scholarly journals Sample Preparation Overview for the Chromatographic Determination of 1,4-Benzodiazepines in Biological matrices

2012 ◽  
pp. 84-107
Keyword(s):  
Sample Preparation ◽  
Chromatographic Determination ◽  
Biological Matrices

scholarly journals Overview of the analytical methods for vancomycin and/or teicoplanin determination in biological matrices

2017 ◽  
Vol 71 (2) ◽  
pp. 87-97 ◽  
Author(s):  
Ana Stajic ◽  
Biljana Jancic-Stojanovic
Keyword(s):  
Sample Preparation ◽  
Bacterial Infections ◽  
Multidrug Resistant ◽  
Glycopeptide Antibiotics ◽  
Biological Matrices ◽  
Preparation Methods ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Improved Methods

Background. Teicoplanin and vancomycin are glycopeptide antibiotics currently in use for treatment of multidrug-resistant bacterial infections. Scope and Approach. Severe undesirable effects, such as ototoxicity, nephrotoxicity and neutropenia have been reported for vancomycin and teicoplanin, which necessitates monitoring the concentration of these two drugs in different biological samples. In order to obtain precise and accurate results, sensitive, reliable and fast methods are necessary. The main aim of this mini review is to give a clear and concise overview of the recently developed, validated, novel and improved methods for glycopeptide antibiotic analyses in various biological matrices. Also, the variability of the matrices requires optimal and effective sample preparation procedures to be developed, and so these are discussed. Key Findings and Conclusions. Different liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods have been described for quantitative determination of glycopeptide antibiotics in various biological matrices. It was shown that protein precipitation was a convenient method for sample preparation despite the high number of novel sample preparation methods.

scholarly journals Comparison of two sample preparation procedures for HPLC determination of ochratoxin A

2009 ◽  
Vol 61 (4) ◽  
pp. 639-644 ◽  
Author(s):  
Gorica Vukovic ◽  
Snezana Pavlovic ◽  
M.S. Ristic
Keyword(s):  
Sample Preparation ◽  
Phosphoric Acid ◽  
Ochratoxin A ◽  
Chromatographic Determination ◽  
Liquid Extraction ◽  
Immunoaffinity Column ◽  
Maize Flour ◽  
Liquid Liquid Extraction ◽  
Immunoaffinity Columns

In preparation of samples for chromatographic determination of ochratoxin A, two types of columns were used for sample cleanup (SPE and immunoaffinity columns). The first method consisted of liquid-liquid extraction with a mixture of chloroform and phosphoric acid, followed by ion-exchange cleanup on Waters Oasis MAX columns. The sec?ond method consisted of extraction with a mixture of water and methanol, followed by LCTech OtaCLEAN immunoaf?finity column cleanup. Recoveries of the methods were determined at three levels in three repetitions for maize flour, and they were 84% (%RSD = 19.2) for the first method of sample preparation and 101% (%RSD = 2.2) for the second method. Values of LOQ for OTA were 0.25 and 1.00 ?g/kg for the IAC and SPE clean-up procedures, respectively. Both methods comply with present regulations, but the MAX sample clean-up procedure should be used as an alternative, since the immunoaffinity column clean-up procedure is characterized by better reproducibility, accuracy, and efficiency.

scholarly journals Analysis of melatonin and serotonin from cherries: a review

2018 ◽  
Vol 22 (1) ◽  
pp. 52-60
Author(s):  
Varga-Visi Éva ◽  
Ildikó Jócsák ◽  
Katalin Lóki ◽  
György Végvári
Keyword(s):  
Sample Preparation ◽  
Electrochemical Detection ◽  
Sweet Cherry ◽  
Sour Cherry ◽  
Chromatographic Determination ◽  
Fluorometric Detection ◽  
Liquid Chromatographic Determination ◽  
Special Respect ◽  
Liquid Chromatographic

The proper quantification of serotonin and melatonin in different foods is indispensable to evaluate the significance of their dietary intake on blood and plasma levels in humans. This review summarizes the issues regarding the measurements of these compounds from sour cherry and sweet cherry. Sample preparation and analytical methods are discussed, mainly focusing on the liquid chromatographic determination of these compounds, with special respect to their parallel determination in one run. Keywords: indoleamine derivatives, LC-MS, electrochemical detection, fluorometric detection

Direct Blood-Injection Method for Gas Chromatographic Determination of Alcohols and Other Volatile Compounds

1971 ◽  
Vol 17 (2) ◽  
pp. 82-85 ◽  
Author(s):  
Naresh C Jain
Keyword(s):  
Sample Preparation ◽  
Volatile Compounds ◽  
Low Cost ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Blood Injection

Abstract An extremely simple, rapid method is described for simultaneously determining methanol, ethanol, acetone, isopropanol, and low-boiling hydrocarbons associated with glue sniffing. Less than 1 µl of blood, mixed with an internal standard, is injected directly into a low-cost gas chromatograph equipped with a flame-ionization detector. No extraction, distillation, and (or) sample preparation is required, and the method is sensitive to less than 10 µg of alcohol per ml.

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