In vitro assessment of the anti-sickling properties of Buchholzia coriacea and Mucuna pruriens seed extracts

2020 ◽  
Vol 56 (9) ◽  
pp. 773-782
Author(s):  
Eberechukwu Laura Ikechukwu ◽  
Polycarp Nnacheta Okafor ◽  
Simeon Ikechukwu Egba
Keyword(s):  
Mucuna Pruriens ◽  
In Vitro Assessment ◽  
Seed Extracts

scholarly journals In Vitro Screening of Seed Extracts of Medicinal Plants for Protease Inhibitory Activity

2019 ◽  
Vol 3 (1) ◽  
pp. 61-65
Author(s):  
Faiyaz Khudaboddin Shaikh ◽  
Sarwan W. Hamad ◽  
Saber W. Hamad ◽  
Ashok A. Shinde
Keyword(s):  
Medicinal Plants ◽  
Storage Proteins ◽  
Plant Protection ◽  
Mucuna Pruriens ◽  
Ocimum Sanctum ◽  
Moderate Activity ◽  
Dot Blot ◽  
Activity Data ◽  
Seed Extracts

Protease inhibitors (PIs) are deployed in the plant kingdom as storage proteins or peptides, regulators of endogenous proteases, and plant protection agents against insect pests and pathogen attack. In humans, they are identified as chemopreventive agents against a range of cancers and have potential as drug to treat an array of disease associated with aberrant activity of proteases. The present investigation reports PIs activity data from 30 medicinal plants. The screening for PIs activity was done by dot blot assay using X-ray film coated with gelatin. Among screened seed extracts, Albizia lebbeck, Raphanus sativus, Mucuna pruriens, Achyranthes aspera, and Coffea arabica showed high inhibitory activities with trypsin protease. Most of seed extracts exhibited moderate activity, whereas Ocimum sanctum showed moderate to low activity against trypsin. The presence of varied protein content is reported from all seed extracts with highest in A. lebbeck (50.0 ± 3.4 mg/ml). The data produced in the present investigation could be helpful for further exploration of PIs as therapeutic agent.

scholarly journals In vitro Investigation of Mucuna pruriens Seed Extracts to Treat Erectile Dysfunction

2018 ◽  
Vol 17 (2) ◽  
Author(s):  
Natthachai Duangnin ◽  
◽  
Thanyaluck Phitak ◽  
Peraphan Pothacharoen ◽  
Prachya Kongtawelert ◽  
...  
Keyword(s):  
Erectile Dysfunction ◽  
Mucuna Pruriens ◽  
In Vitro Investigation ◽  
Seed Extracts

In vitro Physicochemical, Phytochemical, Antimicrobial and Antidiabetic studies on Mucuna pruriens (Linn.) DC seeds

2016 ◽  
Vol 5 (06) ◽  
pp. 4650 ◽  
Author(s):  
Srilakshmi Kusuma* ◽  
Aniel Kumar O. ◽  
Lakshmi Narayana K. ◽  
Sudhakar Pola ◽  
Venkata Reddy K.
Keyword(s):  
Developing Countries ◽  
Methanol Extract ◽  
Mucuna Pruriens ◽  
Bioactive Properties ◽  
Treatment Of Diabetes ◽  
Relative Deficiency ◽  
Alpha Glucosidase ◽  
Crude Methanol Extract ◽  
Seed Extracts

Diabetes is one of the most common endocrine diseases characterized by hyperglycemia due to absolute or relative deficiency of insulin which is currently affecting the citizens of both developed and developing countries. According to Williams textbook of endocrinology in 2013 it was estimated that over 382 million people throughout the world had diabetes. Plants have long been used for the treatment of diabetes, particularly in developing countries where most people have limited resources and do not have access to modern treatment. The presented study is aimed to evaluate the Mucuna pruriens seed extracts for its in vitro physiochemical, phytochemical, antioxidant and anti-diabetic studies. From the studies different solvent extracts of hexane and chloroform showed little or no activity on all assays performed whereas methanol extract of Mucuna pruriens showed significant bio properties. The preliminary studies of this plant crude methanol extract exhibited maximum compounds, hence the methanol extracts have under taken for its alpha amylase and alpha glucosidase inhibition activity. On the basis of the results obtained in the present investigation, it can be concluded that methanol extract of Mucuna pruriens had significant bioactive properties and may provide a support to use of the plant in traditional medicine for the management of diabetes.

In vitro assessment of Labisia pumila and its constituents for interactions with CYPs, P-GP and PXR

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
VK Manda ◽  
OR Dale ◽  
C Awortwe ◽  
Z Ali ◽  
IA Khan ◽  
...  
Keyword(s):  
In Vitro Assessment ◽  
Labisia Pumila

Detoxifying Action of Aqueous Extracts of Mucuna pruriens Seed and Mimosa pudica Root Against Venoms of Naja nigricollis and Bitis arietans

2020 ◽  
Vol 14 (2) ◽  
pp. 134-144 ◽  
Author(s):  
Matthew P. Ameh ◽  
Mamman Mohammed ◽  
Yusuf P. Ofemile ◽  
Magaji G. Mohammed ◽  
Ada Gabriel ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Plant Extracts ◽  
Fibrinolytic Activity ◽  
Neglected Tropical Diseases ◽  
World Health ◽  
Mucuna Pruriens ◽  
Phytochemical Analysis ◽  
Mimosa Pudica ◽  
Health Organization

Background: The World Health Organization included snakebite envenomation among Neglected Tropical Diseases in 2017. The importance of natural products from plants is enormous, given that most prescribed drugs originate from plants. Among this is Mucuna pruriens and Mimosa pudica, with many registered patents asserting their health benefits. Objective: This study investigated the in vitro neutralizing effects of Mucuna pruriens seed and Mimosa pudica root extracts on venoms of Naja nigricollis and Bitis arietans. Methods: In mice, the LD50 and phytochemical analysis of M. pruriens and M. pudica plant extracts were carried out prior to the evaluation of their haemolytic and fibrinolytic effect. Their effects on the activities of phospholipase A2 (PLA2) were also assessed. Results: At a concentration of 50 mg/ml, both plant extracts were found to neutralize the fibrinolytic activity of N. nigricollis, but 400 mg/ml was required to neutralize the fibrinolytic activity of B. arietans. In haemolytic studies, 50 mg/ml concentration of M. pruriens extract suppressed haemolysis caused by N. nigricollis venom by 70% but at the same concentration, M. pudica extract reduced haemolysis by 49.4%. M. pruriens, at 50 mg/ml concentration, only inhibited phospholipase A2 activity by 7.7% but higher concentrations up to 400mg/ml had no effect against the venom of N. nigricollis; at 200 mg/ml. M. pudica extract inhibited PLA2 activity by 23%. Conclusion: The results suggest that M. pruriens and M. pudica may be considered as promising antivenom agents for people living in a snake-bite prone environment.

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