In vitro evaluation of the pharmacological properties of crude methanol extract and its fractions of Aconitum austrokoreense aerial parts

AbstractDeoxypodophyllotoxin content of the aerial parts of Anthriscus sylvestris Hoffm. growing at different altitudes was evaluated in comparison to the roots. The lignan accumulation in ground parts was at least double compared to aerial ones. In addition antioxidant-guided fractionation of the crude methanol extract of aerial parts was performed with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. Active fractions contained mainly luteolin-7-O-glucoside and chlorogenic acid. Antioxidant properties of both crude extract and isolated compounds were also investigated with the Briggs-Rauscher (BR) oscillating reaction. A satisfactory agreement between the results obtained with the two methods was observed.

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Antioxidant Potential, DNA Protection, and HPLC-DAD Analysis of Neglected MedicinalJurinea dolomiaeaRoots

BioMed Research International ◽

10.1155/2014/726241 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 16

Author(s):

Naseer Ali Shah ◽

Muhammad Rashid Khan ◽

Kiran Naz ◽

Mubarak Ali Khan

Keyword(s):

Methanol Extract ◽

Antioxidant Activities ◽

Folk Medicine ◽

Alpine Region ◽

Total Phenolic ◽

Dna Protection ◽

Tuberous Roots ◽

Gallic Acid Equivalent ◽

Crude Methanol Extract

Jurinea dolomiaeaBoiss., family Compositae, is a medicinally important plant of alpine region. Its tuberous roots are used in various ailments in folk medicine. This study was undertaken to estimate total phenolic (TPC) and total flavonoid contents (TFC) and to determine anti-free radical potential by diversein vitroantioxidant assays. Crude methanol extract (JDME) was fractionated inton-hexane (JDHE), chloroform (JDCE), ethyl acetate (JDEE),n-butanol (JDBE), and aqueous (JDAE) fractions. The results indicated that JDEE and JDCE constituted the highest amount of TFC (807±7.2 mg rutin equivalent/g sample) and TPC (757±9.4 mg gallic acid equivalent/g sample), respectively. Significant correlation of TFC with IC50values was recorded for•OH (R2=0.91), H2O2(R2=0.82), and ABTS (R2=0.82) assay. It could be made clear that JDEE was the most potent in antioxidant activity as compared to others, with generally lower IC50values for DPPH (41.1±1.0 μg/mL), ABTS (46.7±0.6 μg/mL), H2O2(42.2±0.9 μg/mL),•OH (61.1±1.1 μg/mL),O2-(152±1.1 μg/mL), and antilipid peroxidation (54.3±1.6 μg/mL). HPLC chromatogram of JDEE revealed the presence of catechin, caffeic acid, and rutin. The results indicated the antioxidant activities ofJ. dolomiaearoots and merit further investigations for their use in oxidative stress related disorders.

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In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss

Food Control ◽

10.1016/j.foodcont.2006.04.002 ◽

2007 ◽

Vol 18 (7) ◽

pp. 800-805 ◽

Cited By ~ 196

Author(s):

F. Sharififar ◽

M.H. Moshafi ◽

S.H. Mansouri ◽

M. Khodashenas ◽

M. Khoshnoodi

Keyword(s):

Essential Oil ◽

Methanol Extract ◽

Antioxidant Activities ◽

Zataria Multiflora ◽

In Vitro Evaluation

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In Vitro and In Silico Evaluation for the Inhibitory Action of O. basilicum Methanol Extract on α-Glucosidase and α-Amylase

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/5515775 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Siba Shanak ◽

Najlaa Bassalat ◽

Raghad Albzoor ◽

Sleman Kadan ◽

Hilal Zaid

Keyword(s):

Caffeic Acid ◽

In Silico ◽

Type Ii Diabetes ◽

Methanol Extract ◽

Inhibitory Effect ◽

Major Health ◽

In Silico Docking ◽

Aerial Parts ◽

Health Complications

Diabetes mellitus is a metabolic disease that predominates, nowadays. It causes hyperglycemia and consequently major health complications. Type II diabetes is the most common form and is a result of insulin resistance in the target tissues. To treat this disease, several mechanisms have been proposed. The most direct route is via inhibiting the intestinal enzymes, e.g., α-glucosidase and α-amylase, responsible for intestinal polysaccharide digestion that therefore would reduce the absorption of monosugars through the intestinal walls. In this study, we shed the light on this route by testing the inhibitory effect of Ocimum basilicum extract on the enzymes α-glucosidase and α-amylase in vitro and in silico. Experimental procedures were performed to test the effect of the O. basilicum methanol extract from aerial parts followed by the in silico docking. 500 μg/mL of the extract led to 70.2% ± 8.6 and 25.4% ± 3.3 inhibition on α-glucosidase and α-amylase activity, respectively. Similarly, the effect of caffeic acid, a major extract ingredient, was also tested, and it caused 42.7% ± 3.0 and 47.1% ± 4.0 inhibition for α-amylase and α-glucosidase, respectively. Docking experiments were performed to predict the phytochemicals responsible for this robust inhibitory activity in the O. basilicum extracts. Several compounds have shown variable levels of inhibition, e.g., caffeic acid, pyroglutamic acid, and uvasol. The results indicated that O. basilicum can be a potent antidiabetic drug.

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In vitro evaluation of antifungal activity of aerial parts of medicinal plants Balanites aegyptiaca Del. and Spilanthes acmella Murr.

