Effects of Histidine-Tag on Recombinant Human Cytochrome P450 3A5 Catalytic Activity in Reconstitution Systems

2009 ◽  
Vol 3 (4) ◽  
pp. 207-211 ◽  
Author(s):  
Chie Emoto ◽  
Norie Murayama ◽  
Sachiko Wakiya ◽  
Hiroshi Yamazaki
Keyword(s):  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Human Cytochrome ◽  
Cytochrome P450 3A5 ◽  
Histidine Tag

scholarly journals Screening of potential substrates or inhibitors of cytochrome P450 17a1 (CYP17a1) by electrochemical methods

2011 ◽  
Vol 57 (4) ◽  
pp. 402-409 ◽  
Author(s):  
V.V. Shumyantseva ◽  
T.V. Bulko ◽  
A.Yu. Misharin ◽  
A.I. Archakov
Keyword(s):  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Electrochemical Reduction ◽  
Cytochromes P450 ◽  
Electrochemical Behaviour ◽  
Differential Pulse ◽  
Synthetic Surfactant ◽  
Catalytic Current ◽  
Human Cytochrome ◽  
Substrate Properties

The electrochemical reduction of the recombinant form of human cytochrome P450 17A1 (CYP17A1) was investigated. Hemeprotein was immobilized on electrode modified with biocompatable nanocomposite material based on the membrane-like synthetic surfactant didodecyldimethylammonium bromide (DDAB) and gold nanoparticles. Analytical characteristics of DDAB/Au/CYP17A1 electrodes were investigated with cyclic voltammetry, square wave voltammetry, and differential pulse voltammetry. Analysis of electrochemical behaviour of cytochrome P450 17A1 was conducted in the presence of substrate pregnenolone (1), inhibitor ketoconazole (2), and in the presence of synthetic derivatives of pregnenolone: acetylpregnenolone (3), cyclopregnenolone (4), and tetrabrompregnenolone (5). Ketoconazole, azole inhibitor of cytochromes P450, blocked catalytic current in the presence of substrate pregnenolone (1). Compounds 3-5 did not demonstrate substrate properties towards electrode/CYP17A1 system. Compound 3 did not block catalytic activity towards pregnenolone, but compounds 4 and 5 inhibited such activity. Electrochemical reduction of CYP17A1 may serve as an adequate substitution of the reconstituted system which requires additional redox partners - for the exhibition of catalytic activity of hemoproteins of the cytochrome P450 superfamily.

A Naturally Occurring Isoform-Specific Probe for Highly Selective and Sensitive Detection of Human Cytochrome P450 3A5

2017 ◽  
Vol 60 (9) ◽  
pp. 3804-3813 ◽  
Author(s):  
Jing-Jing Wu ◽  
Yun-Feng Cao ◽  
Liang Feng ◽  
Yu-Qi He ◽  
James Y. Hong ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Sensitive Detection ◽  
Specific Probe ◽  
Naturally Occurring ◽  
Human Cytochrome ◽  
Cytochrome P450 3A5

scholarly journals Clobetasol Propionate Is a Heme-Mediated Selective Inhibitor of Human Cytochrome P450 3A5

2020 ◽  
Vol 63 (3) ◽  
pp. 1415-1433 ◽  
Author(s):  
William C. Wright ◽  
Jude Chenge ◽  
Jingheng Wang ◽  
Hazel M. Girvan ◽  
Lei Yang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Selective Inhibitor ◽  
Clobetasol Propionate ◽  
Human Cytochrome ◽  
Cytochrome P450 3A5
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