Low-Cost, Low-Tech Biosolids Treatment via Long-Term Storage: A Comparison of Two Pilot-Scale Studies

The ‘Nijisseiki’ cultivar of Japanese pears (Pyrus pyrifolia) is also known as nashi in Australia. Nashi were exposed to levels of <0.005, 0.01, 0.1 and 1.0 µL/L of ethylene in air during 26 weeks storage at 0°C. Levels of ethylene as low as 0.01 µL/L increased chlorophyll loss and visual green colour. Increasing ethylene levels also increased softening and internal browning, although flesh spot decay was reduced in the presence of ethylene. While it would be worthwhile to remove ethylene during long-term storage of ‘Nijisseiki’ in air, another alternative, adding 2% carbon dioxide to the atmosphere, is suggested as a possible low cost means to overcome the ripening effect of ethylene.

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Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix

10.1101/163212 ◽

2017 ◽

Author(s):

Megan M. Thompson ◽

Estelle M. Hrabak

Keyword(s):

Arabidopsis Thaliana ◽

Nucleic Acids ◽

Plant Species ◽

Efficient Method ◽

Genomic Dna ◽

Low Cost ◽

Long Term Storage ◽

Cellulose Matrix ◽

Term Storage

AbstractWhatman FTA ®Cards are a fast and efficient method for capturing and storing nucleic acids but are cost-prohibitive for some researchers. We developed a method that substitutes readily-available cellulose-based paper and homemade washing buffer for commercial FTA ®Cards and FTA ®Purification Reagent. This method is suitable for long-term storage of DNA from many plant species, including Arabidopsis thaliana, prior to purification and PCR.Method SummaryHere we report a low-cost method for long-term storage of plant genomic DNA on a readily available cellulose matrix.

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Evaluation of a low-cost procedure for sampling, long-term storage, and extraction of RNA from blood for qPCR analyses

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2014-1054 ◽

2015 ◽

Vol 53 (8) ◽

Cited By ~ 6

Author(s):

Rasmus B. Mærkedahl ◽

Hanne Frøkiær ◽

Lotte Lauritzen ◽

Stine B. Metzdorff

Keyword(s):

Gene Expression ◽

Clinical Trials ◽

Low Cost ◽

Rna Extraction ◽

Initial Step ◽

Rna Integrity ◽

Long Term Storage ◽

Significant Cost Reduction ◽

Term Storage

AbstractIn large clinical trials, where RNA cannot be extracted immediately after sampling, preserving RNA in whole blood is a crucial initial step in obtaining robust qPCR data. The current golden standard for RNA preservation is costly and designed for time-consuming column-based RNA-extraction. We investigated the use of lysis buffer for long-term storage of blood samples for qPCR analysis.Blood was collected from 13 healthy adults and diluted in MagMAX lysis/binding solution or PAXgene Blood RNA tubes and stored at –20 °C for 0, 1, or 4 months before RNA extraction by the matching method. RNA integrity, yield and purity were evaluated and the methods were compared by subsequent analyses of the gene expression levels ofThe MagMAX system extracted 2.3–2.8 times more RNA per mL blood, with better performance in terms of purity, and with comparable levels of integrity relative to the PAXgene system. Gene expression analysis using qPCR of: The MagMAX system can be used for storage of human blood for up to 4 months and is equivalent to the PAXgene system for RNA extraction. It furthermore, provides a means for significant cost reduction in large clinical trials.

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Reactivation and pilot-scale application of long-term storage denitrification biofilm based on flow cytometry

Water Research ◽

10.1016/j.watres.2018.10.072 ◽

2019 ◽

Vol 148 ◽

pp. 368-377 ◽

Cited By ~ 12

Author(s):

Shuo Wang ◽

Kai Qian ◽

Yin Zhu ◽

Xuesong Yi ◽

Guangsheng Zhang ◽

...

Keyword(s):

Flow Cytometry ◽

Pilot Scale ◽

Long Term Storage ◽

Term Storage

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Long-Term Storage of Small Natural History Specimens Using Gelatin Capsules: A Case Study from the Royal Alberta Museum

Collection Forum ◽

10.14351/0831-4985-32.1.31 ◽

2018 ◽

Vol 32 (1) ◽

pp. 31-46

Author(s):

Diana Tirlea ◽

Carmen Li ◽

Alwynne B. Beaudoin ◽

Emily Moffat

Keyword(s):

Continuous Monitoring ◽

Low Cost ◽

Ease Of Use ◽

Gelatin Capsules ◽

Long Term Storage ◽

Pros And Cons ◽

Storage Methods ◽

Term Storage

Abstract Museums use gelatin capsules to store small objects and specimens, despite limited documentation of their long-term viability. The Royal Alberta Museum (RAM of Canada) uses gelatin capsules to store seeds, bones, and plant material because of their ease of use, transparency, soft-bodied walls, size availability, and low cost. Recently, RAM staff reported damaged capsules from the palaeontology collections. We evaluated 499 capsules used to store specimens accessioned in 1986 and 1988 and investigated capsule properties using Fourier transform infrared spectroscopy and Oddy testing. Only 4.21% of inspected capsules were dented, cracked, and/or fractured. Based on interviews and testing, we determined that damage to capsules likely resulted during handling (i.e., applied force when opening). We conclude that gelatin capsules offer a good, inexpensive method for long-term storage of small, dried specimens in environmentally controlled conditions. Alternatives to gelatin capsules exist, although their pros and cons require evaluation before use. All storage methods require continuous monitoring for signs of container or specimen deterioration.

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Performance of low‐cost stainless‐steel beverage kegs for long‐term storage integrity and easy dispensing of water isotope ( d 18 O, d 2 H) reference materials

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.9164 ◽

2021 ◽

Author(s):

Stefan Terzer‐Wassmuth ◽

Leonard I. Wassenaar

Keyword(s):

Stainless Steel ◽

Reference Materials ◽

Low Cost ◽

Long Term Storage ◽

Term Storage ◽

Water Isotope

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Development of a Synthetic Training Model for Canine Thoracocentesis

Alternatives to Laboratory Animals ◽

10.1177/0261192920924408 ◽

2020 ◽

Vol 48 (2) ◽

pp. 78-84

Author(s):

Nicolas Haller ◽

Jacob Reiss ◽

Frank Seipel ◽

Robert Weishar ◽

Zachary Alden ◽

...

Keyword(s):

Low Cost ◽

Training Model ◽

Immediate Feedback ◽

Veterinary Students ◽

Long Term Storage ◽

Procedural Complications ◽

Efficient Alternative ◽

Cadaveric Model ◽

Term Storage

Thoracocentesis, a procedure in which air or fluid is removed from the pleural space, is used to relieve respiratory distress, and as a diagnostic procedure in human and veterinary medicine. Veterinary students commonly learn and practice the procedure on canine cadavers which are in limited supply and are not amenable to long-term storage and use. Practicing thoracocentesis on a cadaveric model also provides limited feedback indicative of success and/or procedural complications. One commercial model for practicing canine thoracocentesis is available, but it costs over US$2000 and is excessively bulky. In order to improve the learning process for veterinary students, we have developed a reusable synthetic canine thorax model that accurately replicates the thoracocentesis procedure, provides immediate feedback to the students and reduces the need for canine cadavers. The low cost of our product provides an efficient alternative to cadavers for instruction in veterinary schools or hospitals.

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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