scholarly journals Confirmación taxonómica de Gelidium americanum (Gelidiaceae, Rhodophyta) en Tabasco, México, usando un enfoque morfológico y molecular

Author(s):  
Nataly Quiroz-González ◽  
Ma. Guadalupe Rivas Acuña ◽  
Ma. Edith Ponce Márquez

Antecedentes y Objetivos: Gelidium americanum se distribuye ampliamente en la costa Atlántica de México; sin embargo, hasta ahora no se han realizado estudios que confirmen su identidad taxonómica utilizando marcadores moleculares y caracteres morfológicos. El objetivo del presente trabajo fue confirmar la identidad taxonómica con un enfoque morfológico y molecular, de los especímenes identificados como G. americanum recolectados previamente en la laguna costera de Mecoacán, Tabasco, México.Métodos: Se recolectaron tres especímenes de G. americanum en la laguna Mecoacán, Tabasco, México. El material se analizó morfológicamente, mediante observaciones en un microscopio estereoscópico y microscopio óptico. Para el análisis molecular se extrajo ADN de los talos muestreados empleando el método CTAB; se amplificaron los marcadores rbcL y COI-5P con el kit Phire Plant Direct (PCR), las secuencias fueron editadas en Bioedit y alineadas en Clustal W. Enseguida se hizo un análisis de Máxima Verosimilitud en RaxML, otro de Inferencia Bayesiana en MrBayes y se calcularon las distancias genéticas en MEGA. Resultados clave: Las secuencias obtenidas en el presente estudio para los marcadores rbcL y COI-5P se anidaron en el clado de las muestras identificadas previamente con marcadores moleculares como Gelidium americanum. Además, las distancias genéticas fueron mínimas, los caracteres morfológicos coincidieron con lo descrito previamente para otras localidades del Atlántico, a pesar de contar con un limitado número de especímenes.Conclusiones: Utilizando marcadores moleculares y caracteres morfológicos, se confirma la presencia de Gelidium americanum en la laguna Mecoacán a pesar de presentar características ambientales diferentes a las que habitualmente se han descrito para esta especie.

2021 ◽  
Vol 11 (4) ◽  
pp. 1943
Author(s):  
Joo-Young Kim ◽  
Ju Yeon Jung ◽  
Da-Hye Kim ◽  
Seohyun Moon ◽  
Won-Hae Lee ◽  
...  

Analytical techniques such as DNA profiling are widely used in various fields, including forensic science, and novel technologies such as direct polymerase chain reaction (PCR) amplification are continuously being developed in order to acquire DNA profiles efficiently. However, non-specific amplification may occur depending on the quality of the crime scene evidence and amplification methods employed. In particular, the ski-slope effect observed in direct PCR amplification has led to inaccurate interpretations of the DNA profile results. In this study, we aimed to reduce the ski-slope effect by using dimethyl sulfoxide (DMSO) in direct PCR. We confirmed that DMSO (3.75%, v/v) increased the amplification yield of large-sized DNA sequences more than that of small-sized ones. Using 50 Korean buccal samples, we further demonstrated that DMSO reduced the ski-slope effect in direct PCR. These results suggest that the experimental method developed in this study is suitable for direct PCR and may help to successfully obtain DNA profiles from various types of evidence at crime scenes.


Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1489
Author(s):  
Tammy Stackhouse ◽  
Sumyya Waliullah ◽  
Alfredo D. Martinez-Espinoza ◽  
Bochra Bahri ◽  
Emran Ali

Dollar spot is one of the most destructive diseases in turfgrass. The causal agents belong to the genus Clarireedia, which are known for causing necrotic, sunken spots in turfgrass that coalesce into large damaged areas. In low tolerance settings like turfgrass, it is of vital importance to rapidly detect and identify the pathogens. There are a few methods available to identify the genus Clarireedia, but none of those are rapid enough and characterize down to the species level. This study produced a co-dominant cleaved amplified polymorphic sequences (CAPS) test that differentiates between C. jacksonii and C. monteithiana, the two species that cause dollar spot disease within the United States. The calmodulin gene (CaM) was targeted to generate Clarireedia spp. specific PCR primers. The CAPS assay was optimized and tested for specificity and sensitivity using DNA extracted from pure cultures of two Clarireedia spp. and other closely related fungal species. The results showed that the newly developed primer set could amplify both species and was highly sensitive as it detected DNA concentrations as low as 0.005 ng/µL. The assay was further validated using direct PCR to speed up the diagnosis process. This drastically reduces the time needed to identify the dollar spot pathogens. The resulting assay could be used throughout turfgrass settings for a rapid and precise identification method in the US.


2003 ◽  
Vol 47 (3) ◽  
pp. 39-43 ◽  
Author(s):  
B. Gilpin ◽  
T. James ◽  
F. Nourozi ◽  
D. Saunders ◽  
P. Scholes ◽  
...  

Identifying the source of faecal pollution is important to enable appropriate management of faecal pollution of water. We are developing and evaluating a combination of these microbial and chemical indicators better able to identify the source of faecal pollution. These assays make use of a combination of direct PCR, culturing, and colony hybridisation to identify source specific species of Bifidobacterium, Rhodococcus and Bacteroides. In conjunction with assays for (a) fluorescent whitening agents and (b) faecal sterols and stanols, these indicators were able to identify human derived faecal pollution in river water containing inputs from septic tanks, municipal oxidation ponds, farmed animals and feral animals. Differentiating amongst the animal sources was more difficult and will require development of molecular assays for organisms specific to each animal group.


Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Lishuang Shen ◽  
Lihuang Zhu

Direct PCR-based genetic mapping of telomeric repeat associated sequences (TASs) was achieved using a RAPD primer mediated asymmetric PCR method. Twenty-two TAS loci were mapped in a rice doubled haploid population derived from a cross between an indica variety (Zhaiyeqing8) and a japonica variety (Jingxi17). Of these, 11 loci were mapped to the most distal position of seven chromosome arms and lengthened the linkage groups by 7.4-22.6 cM, five were mapped to the approximate positions of the centromeric regions, and six were mapped to other interstitial chromosomal regions.Key words: rice, Oryza sativa L., genetic mapping, telomeric repeat, telomeric repeat associated sequences, RAPD primer mediated PCR.


1995 ◽  
Vol 89 (s33) ◽  
pp. 6P-7P
Author(s):  
V M Duke ◽  
P A De Zoysa ◽  
R Quinton ◽  
C S Smith ◽  
P-M G Bouloux

2013 ◽  
Vol 4 (1) ◽  
pp. e103-e104 ◽  
Author(s):  
Laila Hasap ◽  
Phuvadol Thanakiatkrai ◽  
Kamonnut Singkhamanan ◽  
Adrian Linacre ◽  
Thitika Kitpipit

2011 ◽  
Vol 3 (1) ◽  
pp. e103-e104 ◽  
Author(s):  
A. Barbaro ◽  
P. Cormaci ◽  
S. Votano
Keyword(s):  

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