scholarly journals T cell growth factor abrogates concanavalin A-induced suppressor cell function

1981 ◽  
Vol 153 (5) ◽  
pp. 1360-1365 ◽  
Author(s):  
R Palacios ◽  
G Moller
Keyword(s):  
T Cells ◽  
T Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Concanavalin A ◽  
Cell Function ◽  
Suppressor T Cells ◽  
Con A ◽  
Suppressor Activity ◽  
T Cell Growth Factor

Concanavalin A (Con-A)-induced suppressor T cells were found to respond to T cell growth factor (TCGF) by proliferation. TCGF abrogated the suppressor activity exerted by these cells on phytohemagglutinin (PHA)- and alloantigen- induced lymphocyte proliferation and on pokeweed mitogen (PWM)-driven immunoglobulin secretion. The Con-A-activated suppressor T cells absorbed the TCGF activity, preincubation of these active suppressor cells with TCGF abolished their suppressor activity and addition of increasing numbers of Con-A-activated T cells reverted the abrogator,/ effect of TCGF. Altogether, these findings suggest that Con-A-induced suppressor T cells exert their function by decreasing the available levels of TCGF. Cyclosporin-A (CYA), which is known to inhibit the expression of receptors for TCGF on T cells, also inhibited the suppressor activity as determined in both indicator systems, namely PHA- or alloantigen-induced DNA synthesis and PWM-induced immunoglobulin synthesis. CYA made Con-A-treated T cells unresponsive to TCGF and unable to absorb the growth factor, supporting the notion that CYA inhibits the expression of TCGF receptors on T cells, a mechanism by which this drug seems to abrogate Con-A-induced suppressor T cell function.

scholarly journals In vitro analysis of allogeneic lymphocyte interaction. V. Identification and characterization of two components of allogeneic effect factor, one of which displays H-2-restricted helper activity and the other, T cell-growth factor activity.

1981 ◽  
Vol 153 (1) ◽  
pp. 107-128 ◽  
Author(s):  
T L Delovitch ◽  
J Watson ◽  
R Battistella ◽  
J F Harris ◽  
J Shaw ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
B Cells ◽  
Growth Factor ◽  
Cell Growth ◽  
Spleen Cells ◽  
Con A ◽  
Effect Factor ◽  
T Cell Growth Factor

An allogeneic effect factor (AEF) derived from mixed lymphocyte reaction (MLR) cultures of alloactivated A.SW (H-2s) responder T cells and irradiated A/WySn (H-2a) stimulator spleen cells helps an in vitro primary anti-erythrocyte plaque-forming cell PFC response of BALB/c nude spleen cels and also A/WySn but not A.SW T cell-depleted spleen cells. AEF activity is adsorbed by anti-Ik and anti-I-Ak but not by anti-I-Jk, anti-I-ECk, and anti-Is. Gel filtration of ACA 54 resolves AEF into two main components that which appear in the 50,000- to 70,000-mol wt (component I) and 30,000- to 35,000-mol wt (component II) regions, respectively. Component I has a mol wt of 68,000, elutes from DEAE-Sephacel at 0.05-0.1 M NaCl, and has an isoelectric point (pI) of 5.8. It helps A/WySn but not A.SW B cells and, therefore, is H-2 restricted. Component II is not H-2 restricted, because it helps both A.SW and A/WySn B cells. It also stimulates (a) the growth of a long-term cytotoxic cell line in vitro, (b) Con A-induced thymocyte mitogenesis, and (c) the generation of cytotoxic T cells. The latter three properties of component II are not shared by component I. In addition, component II elutes from DEAE-Sephacel at 0.15-0.2 M NaCl and has a pI of 4.3 and 4.9. Ia determinants and Ig VH, CH, L-chain, and idiotypic determinants are not present on either component I or component II. The properties of component II are identical to that of a T cell growth factor produced by Con A-stimulated spleen cells. It is suggested that the H-2-restricted component I of AEF might be an MLR-activated responder T cell-derived Ia alloantigen receptor.

scholarly journals A new method for the detection of Lesch-Nyhan heterozygotes by peripheral blood T cell culture using T cell growth factor

