In vitro Physicochemical, Phytochemical, Antimicrobial and Antidiabetic studies on Mucuna pruriens (Linn.) DC seeds

2016 ◽  
Vol 5 (06) ◽  
pp. 4650 ◽  
Author(s):  
Srilakshmi Kusuma* ◽  
Aniel Kumar O. ◽  
Lakshmi Narayana K. ◽  
Sudhakar Pola ◽  
Venkata Reddy K.
Keyword(s):  
Developing Countries ◽  
Methanol Extract ◽  
Mucuna Pruriens ◽  
Bioactive Properties ◽  
Treatment Of Diabetes ◽  
Relative Deficiency ◽  
Alpha Glucosidase ◽  
Crude Methanol Extract ◽  
Seed Extracts

Diabetes is one of the most common endocrine diseases characterized by hyperglycemia due to absolute or relative deficiency of insulin which is currently affecting the citizens of both developed and developing countries. According to Williams textbook of endocrinology in 2013 it was estimated that over 382 million people throughout the world had diabetes. Plants have long been used for the treatment of diabetes, particularly in developing countries where most people have limited resources and do not have access to modern treatment. The presented study is aimed to evaluate the Mucuna pruriens seed extracts for its in vitro physiochemical, phytochemical, antioxidant and anti-diabetic studies. From the studies different solvent extracts of hexane and chloroform showed little or no activity on all assays performed whereas methanol extract of Mucuna pruriens showed significant bio properties. The preliminary studies of this plant crude methanol extract exhibited maximum compounds, hence the methanol extracts have under taken for its alpha amylase and alpha glucosidase inhibition activity. On the basis of the results obtained in the present investigation, it can be concluded that methanol extract of Mucuna pruriens had significant bioactive properties and may provide a support to use of the plant in traditional medicine for the management of diabetes.

scholarly journals Evaluating the Inhibitory Effects of Dichloromethane and Methanol Extracts of Salvia macilenta and Salvia officinalis on the Diabetes Marker Enzyme, Alpha-Glucosidase: An Approach for the Treatment of Diabetes

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Hassan Javid ◽  
Soheila Moein ◽  
Mahmood Reza Moein
Keyword(s):  
Glucose Uptake ◽  
Methanol Extract ◽  
Inhibitory Effect ◽  
Salvia Officinalis ◽  
Marker Enzyme ◽  
Inhibitory Effects ◽  
Methanol Extracts ◽  
Treatment Of Diabetes ◽  
Alpha Glucosidase

Background: Diabetes mellitus is believed to be the most serious metabolic disease. One of the treatments for diabetes is to delay glucose uptake by inhibiting carbohydrate-hydrolyzing enzymes. Alpha-glucosidase inhibitors delay glucose uptake. Objectives: The present study was conducted aiming to evaluate the efficacy of Salvia extracts in inhibiting diabetes marker enzymes and their effects on the treatment of diabetes. Methods: This experimental study was performed in vitro. The studied plants included Salvia macilenta and Salvia officinalis. The inhibitory effects of their dichloromethane and methanol extracts were also investigated. After calculating the percentage of inhibition and IC50, Km and Vmax using GraphPad Prism 7 were also calculated. The statistical analysis was performed employing GraphPad Instat 3 software. Results: The results herein showed that the greatest inhibitory effect on alpha-glucosidase belonged to the methanol extract of S. macilenta with IC50 = 8.73 ± 0.26 mg/mL compared to that of acarbose with IC50 = 8.82 ± 0.14 mg/mL as a standard. The IC50 of dichloromethane extract of S. officinalis was 8.95 ± 0.23 mg/mL. Conclusions: The extracts had significant inhibitory effects on alpha-glucosidase. However, methanol extract of S. macilenta and dichloromethane extract of S. officinalis demonstrated the greatest inhibitory effects on alpha-glucosidase compared to acarbose as a standard.

