scholarly journals The effect of monocytes and T cells pretreated with aggregated IgG on generation of concanavalin A induced suppressor T cells

1983 ◽  
Vol 6 (1) ◽  
pp. 23-31
Author(s):  
Morihiro Ohara ◽  
Satoshi Igarashi ◽  
Chikara Kobashi ◽  
Masami Shirato ◽  
Masayuki Miyata ◽  
...  
Keyword(s):  
T Cells ◽  
Concanavalin A ◽  
Suppressor T Cells

scholarly journals Two distinct mechanisms regulate the in vivo generation of cytotoxic T cells.

1982 ◽  
Vol 156 (3) ◽  
pp. 918-923 ◽  
Author(s):  
M S Sy ◽  
S H Lee ◽  
M Tsurufuji ◽  
K L Rock ◽  
B Benacerraf ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Concanavalin A ◽  
Cytotoxic T Cells ◽  
Cytolytic T Lymphocytes ◽  
Spleen Cells ◽  
Suppressor T Cells ◽  
Con A

Treatment of responder cells with monoclonal anti-Ly-1,2 antibodies plus complement in vitro completely eliminated their ability to generate azobenzenearsonate (ABA)-specific cytolytic T lymphocytes (CTL). However, addition of the concanavalin A-stimulated supernatants of rat spleen cells (Con A-Sup) can fully reconstitute the response. Therefore, Lyt-1,2-bearing T cells are required for the generation of ABA-specific CTL, and such requirement can be replaced by factors present in the Con A- sup. Suppressor T cells (Ts), when adoptively transferred into naive recipients, will inhibit the in vivo priming of CTL. This inhibition can also be reversed by in vitro addition of Con A-Sup. furthermore, mice serving as donors of Ts also show profound unresponsiveness when primed and restimulated in vitro. In contrast to the Ts-mediated inhibition, in vitro addition of Con A-Sup was unable to abolish the unresponsiveness observed in these cultures. Thus, we identified two unresponsive states in a hapten-specific killing system that differ in their ability to be reconstituted by Con A-Sup.

scholarly journals Characterization of suppressor T-cells induced with concanavalin A in vivo by cell electrophoresis.

1988 ◽  
Vol 11 (3) ◽  
pp. 152-157 ◽  
Author(s):  
Motomu SHIMIZU ◽  
Minako NAKAMURA ◽  
Tateshi KATAOKA ◽  
Takao IWAGUCHI
Keyword(s):  
T Cells ◽  
Concanavalin A ◽  
Suppressor T Cells ◽  
Cell Electrophoresis

scholarly journals T cell growth factor abrogates concanavalin A-induced suppressor cell function

1981 ◽  
Vol 153 (5) ◽  
pp. 1360-1365 ◽  
Author(s):  
R Palacios ◽  
G Moller
Keyword(s):  
T Cells ◽  
T Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Concanavalin A ◽  
Cell Function ◽  
Suppressor T Cells ◽  
Con A ◽  
Suppressor Activity ◽  
T Cell Growth Factor

Concanavalin A (Con-A)-induced suppressor T cells were found to respond to T cell growth factor (TCGF) by proliferation. TCGF abrogated the suppressor activity exerted by these cells on phytohemagglutinin (PHA)- and alloantigen- induced lymphocyte proliferation and on pokeweed mitogen (PWM)-driven immunoglobulin secretion. The Con-A-activated suppressor T cells absorbed the TCGF activity, preincubation of these active suppressor cells with TCGF abolished their suppressor activity and addition of increasing numbers of Con-A-activated T cells reverted the abrogator,/ effect of TCGF. Altogether, these findings suggest that Con-A-induced suppressor T cells exert their function by decreasing the available levels of TCGF. Cyclosporin-A (CYA), which is known to inhibit the expression of receptors for TCGF on T cells, also inhibited the suppressor activity as determined in both indicator systems, namely PHA- or alloantigen-induced DNA synthesis and PWM-induced immunoglobulin synthesis. CYA made Con-A-treated T cells unresponsive to TCGF and unable to absorb the growth factor, supporting the notion that CYA inhibits the expression of TCGF receptors on T cells, a mechanism by which this drug seems to abrogate Con-A-induced suppressor T cell function.

scholarly journals Studies on Thermophile Products. IX. Isofatty Acid-Containing Phosphatidylglycerol That Enhances the Induction of Concanavalin A-Activated Suppressor T Cells.