Blood ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 912-916 ◽  
Author(s):  
N Kamatani ◽  
H Yamanaka ◽  
K Nishioka ◽  
T Nakamura ◽  
K Nakano ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Peripheral Blood ◽  
Normal Individuals ◽  
T Cell Growth ◽  
Hypoxanthine Guanine Phosphoribosyltransferase ◽  
T Lymphoblasts ◽  
T Cell Growth Factor

Abstract Thioguanine-resistant T lymphoblast populations were selectively amplified using T cell growth factor in the cultures of peripheral blood T cells from four Lesch-Nyhan heterozygotes. Although Lesch-Nyhan T lymphoblasts were all thioguanine-resistant, none of the cultures from 13 control subjects yielded the growth of such defective cell populations. These data provide direct evidence for the existence of a small percentage (5%–40%) of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) deficient T cells in the heterozygotes, but not in normal individuals. Conversely, culture of the T lymphoblasts with azaserine plus hypoxanthine permitted the growth of the other part of the cell population that was enzyme positive. The low percentages of HGPRT-negative cells among T cells in heterozygotes suggest that the presence of this enzyme is beneficial for differentiation of lymphocytes of T cell linkage. Considering the ease and the reliability, culture of the peripheral T cells with thioguanine and T cell growth factor is very likely of practical use for detecting Lesch-Nyhan syndrome carriers among predisposed females.

Regulation of the rate of cell cycle progression in quiescent cytolytic T cells by T cell growth factor: Analysis by flow microfluorometry

1984 ◽  
Vol 121 (1) ◽  
pp. 159-166 ◽  
Author(s):  
Rafick P. Sekaly ◽  
H. Robson MacDonald ◽  
Markus Nabholz ◽  
Kendall A. Smith ◽  
Jean-Charles Cerottini
Keyword(s):  
Cell Cycle ◽  
T Cells ◽  
Factor Analysis ◽  
T Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Flow Microfluorometry ◽  
T Cell Growth Factor

scholarly journals Factors that affect human hemopoiesis are produced by T-cell growth factor dependent and independent cultured T-cell leukemia-lymphoma cells

Blood ◽  
1982 ◽  
Vol 59 (6) ◽  
pp. 1330-1336 ◽  
Author(s):  
C Tarella ◽  
FW Ruscetti ◽  
BJ Poiesz ◽  
A Woods ◽  
RC Gallo
Keyword(s):  
T Cells ◽  
T Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
T Lymphocytes ◽  
Cell Lines ◽  
Humoral Factors ◽  
Human T Cells ◽  
T Cell Growth ◽  
T Cell Growth Factor

Abstract Some laboratory results and clinical situations suggest that human T cells may be important in the regulation of growth of hematopoietic cells. Since the discovery of T-cell growth factor (TCGF), systems are now available for the long-term specific in vitro propagation of mature normal or neoplastic human T cells, providing an opportunity to study the influence of T cells on hematopoiesis. Recently, 24 cell lines from patients with cutaneous T-cell lymphoma (CTCL) and T-cell acute lymphoblastic leukemia (T-ALL) were grown with TCGF and then assessed for release of humoral factors that affect hematopoiesis. Conditioned media (CM) from these cell lines were tested for erythroid burst- promoting activity (BPA) and granulocyte colony-stimulating activity (CSA). BPA was detected in CM from 3/6 cultures of T-ALL patients and 4/6 CTCL cultures. CSA was found in the CM from 6/8 cultures of T-ALL patients, 7/12 CTCL cultures, and 3/4 CTCL cell lines that become independent of exogenous TCGF for growth. The CSA from several of the neoplastic T-cell cultures stimulated high levels of eosinophil colonies, a possible source of the eosinophilia seen in these patients. The ability of continuously proliferating human T lymphocytes, which retain functional specificity and responsiveness to normal humoral regulation, to produce factors that directly or indirectly stimulate myeloid and erythroid colony formation lends further credence to the role of T lymphocytes in regulating hematopoiesis.

Tcgfiii/p40 is produced by naive murine cd4+ t cells but is not a general t cell growth factor*

1989 ◽  
Vol 19 (11) ◽  
pp. 2167-2170 ◽  
Author(s):  
Edgar Schmitt ◽  
Renate Van Brandwijk ◽  
Jacques Van Snick ◽  
Bernhard Siebold ◽  
Erwin Rüde
Keyword(s):  
T Cells ◽  
T Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Cd4 T Cells ◽  
T Cell Growth ◽  
T Cell Growth Factor
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