In vitro assessment of the anti-sickling properties of Buchholzia coriacea and Mucuna pruriens seed extracts

2020 ◽  
Vol 56 (9) ◽  
pp. 773-782
Author(s):  
Eberechukwu Laura Ikechukwu ◽  
Polycarp Nnacheta Okafor ◽  
Simeon Ikechukwu Egba
Keyword(s):  
Mucuna Pruriens ◽  
In Vitro Assessment ◽  
Seed Extracts

scholarly journals Antioxidant Potential, DNA Protection, and HPLC-DAD Analysis of Neglected MedicinalJurinea dolomiaeaRoots

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Naseer Ali Shah ◽  
Muhammad Rashid Khan ◽  
Kiran Naz ◽  
Mubarak Ali Khan
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Alpine Region ◽  
Total Phenolic ◽  
Dna Protection ◽  
Tuberous Roots ◽  
Gallic Acid Equivalent ◽  
Crude Methanol Extract

Jurinea dolomiaeaBoiss., family Compositae, is a medicinally important plant of alpine region. Its tuberous roots are used in various ailments in folk medicine. This study was undertaken to estimate total phenolic (TPC) and total flavonoid contents (TFC) and to determine anti-free radical potential by diversein vitroantioxidant assays. Crude methanol extract (JDME) was fractionated inton-hexane (JDHE), chloroform (JDCE), ethyl acetate (JDEE),n-butanol (JDBE), and aqueous (JDAE) fractions. The results indicated that JDEE and JDCE constituted the highest amount of TFC (807±7.2 mg rutin equivalent/g sample) and TPC (757±9.4 mg gallic acid equivalent/g sample), respectively. Significant correlation of TFC with IC50values was recorded for•OH (R2=0.91), H2O2(R2=0.82), and ABTS (R2=0.82) assay. It could be made clear that JDEE was the most potent in antioxidant activity as compared to others, with generally lower IC50values for DPPH (41.1±1.0 μg/mL), ABTS (46.7±0.6 μg/mL), H2O2(42.2±0.9 μg/mL),•OH (61.1±1.1 μg/mL),O2-(152±1.1 μg/mL), and antilipid peroxidation (54.3±1.6 μg/mL). HPLC chromatogram of JDEE revealed the presence of catechin, caffeic acid, and rutin. The results indicated the antioxidant activities ofJ. dolomiaearoots and merit further investigations for their use in oxidative stress related disorders.

scholarly journals In Vitro Screening of Seed Extracts of Medicinal Plants for Protease Inhibitory Activity

2019 ◽  
Vol 3 (1) ◽  
pp. 61-65
Author(s):  
Faiyaz Khudaboddin Shaikh ◽  
Sarwan W. Hamad ◽  
Saber W. Hamad ◽  
Ashok A. Shinde
Keyword(s):  
Medicinal Plants ◽  
Storage Proteins ◽  
Plant Protection ◽  
Mucuna Pruriens ◽  
Ocimum Sanctum ◽  
Moderate Activity ◽  
Dot Blot ◽  
Activity Data ◽  
Seed Extracts

Protease inhibitors (PIs) are deployed in the plant kingdom as storage proteins or peptides, regulators of endogenous proteases, and plant protection agents against insect pests and pathogen attack. In humans, they are identified as chemopreventive agents against a range of cancers and have potential as drug to treat an array of disease associated with aberrant activity of proteases. The present investigation reports PIs activity data from 30 medicinal plants. The screening for PIs activity was done by dot blot assay using X-ray film coated with gelatin. Among screened seed extracts, Albizia lebbeck, Raphanus sativus, Mucuna pruriens, Achyranthes aspera, and Coffea arabica showed high inhibitory activities with trypsin protease. Most of seed extracts exhibited moderate activity, whereas Ocimum sanctum showed moderate to low activity against trypsin. The presence of varied protein content is reported from all seed extracts with highest in A. lebbeck (50.0 ± 3.4 mg/ml). The data produced in the present investigation could be helpful for further exploration of PIs as therapeutic agent.