1994 ◽  
Vol 17 (9) ◽  
pp. 1171-1175
Author(s):  
Yasuhiro KOHAMA ◽  
Taro SEMBA ◽  
Keishi TANAKA ◽  
Hideki TONE ◽  
Shinobu TANAKA ◽  
...  
Keyword(s):  
T Cells ◽  
Concanavalin A ◽  
Suppressor T Cells

In vitro effects of 4-hydroperoxycyclophosphamide on concanavalin A-induced human suppressor T cells

1984 ◽  
Vol 18 (2) ◽  
Author(s):  
Abraham Klajman ◽  
Ilana Drucker ◽  
Yosef Manor ◽  
Shlomo Ben-Efraim
Keyword(s):  
T Cells ◽  
Concanavalin A ◽  
Suppressor T Cells ◽  
In Vitro Effects

scholarly journals In vitro studies of the rabbit immune system. V. Suppressor T cells activated by concanavalin A block the proliferation, not the induction of antierythrocyte plaque-forming cells.

1976 ◽  
Vol 143 (4) ◽  
pp. 919-936 ◽  
Author(s):  
D Redelman ◽  
C B Scott ◽  
H W Sheppard ◽  
S Sell
Keyword(s):  
T Cells ◽  
B Cells ◽  
Concanavalin A ◽  
Peripheral Blood ◽  
Suppressor Cells ◽  
Spleen Cells ◽  
Suppressor T Cells ◽  
Con A ◽  
Activated T Cells

The late B-cell proliferative phase of the in vitro antibody response by rabbit spleen cells is highly susceptible to suppression by activated T cells. The in vitro antisheep erythrocyte plaque-forming cell (PFC) response by spleen cells from normal or primed rabbits can be suppressed by adding concanavalin A (Con A), Con A-prestimulated peripheral blood or spleen lymphocytes, or supernates from Con A-prestimulated peripheral blood lymphocytes. The suppression is not mediated by a direct interaction of Con A with responding cells as shown by the effectiveness of prestimulated cells. Primed spleen cultures remain sensitive to Con A suppression as late as 72 h after initiation, and the addition of Con A after 24-72 h rapidly stops the increase in the number of PFC. T cells are required for Con A addition to be effective but the suppression can be induced at a time when T-helper cells are no longer necessary. Further, the suppressive effect of Con A addition is abrogated by specific antisera to rabbit T cells. We propose that Con A activates suppressor T cells which then exert their effects on proliferating PFC or their immediate precursor B cells. The early inductive or recruitment phase of the response is probably not blocked by suppressor cells. Also, there is an apparent relationship between the number of proliferating B cells and the number of suppressor cells required. Finally, the difficulties in inducing a stimulatory effect by Con A and the prolonged period that Con A addition is suppressive suggests that the rabbit has relatively more and/or longer-lived suppressor cells than the mouse and may be a particularly useful species for studying suppressive phenomena and their mechanisms.

Lymphocyte electrophoresis as an indicator of modulation of concanavalin A-induced suppressor T-cells in vivo by anti-cancer drugs

1990 ◽  
Vol 12 (5) ◽  
pp. 545-551 ◽  
Author(s):  
Motomu Shimizu ◽  
Kiyomi Sekine ◽  
Tateshi Kataoka ◽  
Takao Iwaguchi
Keyword(s):  
T Cells ◽  
Concanavalin A ◽  
Cancer Drugs ◽  
Suppressor T Cells ◽  
Anti Cancer
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