MHC Class II molecules on mDCs in comparison to CAD patients without CAE and normal controls ( for all). A significant positive correlation was found between the number of vessels with CAE and the levels of CD11c, CD86, and MHC Class II molecules.Conclusion. mDCs display an increased cell surface concentration of activation molecules in CAD patients with CAE compared to patients with CAD alone. DC activation may play an important role for CAE development in patients with CAD.

Intracellular pathway of MHC class II molecules in human dendritic cells

1996 ◽  
Vol 47 (1-2) ◽  
pp. 91
Author(s):  
J. Salamero ◽  
C. Saudrais ◽  
D. Spehner ◽  
H. de la Salle ◽  
A. Bohbot ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Human Dendritic Cells ◽  
Intracellular Pathway ◽  
Mhc Class Ii Molecules

Generation of murine dendritic cells from flt3-ligand–supplemented bone marrow cultures

Blood ◽  
2000 ◽  
Vol 96 (9) ◽  
pp. 3029-3039 ◽  
Author(s):  
Kenneth Brasel ◽  
Thibaut De Smedt ◽  
Jeffery L. Smith ◽  
Charles R. Maliszewski
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Mhc Class Ii ◽  
Culture System ◽  
Antigen Processing ◽  
Class Ii ◽  
Interferon Α ◽  
Flt3 Ligand ◽  
Gm Csf

Abstract Murine dendritic cells (DCs) can be classified into at least 2 subsets, “myeloid-related” (CD11bbright, CD8α−) and “lymphoid-related” (CD11bdull, CD8α+), but the absolute relationship between the 2 remains unclear. Methods of generating DCs from bone marrow (BM) precursors in vitro typically employ granulocyte-macrophage colony-stimulating factor (GM-CSF) as the principal growth factor, and the resultant DCs exhibit a myeloidlike phenotype. Here we describe a flt3-ligand (FL)–dependent BM culture system that generated DCs with more diverse phenotypic characteristics. Murine BM cells cultured at high density in recombinant human FL for 9 days developed into small lymphoid-sized cells, most of which expressed CD11c, CD86, and major histocompatibility complex (MHC) class II. The CD11c+ population could be divided into 2 populations on the basis of the level of expression of CD11b, which may represent the putative myeloid- and lymphoid-related subsets. The FL in vitro–derived DCs, when treated with interferon-α or lipopolysaccharide during the final 24 hours of culture, expressed an activated phenotype that included up-regulation of MHC class II, CD1d, CD8α, CD80, CD86, and CD40. The FL-derived DCs also exhibited potent antigen-processing and antigen-presenting capacity. Neutralizing anti–interleukin-6 (IL-6) antibody, but not anti–GM-CSF, significantly reduced the number of DCs generated in vitro with FL, suggesting that IL-6 has a role in the development of DCs from BM precursors. Stem cell factor, which exhibits some of the same bioactivities as FL, was unable to replace FL to promote DC development in vitro. This culture system will facilitate detailed analysis of murine DC development.

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