scholarly journals In vitro Investigation of Mucuna pruriens Seed Extracts to Treat Erectile Dysfunction

2018 ◽  
Vol 17 (2) ◽  
Author(s):  
Natthachai Duangnin ◽  
◽  
Thanyaluck Phitak ◽  
Peraphan Pothacharoen ◽  
Prachya Kongtawelert ◽  
...  
Keyword(s):  
Erectile Dysfunction ◽  
Mucuna Pruriens ◽  
In Vitro Investigation ◽  
Seed Extracts

scholarly journals In vitro time kill assessment of crude methanol extract of Helichrysum pedunculatum leaves

2008 ◽  
Vol 7 (11) ◽  
pp. 1684-1688 ◽  
Author(s):  
Aiyegoro ◽  
A O ◽  
Afolayan ◽  
J A ◽  
Okoh ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Time Kill ◽  
Crude Methanol Extract

Antibacterial Activity of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid Against Streptococcus mutans by In Vitro

2017 ◽  
Vol 7 (03) ◽  
Author(s):  
Sri Atun ◽  
Sri Handayani ◽  
Melia Aliffiana ◽  
Hajar Nur Afifah ◽  
Anna Rakhmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
Alginic Acid ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

In Vitro Anticoccidial Activities of the Extract and Fractions of Garcinia kola (Heckel h.) against Eimeria tenella Oocyst

2021 ◽  
Vol 15 ◽  
Author(s):  
Manji Amos Shetshak ◽  
Isa Danjuma Jatau ◽  
Muhammed Musa Suleiman ◽  
Matthew Philip Ameh ◽  
Gabriel Ada ◽  
...  
Keyword(s):  
Cardiac Glycosides ◽  
Methanol Extract ◽  
Eimeria Tenella ◽  
Phytochemical Analysis ◽  
Garcinia Kola ◽  
Soxhlet Apparatus ◽  
Anticoccidial Agent ◽  
Garcinia Kola Heckel ◽  
Crude Methanol Extract

Background: Commercial poultry farming is expanding every day and contributing to the provision of affordable and high quality protein. However, this sector is confronted with many diseases of which coccidiosis is among the most important. There are many registered patents affirming the health benefits of Garcinia kola in poultry. Objective: Evaluation of In vitro anticoccidial activities of the extracts and fractions of Garcinia kola against Eimeria tenella oocyst was carried out. Method: Fresh seeds of G. kola were collected, dried under shade at room temperature and pulverized using a mortar and a pestle. The powder was exhaustively extracted with a soxhlet apparatus using 70% methanol and the crude methanol extract (CME) was concentrated to dryness using a rotary evaporator. The CME was further partitioned using butanol, ethylacetate and n-hexane. The CME, butanol fraction (BTF), ethylacetate fraction (EAF) and hexane fraction (HXF) were concentrated in vacuo and tested for the presence of phytochemical constituents using standard procedures. Similarly the CME, butanol, ethyl acetate and hexane fractions were evaluated in vitro for oocyst sporulation inhibition. Results: Phytochemical analysis revealed the presence of cardiac glycosides, saponins, carbohydrates, steroids/triterpenes, tannins, flavonoids and alkaloids in the CME and BTF. The EAF contains all the metabolites mentioned except saponins. Similarly, HXF contains only cardiac glycosides, tannins and steroids/triterpenes. The CME and BTF caused a concentration dependent increase in the inhibition of sporulation of unsporulated oocysts of E. tenella. In the acute toxicity studies, the CME did not produce any toxic effect or mortality at doses between 10 and 5000 mg/kg. The CME was then considered safe and the LD50 was assumed to be >5000 mg/kg. Conclusion: The data obtained in this study suggest that the crude methanol extract (CME) of G. kola could be appreciable beneficial effect as an anticoccidial agent against Eimeria tenella oocyst.

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