Journal of Applied Pharmaceutical Science ◽

10.7324/japs.2014.40121 ◽

2014 ◽

Vol 4 (1) ◽

pp. 123-127 ◽

Cited By ~ 1

Keyword(s):

Antifungal Activity ◽

Medicinal Plants ◽

Balanites Aegyptiaca ◽

In Vitro Evaluation ◽

Aerial Parts ◽

Spilanthes Acmella

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In vitro time kill assessment of crude methanol extract of Helichrysum pedunculatum leaves

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb08.055 ◽

2008 ◽

Vol 7 (11) ◽

pp. 1684-1688 ◽

Cited By ~ 5

Author(s):

Aiyegoro ◽

A O ◽

Afolayan ◽

J A ◽

Okoh ◽

...

Keyword(s):

Methanol Extract ◽

Time Kill ◽

Crude Methanol Extract

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Antibacterial Activity of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid Against Streptococcus mutans by In Vitro

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v7i03.9554 ◽

2017 ◽

Vol 7 (03) ◽

Author(s):

Sri Atun ◽

Sri Handayani ◽

Melia Aliffiana ◽

Hajar Nur Afifah ◽

Anna Rakhmawati

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Agent ◽

Methanol Extract ◽

Alginic Acid ◽

Ethanol Extract ◽

Diffusion Method ◽

E Coli ◽

Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

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In vitro Physicochemical, Phytochemical, Antimicrobial and Antidiabetic studies on Mucuna pruriens (Linn.) DC seeds

International Journal of Bioassays ◽

10.21746/ijbio.2016.06.0012 ◽

2016 ◽

Vol 5 (06) ◽

pp. 4650 ◽

Cited By ~ 1

Author(s):

Srilakshmi Kusuma* ◽

Aniel Kumar O. ◽

Lakshmi Narayana K. ◽

Sudhakar Pola ◽

Venkata Reddy K.

Keyword(s):

Developing Countries ◽

Methanol Extract ◽

Mucuna Pruriens ◽

Bioactive Properties ◽

Treatment Of Diabetes ◽

Relative Deficiency ◽

Alpha Glucosidase ◽

Crude Methanol Extract ◽

Seed Extracts

Diabetes is one of the most common endocrine diseases characterized by hyperglycemia due to absolute or relative deficiency of insulin which is currently affecting the citizens of both developed and developing countries. According to Williams textbook of endocrinology in 2013 it was estimated that over 382 million people throughout the world had diabetes. Plants have long been used for the treatment of diabetes, particularly in developing countries where most people have limited resources and do not have access to modern treatment. The presented study is aimed to evaluate the Mucuna pruriens seed extracts for its in vitro physiochemical, phytochemical, antioxidant and anti-diabetic studies. From the studies different solvent extracts of hexane and chloroform showed little or no activity on all assays performed whereas methanol extract of Mucuna pruriens showed significant bio properties. The preliminary studies of this plant crude methanol extract exhibited maximum compounds, hence the methanol extracts have under taken for its alpha amylase and alpha glucosidase inhibition activity. On the basis of the results obtained in the present investigation, it can be concluded that methanol extract of Mucuna pruriens had significant bioactive properties and may provide a support to use of the plant in traditional medicine for the management of diabetes.

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In Vitro Anticoccidial Activities of the Extract and Fractions of Garcinia kola (Heckel h.) against Eimeria tenella Oocyst

Recent Patents on Biotechnology ◽

10.2174/1872208315666210129095213 ◽

2021 ◽

Vol 15 ◽

Author(s):

Manji Amos Shetshak ◽

Isa Danjuma Jatau ◽

Muhammed Musa Suleiman ◽

Matthew Philip Ameh ◽

Gabriel Ada ◽

...

Keyword(s):

Cardiac Glycosides ◽

Methanol Extract ◽

Eimeria Tenella ◽

Phytochemical Analysis ◽

Garcinia Kola ◽

Soxhlet Apparatus ◽

Anticoccidial Agent ◽

Garcinia Kola Heckel ◽

Crude Methanol Extract

Background: Commercial poultry farming is expanding every day and contributing to the provision of affordable and high quality protein. However, this sector is confronted with many diseases of which coccidiosis is among the most important. There are many registered patents affirming the health benefits of Garcinia kola in poultry. Objective: Evaluation of In vitro anticoccidial activities of the extracts and fractions of Garcinia kola against Eimeria tenella oocyst was carried out. Method: Fresh seeds of G. kola were collected, dried under shade at room temperature and pulverized using a mortar and a pestle. The powder was exhaustively extracted with a soxhlet apparatus using 70% methanol and the crude methanol extract (CME) was concentrated to dryness using a rotary evaporator. The CME was further partitioned using butanol, ethylacetate and n-hexane. The CME, butanol fraction (BTF), ethylacetate fraction (EAF) and hexane fraction (HXF) were concentrated in vacuo and tested for the presence of phytochemical constituents using standard procedures. Similarly the CME, butanol, ethyl acetate and hexane fractions were evaluated in vitro for oocyst sporulation inhibition. Results: Phytochemical analysis revealed the presence of cardiac glycosides, saponins, carbohydrates, steroids/triterpenes, tannins, flavonoids and alkaloids in the CME and BTF. The EAF contains all the metabolites mentioned except saponins. Similarly, HXF contains only cardiac glycosides, tannins and steroids/triterpenes. The CME and BTF caused a concentration dependent increase in the inhibition of sporulation of unsporulated oocysts of E. tenella. In the acute toxicity studies, the CME did not produce any toxic effect or mortality at doses between 10 and 5000 mg/kg. The CME was then considered safe and the LD50 was assumed to be >5000 mg/kg. Conclusion: The data obtained in this study suggest that the crude methanol extract (CME) of G. kola could be appreciable beneficial effect as an anticoccidial agent against Eimeria tenella oocyst.

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